U2 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101354

Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL23 [Human herpesvirus 6], codon optimized for human cell expression, NP_042893

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101354 represents NCBI reference of NP_042893 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTGTGGATCCCCTTTTCTGGGCATCTCCTCTTGGTCACTGGCATCCGCCGCGCTGTGCCCCCCAT
  CCTGTTCCTTCTCAGCCGGAAGGGATCTGAGGTGTGACGCGGAAGTCCCAGAGGTGAAATGGACTGCCTT
  TGTCCGGACTTTGGTTACAAGACCCCTCAGTGCCGATGATGTCTGGGATTTCGTTAGTACATTTGCACAC
  TGCCGGCTGGCATTGTCCTGGCCCGTTGGTGCGGAGCTTAGGTTCGCCACTTCAGATATGCTGGGGATCA
  CCCAGGCAGAGGTAGCCAAACTGTCTCGCCGCTACGGTTGTTGTCCGGGAATGGATCTGACAGTTATCGG
  CGTCACTATATTCGCGGAGGTATCTGCGCTGGTCCTTGTCGGTGAGTGTGGGGAAATCTACGCCTTTAAC
  GGGGTCTTCGATGATGCCCTGTATCGCCTGGCAGAGGATGCTTTCGGCCTGTGGAAACATGGATTGCGAC
  GGTTCGAACCTGTGTATGGGAGCAAATGCCTCACAGAGACTGGGGCAAGCTTTTTTGGAGGAATGAGCGG
  AGTAGACGACGTATTGGCATTCGCCGTGTCTTTCGACAAAGCTTTGGTGCCCCTCCCATGGCCCAGGGGA
  GCTTTTTTCGAATTTGCCGTCCCAGGCCGACCTGAGAAACGGTGGCGGCTCATTCCAGGCGGAGGCGTGG
  CCGTGGTTATCGGGAGGTTTTTCGGACGAGGAGTGACGTCTCCACTCCTGCGCCGGCAGAGGGTACTTAT
  GGACCAGGTGGGGCGAGTGTATGCAGCATCCTTGGATGGAGGCGCTGTAGTACGGCTGTCCGACTCCTTT
  CGGGCTTTTCTGGCTATGGGCGTAAGGAAGCTTTTCAAGAATCATAGATTCCCACCAGGGCACCTCTGGA
  CAATGCAGCTCCCTGTAACCTGTGTCCACGCCCCCGTGATAGATCTGCCAGCAGTGTACCAGTTGTCTCC
  GCACATGGTGGAACGGGGGATGCCTGTCGCCAGCTGTGAGGCCTCCACAGTGGTCCGGGGAGACTGTGAA
  GAAACCGAGACGGAGGAAAGCACTAGCGGCGACTCTGTCCTGGTGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101354 representing NP_042893
  Red=Cloning sites Green=Tags
  
  MFCGSPFLGISSWSLASAALCPPSCSFSAGRDLRCDAEVPEVKWTAFVRTLVTRPLSADDVWDFVSTFAH
  CRLALSWPVGAELRFATSDMLGITQAEVAKLSRRYGCCPGMDLTVIGVTIFAEVSALVLVGECGEIYAFN
  GVFDDALYRLAEDAFGLWKHGLRRFEPVYGSKCLTETGASFFGGMSGVDDVLAFAVSFDKALVPLPWPRG
  AFFEFAVPGRPEKRWRLIPGGGVAVVIGRFFGRGVTSPLLRRQRVLMDQVGRVYAASLDGGAVVRLSDSF
  RAFLAMGVRKLFKNHRFPPGHLWTMQLPVTCVHAPVIDLPAVYQLSPHMVERGMPVASCEASTVVRGDCE
  ETETEESTSGDSVLVC
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1098 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042893
• RefSeq ORF: 1098
• Locus ID: 1487881
• MW: 39.8 kDa