U52 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101401

Myc-DDK-tagged ORF clone of viral ORF for protein UL79 [Human herpesvirus 6], codon optimized for human cell expression, NP_042945

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U52
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101401 represents NCBI reference of NP_042945 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCCAGATTGGACAATACATCAAGCTTAACGATGCAGTTCCTAACCTGATCCTTCATATTACCACAA
  AGCTGCTGCGGAATGAGAACCTTACCTCATTTAAACAAGAAGAACTTCTGCTCATCCAGCATGTTTGCAC
  ATCTATGCTCAGTCACGGCATTAAAATCTTGCTGTTGCGGGAGTCCCTTTACAACTCTGGGATTGGTGAC
  ATCGTGATCTTGAATCGCAAAATATCAAACAACTACTGGTTTAGAATTTTCTCCATTCTGAAGCAGCACT
  CTGATGCTGAGCTGCTCCGGCACATGTTCAATGAATCCCACTCTGCCTACATTAGCAAGAAGCTGCACTA
  CTCCGGTAACGTGTCCCACATGATTAATTTTCTCTTCATGGACGAGTTCGGAGTCAATCTGAAGATTCCA
  GAGGAGCTTATTTGCGAGGGAAACATTGTGTTCTCAGTTGGCGCTATCTACAATCATAGATTGTTGAAGA
  TCTGCAGATTTTTCAATAGGTTCTGGGGGGACCAGGAGCGCGAGCCTACCGTTCGACTGATCTGTAAGCA
  TCTGTGGTTTGCATACCTCATCATGTTTGGAAAATTCGAGATTAGCACACTGGCCTACTCTCAGCAGCGC
  GCCGAGCATAAAGCTGGCTTGTTCTCCTTTCTCCAAAACGACTTCAAGGTATTTTGTGGAATGAGTGAGA
  ATCCCCAACTCCTGGATAGCTCCGCTATATTTGATCTCACCGGTATATCAGCCGAGGATCTGTTTAGCTA
  TGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101401 representing NP_042945
  Red=Cloning sites Green=Tags
  
  MTQIGQYIKLNDAVPNLILHITTKLLRNENLTSFKQEELLLIQHVCTSMLSHGIKILLLRESLYNSGIGD
  IVILNRKISNNYWFRIFSILKQHSDAELLRHMFNESHSAYISKKLHYSGNVSHMINFLFMDEFGVNLKIP
  EELICEGNIVFSVGAIYNHRLLKICRFFNRFWGDQEREPTVRLICKHLWFAYLIMFGKFEISTLAYSQQR
  AEHKAGLFSFLQNDFKVFCGMSENPQLLDSSAIFDLTGISAEDLFSYE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 774 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042945
• RefSeq ORF: 774
• Locus ID: 1487932
• MW: 30.0 kDa