U79 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101426

Myc-DDK-tagged ORF clone of viral ORF for protein UL112 [Human herpesvirus 6], codon optimized for human cell expression, NP_042972

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U79
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101426 represents NCBI reference of NP_042972 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACGCAGAAGAACGGGCCTACGGAAGCTTTGATGACGTTATGGAGGTTTATCAGCAAATAGTGACTG
  AAAGTATCCAGTTGAAGCGGTTGCACTTCGGCTCTGGCTGTCTGATCGAGTTTCTTGGGGATTCTGGCAC
  TTGCGAGACCTTTTGTGGGGGATGGATCTCCATGATATGTTGGACAAGCGACACCAATTCCATGGGATCC
  CTCACAGTGGACATTGGGATTGATGACGGGAAGTACAAGACATACAACGCGCGCGGCTTTCTGCTGTGCA
  GCAAGAGTATCACCTCCATCAGCCAGAACACTGAAGGAAGAGACAGGATCCTGACGGTGTCACAGGAGAA
  TAATAAGCTCCAGATCACCTTTGTGACTCTTACCAAGGTGTTCAAGGAACACGATATTCGGAACCTCGGA
  GACCCCAAGTGTATCGAGAAATTCGAAAAAGAGTGTAGAGCCCTCGATAGAAAGAAGCACGACGATGAAC
  ACCGGAAACGCAGTGGCAAGCAGAAGGAAAAGCGGAAAGTCGAGGATACTGACAAGAAAAAGGACGACGA
  CCGGCGCAAACAGGAAGAGAGGAAACGGAACGACGAAGACAAACAGCCCGACAAAAAAGAAGAGTCTGAC
  GAACTCCCGAAAGAGAAGAGGCAAAAATACCACGATATGAAGCGGAATCTGGAGGAGCAGTCTCACGAAG
  ACGGAATCACTCTTACCTCCACTACACTGGTGAACGGTGCCGTTGAGGGCGCCCTCCCACCGTGTATTTC
  CATTGACAACCACGAGGATCAACAGCACGACGAGCTGGATAAAAGAGCCTACGCCCAAGGGACCAACCGC
  GAGGGCCTCAGCAACGAGGATAACTATGGGAACTTCCGGCTCAATAAATCCTTGGAACAGCTGAGAGCCA
  AGCTTGTAGCTTCATCCGGCGATATTGTAGAACGGTCCCTGCTGAAGTTGAAAGAGTGCCTGGATAACGT
  TAAAGACAATCTGATAAAGAACGAATGTGCAGATGTGACCGGACCTTCAAAATGCTTGAGCAAGACGAAG
  CATATAGAGCCCAAGAAACAGATTGTCTTCTCTGATTGCGTGAGGCCCGTTCCAGTGTGTGAAATAAAGC
  CATTCATTGATGTGCGCTTGTTCGAAACAGCTCGGAGCCCACGGCGCCTCAGGCAAAGAACACGGACAAT
  TGTGGGGTCCACGGACGGTGCCATTGAACAGCAGAGAGTGATCTCAGGGCAGAATAGGGGCCGCGCCCGC
  GGCCGGGGGAGAGGAAGGGCGCCGAGAAGAAGAAACTCTAACATCAACAACAGCCGGACTCAGACCACTA
  TAGTCATCGATGACAGTTCCGAAGCCGAGAACTTCGAGAATGAGGGATCATTCAATGAGGACCTGCTGGC
  CACCACAATTCTGGAGACGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101426 representing NP_042972
  Red=Cloning sites Green=Tags
  
  MYAEERAYGSFDDVMEVYQQIVTESIQLKRLHFGSGCLIEFLGDSGTCETFCGGWISMICWTSDTNSMGS
  LTVDIGIDDGKYKTYNARGFLLCSKSITSISQNTEGRDRILTVSQENNKLQITFVTLTKVFKEHDIRNLG
  DPKCIEKFEKECRALDRKKHDDEHRKRSGKQKEKRKVEDTDKKKDDDRRKQEERKRNDEDKQPDKKEESD
  ELPKEKRQKYHDMKRNLEEQSHEDGITLTSTTLVNGAVEGALPPCISIDNHEDQQHDELDKRAYAQGTNR
  EGLSNEDNYGNFRLNKSLEQLRAKLVASSGDIVERSLLKLKECLDNVKDNLIKNECADVTGPSKCLSKTK
  HIEPKKQIVFSDCVRPVPVCEIKPFIDVRLFETARSPRRLRQRTRTIVGSTDGAIEQQRVISGQNRGRAR
  GRGRGRAPRRRNSNINNSRTQTTIVIDDSSEAENFENEGSFNEDLLATTILETL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1422 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042972
• RefSeq ORF: 1422
• Locus ID: 1487960
• MW: 54.2 kDa