U11 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101359

Myc-DDK-tagged ORF clone of viral ORF for tegument protein pp150 [Human herpesvirus 6], codon optimized for human cell expression, NP_042902

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101359 represents NCBI reference of NP_042902 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCTGCAGCGGCATCCAATCCCCTTTGCATGGCTGGATAGAGACAAGGTTGAGAGGTTGACTGACT
  TTCTTTCAAATCTGGAGCGGCTCGACAATGTCGATCTGAGGGAACACCCCCACGTTACCAACTCCTGCGT
  GGTACGGGAAGGTGACGACGTAGATGATCTGAAAACCCTGTATAACTTGCTGGTGCTCTGGCTTATGTAT
  CACTATGTGCTTTCCAAGAGAAAGCCAGACTATAACGCAATTTGGCAGGATATCACCAAACTGCAGTCCG
  TGGTCAACGAGTACTTGAACTCAAAAGGTCTCAACAAGGGCATATTTGAGAATATGTTTACTAATAAGGA
  GAAATTTGAGAGCCAATTTTCTGATATCAATCGAGCACTTCTGCGGCTCGGGAATTTTATTAAATGGGGA
  AGTAACGTCGCTATCGACACACCTTATGTGAATCTGACTGCTGAGGATTCTTCCGAAATCGAGAATAACC
  TTCAGGACGCCGAAAAAAATATGTTGTGGTATACCGTATATAATATCAATGATCCTTGGGACGAAAATGG
  GTACCTCATAACATCCATAAACAAACTCATCTATTTGGGCAAACTCTTCCTGGCTCTTACTCAGAGTTGG
  AGTAAGCTGGAGAAGGTGGCCATGAGTCAGATAGTGATCACCCAGAATCACCTGTCAGGGCATCTTCGGA
  GACACGACAATTTCAACATCGTGTATTCACATCGGGTGCTTCAGACACCTCTGACCGGTCAGCGAGTAGA
  GTCCTTTCTTAAAATCATTACGTCCGACTACGATATTATCAAGAGCTCTCTGGAGAGTCACTCTGCGTCC
  AAGGCCTTTTCTATGTCAGAAATCGGACCCAACTCTCTGATGGACTTTGTCCCTCTTAGAGGCGATATCC
  ACAGCAACTTGACATTGCCATCCATGTCCATAGACACTAAGAAAAGCTCCCTGGACCCTGCCCGGTTGAA
  GAAATCCAATAGCCGATCCCTGGACTCTTTTCTTAGGATGCAGCGACAGCCTAAATTTCTCGAACTGGAT
  TCCGTCGACAACGCCGGTGAGAAAATTTTGCTGAAGGAAGCCACACTGGGCGGCGAGAATGTGAAGGCTA
  CTACCCCTGCCTCCAGTGTCTCCCTGATGTCCGGCGTAGAATCTCCCAGTTCCTTTACCAGCACTAACCT
  GGACCTGCCCCTCTCTTCATTTACATCCACTAACCTGGACCTGAGAGACAAAAGCCATGGCAACTACAAG
  ATTGGCCCTTCAGGGATACTGGACTTTAATGTGAAATTTCCTCCAAACGCCCAGCTGAATACCAATGGGG
  TGGATCTCCTGCAGGACAAAACTAGCATTGGGTCACCTAGTTCAGGAATCACAGACGTCGTCAATGGCTT
  CGCGAACCTTAATCTGCATCAGAACAAATCTAATGTCTCTCCACCATGGTCAAGAAATACAGCAGCAAAC
  GCTGACTTTCTTGATCCAGTCCATCGGTTCGTGCCTGAACAGACTGGCACCCCTTTCGTACTGAACAATA
  GCGACGTGGCCGGCAGTGAAGCGAAGCATACCACGTACTCTACTGAGACAGGAGTGTCTCCTCGGAATGT
  CTTCCTCATCAAAGACCTTAGAGGGAAGGACGGCTTTCGGAAACAGAAACAGAGCGACATCCCTAAATCC
  TTGACCAAGGAGAGGAACGACAAGGCCATCATGCACTCCCGAGAGGTTACTGGCGACAGCGGGGATGCGA
  CCGAAACCGTGGGCGCACGAAATAGCCCTGCGCTCCGCAAGATCAAGCAGGCCAATGACTTTTTTGCTGG
  ACTCAACAAGAAAAATGATAGGGACGTATTGCGGGGCGGCAAAGGTAACTCCAAGGACCTGCACAGCGGC
  GGAAATGCTAAAAAAAAGGAGATGTCCGGCAAATTTAACGATGACAAGGAAATGACACGGAACGGCCAGG
  AGCCTTCCAGAAGCCTGATGGGCGACGCCAGGAACGCCGGCGATGAGCAGTACATCCAGGCCGGCCTGGG
  ACAGAGAGTGAACAACCTGCTGTCTCAGTTTACAAACTTGATCAGCCTGGGAGAGAAGGGCATAGAAGAC
  ATCCTGCAGAACCAGCGGGGCACTGAGCTCAAACTGGCAACCGAGAATAAGTCCGGCAGGGAGAGCGAAG
  AGGCCAATGTCGAAAAAATCCTGGAAGTGTCCAACCCCCAGGATATGTTCAAAAACTTTAGGCTGCAGAA
  CGACCTGGATTCTGTACAGAGCCCCTTTCGGCTTCCGGATGCGGACCTGTCAAGAGAACTGGACTCCGCC
  AGCTTCAAAGACGCACTCGACCTTAAACTGCCTGGCAACGGGGAACGAGAGATCGACCTGGCCTTGGAGA
  AGGTGAAGGTTGGCGAGACCGAGACTTCAGATCTTAAGGTAGGGCAGGACGAGAGCTTCGTTCCTGCACA
  GCTGATGAAGGTGGAGACCCCCGAAGAGAAAGATGACATTATCGAACAGATGGTGCTGCGGATTAGGCAG
  GATGGCGAGACTGATGAGAACACGGTTTCCGGCCCTGGGGTGGCCGAGTCTCTTGACATTGAGGCGAAAG
  GAGAAAGTGCGATAGCAAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101359 representing NP_042902
  Red=Cloning sites Green=Tags
  
  MDLQRHPIPFAWLDRDKVERLTDFLSNLERLDNVDLREHPHVTNSCVVREGDDVDDLKTLYNLLVLWLMY
  HYVLSKRKPDYNAIWQDITKLQSVVNEYLNSKGLNKGIFENMFTNKEKFESQFSDINRALLRLGNFIKWG
  SNVAIDTPYVNLTAEDSSEIENNLQDAEKNMLWYTVYNINDPWDENGYLITSINKLIYLGKLFLALTQSW
  SKLEKVAMSQIVITQNHLSGHLRRHDNFNIVYSHRVLQTPLTGQRVESFLKIITSDYDIIKSSLESHSAS
  KAFSMSEIGPNSLMDFVPLRGDIHSNLTLPSMSIDTKKSSLDPARLKKSNSRSLDSFLRMQRQPKFLELD
  SVDNAGEKILLKEATLGGENVKATTPASSVSLMSGVESPSSFTSTNLDLPLSSFTSTNLDLRDKSHGNYK
  IGPSGILDFNVKFPPNAQLNTNGVDLLQDKTSIGSPSSGITDVVNGFANLNLHQNKSNVSPPWSRNTAAN
  ADFLDPVHRFVPEQTGTPFVLNNSDVAGSEAKHTTYSTETGVSPRNVFLIKDLRGKDGFRKQKQSDIPKS
  LTKERNDKAIMHSREVTGDSGDATETVGARNSPALRKIKQANDFFAGLNKKNDRDVLRGGKGNSKDLHSG
  GNAKKKEMSGKFNDDKEMTRNGQEPSRSLMGDARNAGDEQYIQAGLGQRVNNLLSQFTNLISLGEKGIED
  ILQNQRGTELKLATENKSGRESEEANVEKILEVSNPQDMFKNFRLQNDLDSVQSPFRLPDADLSRELDSA
  SFKDALDLKLPGNGEREIDLALEKVKVGETETSDLKVGQDESFVPAQLMKVETPEEKDDIIEQMVLRIRQ
  DGETDENTVSGPGVAESLDIEAKGESAIAS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 2610 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042902
• RefSeq ORF: 2610
• Locus ID: 1487891
• MW: 97.1 kDa