U14 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101362

Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL35 [Human herpesvirus 6], codon optimized for human cell expression, NP_042906

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101362 represents NCBI reference of NP_042906 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACTCACGCTATGGAAGAGCCAAAGACTTTCAATATTCCTACATTCGTCCTGGACGAAAACTGTA
  ACTTCATTCCCGAAGTTCTGTCACGGGCAAATGCCAAGTACATTAAGGATGTTTTGATACAGGACTCATA
  TAACACAGTCTGCCTCGCCAATTCTTTCATACCAATGACCATCCAGACCGCCGAGCAGATAATGGTCATT
  ATCACCAAATTTAAGTTTTCCCGCGCGCGAGACCTGTTCGAAAAGGTCCTTCGGCTTGGGGTACACATCA
  ATCGCTTCTATGCCGGGAAAAAGCAAGTCAAGCATATGATCACAATGATGAAGTCACTGTTTGACACTGA
  GGAGGCCATGAGACGATTGGATAGAGCTCTGATGGGACTGTTTGTGGACGCTCGCGGGTCCTCCTACATG
  CCTCTGATCGCGCTCAGCCTCCACGAAAATGGACTGCCCGATAGCAAGTTCACCAAGGCGGTAAAGCTCA
  TCAACACCACACTTAATAGCTTCCACAATAGACCAGACGCCGATATCGAACAGTATGCACAGAAGCTCCG
  GGCATACAATTACCTTTACAAGATACCTCATTATACTTTGAAAGAAGCCGTGGACGTCTATTCCGAGAAC
  CTGAAAGACCTTAGCATTGGAGTGAATAAGAAGCCCACCCTCCTGTTCACATCCTCCGACGATGGATATC
  TGTCACATATCTTTAATGACATGCTGTTCCTTACATCAACATGGAATATGATCTACAATTGCAAGGTGCA
  GATAAGGAGACTGACCACTTGGATCAAATATGAAATCAACTCAATCGTGGAGGACTGCGTGCTTGTTGGT
  TTCCAACTGCCCGACCTGAAGGATACTATCCTGGACCTGGCTGCCCTGACGTCTAACATGAACCTCGTCG
  ACCCAGACAAGGACCTGTTTTCCCACTATAAGCTTATCCTCGAGAAACTGTTTGAAATCAGCATCTTTGC
  AACTAAAGCTAATATTTGCATACTGCCTACATTCATAAAGAGTCACCTGATCGAATTTGAGGACGTGCTC
  AAAAGGTCCAATGATGACGAGGACCTCAACTACCTCCTTCTGAAGAGCAACGACTCCGATGACGAGTATG
  ACGAAGATAAACTGCCAATCCAAGTCGATCCCGGGAGGGTGGACAATGTACTGACCGATTCCAATTTTTT
  TAATGTTACTGCTGATAATGCTTTTTCTTCCATCGCTATCATGCCCATCAGCTACGACAAGATTATTGAC
  GTGGAAGAAAATGTCATCCAGGTCCTGGAAGTCGAAATGCAATCTCTTAGCGCCGTAGTTTATGGAGCCG
  TGGCTAACAAGTATGGACTCAGCTTGCCCCAGGTAATTAAGCGCCTGAACCAAAACGAGGGGCGGGCAAG
  TAGCAGAGCTAGTTCTTCCCACAGTACATCTACGATCCCTTATAGTCCTCCCCAGTCCGGACGGAGTACT
  CCCACAAGCATCCTGAGACAACGCGCACCTATTCGCTCCAACTCCCGCTCCTCTAGCGTCAGCTTCTCTC
  AAGACGATGACAACCGCTCTCACTATAGCGATGAGACCAACATCTCTGATTATTCCTACCCTATGGCTGA
  TCTGGACCTGGAAGATGAGGAACCGATGGAGGACCATCCTCATAGCCCACAATCCGCCAGTAGTAATAAC
  TCCATGAGTCGAAGATCCAGGGCTCTTCAGAACGAACAGAGGAGAAGAACCCCAACCATGGCCCCACCAT
  CACCCGCCAGAGGGCAGAATAATGCCCGGTTGCGACGCCGCACCAGACGGAGCACCGAAACAAATGACGA
  ACGACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101362 representing NP_042906
  Red=Cloning sites Green=Tags
  
  MVTHAMEEPKTFNIPTFVLDENCNFIPEVLSRANAKYIKDVLIQDSYNTVCLANSFIPMTIQTAEQIMVI
  ITKFKFSRARDLFEKVLRLGVHINRFYAGKKQVKHMITMMKSLFDTEEAMRRLDRALMGLFVDARGSSYM
  PLIALSLHENGLPDSKFTKAVKLINTTLNSFHNRPDADIEQYAQKLRAYNYLYKIPHYTLKEAVDVYSEN
  LKDLSIGVNKKPTLLFTSSDDGYLSHIFNDMLFLTSTWNMIYNCKVQIRRLTTWIKYEINSIVEDCVLVG
  FQLPDLKDTILDLAALTSNMNLVDPDKDLFSHYKLILEKLFEISIFATKANICILPTFIKSHLIEFEDVL
  KRSNDDEDLNYLLLKSNDSDDEYDEDKLPIQVDPGRVDNVLTDSNFFNVTADNAFSSIAIMPISYDKIID
  VEENVIQVLEVEMQSLSAVVYGAVANKYGLSLPQVIKRLNQNEGRASSRASSSHSTSTIPYSPPQSGRST
  PTSILRQRAPIRSNSRSSSVSFSQDDDNRSHYSDETNISDYSYPMADLDLEDEEPMEDHPHSPQSASSNN
  SMSRRSRALQNEQRRRTPTMAPPSPARGQNNARLRRRTRRSTETNDERL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1827 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042906
• RefSeq ORF: 1827
• Locus ID: 1487898
• MW: 69.6 kDa