U53.5 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101403

Myc-DDK-tagged ORF clone of viral ORF for capsid scaffold protein [Human herpesvirus 6], codon optimized for human cell expression, YP_002608176

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U53.5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101403 represents NCBI reference of YP_002608176 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCAATCAAAACTTGCCCAATACCACTCCACTGCTGTCTAGTCCGCCATTCTCTGACTGTGTGTTTT
  TGCCAAAAGACGCCTTCTTCTCTCTGCTGAACGTGACTACCGGCCAGCAGCCTAAAATCGTTCCACCTGT
  TTCAGTGCACCCACCTGTGACCGAACAGTACCAGATGCTGCCATACAGCGAATCCGCCGCTAAAATAGCC
  GAACATGAAAGCAATAGGTACCACAGTCCTTGTCAAGCAATGTATCCTTACTGGCAGTATTCACCCGTGC
  CACAGTATCCTGCCGCACTGCACGGCTATCGGCAGAGCAAGACCCTGAAGAAGAGACACTTCCAGAGCGA
  CAGCGAAGATGAACTGTCCTTTCCTGGGGATCCCGAATATACCAAAAAGCGCCGGAGACACCGGGTTGAC
  AACGATGACGATAAGGAAATGGCCAGGGAAAAAAATGATCTGAGGGAGCTGGTGGATATGATCGGAATGC
  TGCGCCAAGAAATCAGCGCCCTGAAGCATGTGCGAGCCCAGAGCCCACAACGGCACATCGTGCCTATGGA
  GACCCTGCCAACTATCGAAGAGAAGGGTGCCGCAAGCCCCAAGCCAAGCATACTGAACGCCTCACTTGCC
  CCTGAAACCGTCAACCGGTCACTGGCAGGGCAGAATGAATCTACAGATCTCCTCAAGTTGAACAAGAAGC
  TGTTCGTTGACGCACTCAATAAGATGGACTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101403 representing YP_002608176
  Red=Cloning sites Green=Tags
  
  MSNQNLPNTTPLLSSPPFSDCVFLPKDAFFSLLNVTTGQQPKIVPPVSVHPPVTEQYQMLPYSESAAKIA
  EHESNRYHSPCQAMYPYWQYSPVPQYPAALHGYRQSKTLKKRHFQSDSEDELSFPGDPEYTKKRRRHRVD
  NDDDKEMAREKNDLRELVDMIGMLRQEISALKHVRAQSPQRHIVPMETLPTIEEKGAASPKPSILNASLA
  PETVNRSLAGQNESTDLLKLNKKLFVDALNKMDS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 732 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, YP_002608176
• RefSeq ORF: 732
• Locus ID: 1487935
• MW: 27.7 kDa