U82 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101428

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein L [Human herpesvirus 6], codon optimized for human cell expression, NP_042975

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U82
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101428 represents NCBI reference of NP_042975 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTTCTGTTGTTCGTAATGTCCCTTATTCTGCTCACATTCTCCAAGGCAATCCCCCTCTTCAACC
  ACAATAGTTTCTACTTCGAAAAGCTTGACGACTGCATAGCAGCCGTGATCAATTGCACTAAGAGTGAAGT
  CCCACTGCTTTTGGAGCCAATTTACCAGCCGCCCGCCTACAACGAGGACGTAATGTCTATTTTGTTGCAA
  CCACCCACCAAGAAAAAGCCATTCTCCAGGATCATGGTAACAGATGAATTTCTGTCCGACTTTCTGCTGC
  TCCAGGACAATCCTGAGCAGCTTCGCACCCTGTTCGCTCTCATACGGGACCCAGAAAGCCGAGATAACTG
  GCTCAACTTCTTCAACGGATTTCAGACCTGCTCCCCCAGCGTTGGAATCACTACATGCATCCGAGACAAC
  TGCCGCAAATATTCCCCTGAGAAGATCACCTATGTCAATAACTTCTTTGTTGATAATATTGCAGGGCTGG
  AATTCAACATCTCTGAGAACACTGACTCTTTCTACAGCAATATAGGGTTCCTCTTGTACCTGGAGAACCC
  GGCAAAAGGAGTGACTAAAATTATCCGATTCCCCTTCAACTCTCTGACCTTGTTTGACACCATCCTTAAT
  TGCCTGAAGTATTTCCACCTGAAGACCGGCGTTGAACTCGACCTCTTGAAGCACATGGAGACGTACAACA
  GCAAGCTGCCATTTCGGAGCAGTAGACCTACGATCCTGATCCGCAACACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101428 representing NP_042975
  Red=Cloning sites Green=Tags
  
  MELLLFVMSLILLTFSKAIPLFNHNSFYFEKLDDCIAAVINCTKSEVPLLLEPIYQPPAYNEDVMSILLQ
  PPTKKKPFSRIMVTDEFLSDFLLLQDNPEQLRTLFALIRDPESRDNWLNFFNGFQTCSPSVGITTCIRDN
  CRKYSPEKITYVNNFFVDNIAGLEFNISENTDSFYSNIGFLLYLENPAKGVTKIIRFPFNSLTLFDTILN
  CLKYFHLKTGVELDLLKHMETYNSKLPFRSSRPTILIRNT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 750 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042975
• RefSeq ORF: 750
• Locus ID: 1487962
• MW: 29.0 kDa