U84 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101430

Myc-DDK-tagged ORF clone of viral ORF for protein UL117 [Human herpesvirus 6], codon optimized for human cell expression, NP_042977

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U84
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101430 represents NCBI reference of NP_042977 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGATAGGGGAAATAAAAACACCAGTAAAACCCGGCAGACCGTGAAATCCAGGAGAACCCGAAAGA
  CCACAAAACAGTTCGTAGAACGGAAAAAGAACTTGAGCGCCAAGACCACCATTCGCAATAATAGACTGCA
  AAACAATTTGTCTCCACAGACAACCCCAACAGAAAAACCGGTAGATTACGCCACTGCCCGAGTGTTTCCG
  CAGCACACTCCCTACGACATTAAGTTTAACAAAGAAAAGTACAGGAACAACGAAATTTATGCCTACAGTA
  AACTCCGCAAGATCAGCAACGCCGTGTTCCGCCAAGAGAAAGAAATCAACCGACTCACCGTCTTCACGGA
  CAAGCCACTGGCCGACCTTTCCTTCAAGATGCCAGTGAACACTTCTACTACGGAGAAGAAGGAAATCGTC
  AGGAGTACCGAGTGCCAGCGGCAAGATAGAAACACCGTCAACGCTTTTAAGATTTTTCAGTCATATGAGA
  CTTCACCCCCATACTCCCCATGTAGCCCCAAGAATTTTATGTCCGAGATCTACAGGCTGTTCAGATGCTA
  TAACGCCTCTATTGTTCAGATTCAGGTGTACTCACGCAACAAGATTCTGATAAACGCTATGCAAGAAAAG
  CTGATGTCAATTGGGAACATGGTGATCAACGTTGGCGAACAGATCATCAGCGAGAGCGTGCACCATGAAC
  CCGTCGTGGCCATTGCAATCAACCAGTTCTTCAGGGGCACAGTGCCCCAGACAGACGCTCTGAGGAAGAA
  CGCCGTGACCCCTATCCCTGTGAATGCTAGCAAGAAAAAACTGGTTGGGATATGTAGTCTTATGATCAAT
  GCCCCAACCGAGAAGGACTTGCTGTGTGCGGCACACGTATGTATCAATTTCGCTATTTGCTATCCTAACG
  AAATTACTCTGAATATCGCGAAGCTGACTCAGCCAATGCTGCTGCAGGAGCACATAACCATATATAACTC
  CTTCAACAAGTATATCTACTGGGATGATTATGGGAAATTGCTTAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101430 representing NP_042977
  Red=Cloning sites Green=Tags
  
  MKDRGNKNTSKTRQTVKSRRTRKTTKQFVERKKNLSAKTTIRNNRLQNNLSPQTTPTEKPVDYATARVFP
  QHTPYDIKFNKEKYRNNEIYAYSKLRKISNAVFRQEKEINRLTVFTDKPLADLSFKMPVNTSTTEKKEIV
  RSTECQRQDRNTVNAFKIFQSYETSPPYSPCSPKNFMSEIYRLFRCYNASIVQIQVYSRNKILINAMQEK
  LMSIGNMVINVGEQIISESVHHEPVVAIAINQFFRGTVPQTDALRKNAVTPIPVNASKKKLVGICSLMIN
  APTEKDLLCAAHVCINFAICYPNEITLNIAKLTQPMLLQEHITIYNSFNKYIYWDDYGKLLN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1026 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042977
• RefSeq ORF: 1026
• Locus ID: 1487964
• MW: 39.6 kDa