U60 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101410

Myc-DDK-tagged ORF clone of viral ORF for DNA packaging terminase subunit 1 [Human herpesvirus 6], codon optimized for human cell expression, NP_042953

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101410 represents NCBI reference of NP_042953 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCGGACCTGCGACATAACACACATCAAAAACAATTATGAGGCAATCATTTGGAAGGGCGAGAGGG
  ACTGCAGCACTATATCCACAAAATATCCCAATTCCGCCATTTTCTACAAGAAGCGATTTATTATGCTGAC
  TCCAGAGCTCGGCTTTGCTCACTCATATAATCAGCAGGTGAAGCCCTTGTACACATTCTGCGAAAAACAG
  CGGCACCTGAAAAACCGCAAGCCACTGACTATACTCCCTAGCCTGTCTCATAAGCTGCAAGAGATGAAAT
  TCCTGCCAGCCAGTGACAAGAGCTTTGAGTCCCAGTATACTGAGTTCCTCGAGTCCTTCAAAATCCTTTA
  CCGGGAACCTCTCTTTCTGCAAATCGACGGGTTCATTAAAGACTTTAGGAAATGGATCAAAGGAGAATTC
  AACGACTTCGGAGATACTAGAAAGATCCAACTGGAACCTTTTCAGAAGAATATTCTGATACACGTGATCT
  TCTTTATTGCAGTGACTAAACTGCCGGCTCTGGCCAACCGCGTCATCAACTACCTGACTCACGTGTTTGA
  TATCGAATTTGTGAACGAAAGCACACTTAACACACTCAAGCAGAAAACGAACGTGTTCCTTGTGCCACGC
  AGGCACGGAAAGACCTGGTTTATTGTCCCCATTATCAGCTTCCTCCTGAAGAACATCGAGGGAATCAGTA
  TTGGATATGTAGCCCACCAAAAACATGTAAGCCACTTTGTCATGAAAGAAGTTGAGTTCAAGTGCCGGCG
  CATGTTTCCGGAAAAGACCATTACCTGCCTCGACAACGTAATTACCATCGACCATCAAAACATTAAGAGT
  ACCGCACTTTTTGCTTCCTGTTACAACACTCATTCTATCAGAGGCCAGTCCTTTAACCTCCTCATCGTGG
  ACGAGAGCCACTTTATCAAGAAGGATGCATTCTCCACAATTCTGGGTTTTCTGCCACAGGCTTCCACCAA
  AATTCTCTTTATCAGTAGCACAAACAGCGGGAACCATTCCACGAGCTTCCTCATGAAGCTCAATAATAGC
  CCCTTCGAAATGCTCTCCGTGGTCTCATACGTCTGTGAGGACCACGCTCACATGCTCAATGAGAGAGGTA
  ATGCTACCGCTTGTAGCTGCTACCGGCTGCACAAGCCGAAATTTATCTCCATTAATGCAGAGGTCAAAAA
  GACTGCAAATCTCTTTTTGGAGGGCGCCTTTATCCACGAAATCATGGGCGGGGCTACTTGTAATGTTATA
  AATGATGTGCTTATTACCGAGCAGGGCCAAACAGAGTTCGAGTTCTTCAGGTACAGTACCATTAACAAAA
  ATTTGATCCCATTTCTGGGGAAAGACTTGTACGTCTACCTCGACCCCGCTTACACCGGTAACAGAAGAGC
  AAGTGGCACTGGAATCGCAGCTATCGGGACTTACCTCGACCAATATATTGTTTATGGCATGGAGCATTAT
  TTTCTTGAGAGCTTGATGACCAGCTCAGATACAGCCATTGCAGAGTGTGCTGCCCACATGATCCTCTCAA
  TACTCGACCTGCACCCCTTCTTTACAGAAGTGAAGATAATCATAGAAGGAAACTCCAATCAGGCCTCTGC
  CGTGAAAATCGCCTGCATCATCAAGGAGAATATCACCGCCAACAAGAGTATTCAGGTGACCTTCTTTCAT
  ACCCCTGATCAAAACCAGATTGCCCAGCCTTTTTATCTGCTTGGTAAAGAAAAGAAGCTCGCAGTGGAGT
  TCTTCATCTCCAATTTCAACAGTGGTAATATCAAGGCAAGTCAAGAGCTGATTAGCTTTACTATTAAGAT
  TACTTATGACCCAGTGGAGTACGCACTGGAACAGATCAGGAATATCCACCAGATTTCTGTCAATAATTAT
  ATCACCTATTCCGCAAAAAAGCAAGCCTGCAGCGATGACCTGATCATCGCTATCATCATGGCCATTTATG
  TCTGTAGCGGTAATTCTTCAGCATCATTCCGGGAGATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101410 representing NP_042953
  Red=Cloning sites Green=Tags
  
  MLRTCDITHIKNNYEAIIWKGERDCSTISTKYPNSAIFYKKRFIMLTPELGFAHSYNQQVKPLYTFCEKQ
  RHLKNRKPLTILPSLSHKLQEMKFLPASDKSFESQYTEFLESFKILYREPLFLQIDGFIKDFRKWIKGEF
  NDFGDTRKIQLEPFQKNILIHVIFFIAVTKLPALANRVINYLTHVFDIEFVNESTLNTLKQKTNVFLVPR
  RHGKTWFIVPIISFLLKNIEGISIGYVAHQKHVSHFVMKEVEFKCRRMFPEKTITCLDNVITIDHQNIKS
  TALFASCYNTHSIRGQSFNLLIVDESHFIKKDAFSTILGFLPQASTKILFISSTNSGNHSTSFLMKLNNS
  PFEMLSVVSYVCEDHAHMLNERGNATACSCYRLHKPKFISINAEVKKTANLFLEGAFIHEIMGGATCNVI
  NDVLITEQGQTEFEFFRYSTINKNLIPFLGKDLYVYLDPAYTGNRRASGTGIAAIGTYLDQYIVYGMEHY
  FLESLMTSSDTAIAECAAHMILSILDLHPFFTEVKIIIEGNSNQASAVKIACIIKENITANKSIQVTFFH
  TPDQNQIAQPFYLLGKEKKLAVEFFISNFNSGNIKASQELISFTIKITYDPVEYALEQIRNIHQISVNNY
  ITYSAKKQACSDDLIIAIIMAIYVCSGNSSASFREI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1998 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042953
• RefSeq ORF: 1998
• Locus ID: 1487942
• MW: 76.2 kDa