U69 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101417

Myc-DDK-tagged ORF clone of viral ORF for tegument serine/threonine protein kinase [Human herpesvirus 6], codon optimized for human cell expression, NP_042962

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U69
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101417 represents NCBI reference of NP_042962 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAACGGGGTGGAGACCCCCCAGGGACAGAAGACCCAACCAATCAATCTGCCACCCGTCAGGAAGA
  AACTTAGAAAGCATGAAGGGCTGGGCAAGGGAGTGAAGCGCAAGCTGTTCGCCGAGGACTCCTCCCCCCT
  TAAAAAGCAGATCTCTGCATGTTCCGACATGGAAACACTCAGCAGTCCGGTAAAATCCGAATGTGAGTCT
  CGGTCTGCTTCTTTGGATGAGAGCTTTGGGAAATGTAAGCATGAGATCGCCTGCGACTGTAGCGCCATCG
  AAGAACTGCTTTGCCACGAAAGTCTCCTCGACAGTCCCATGAAGTTGTCAAATGCTCATACTATTTTCTC
  CAGCAATAAGTGGAAACTTGAACTCGAAAAAATCATAGCCTCCAAGCAAATCTTCCTGGACATGAGCGAA
  AATGCGGAGCTCGCCGCTTACGGAGAAACCCTCTGCAATCTGCGGATTTTCGAGAAGATTAGCTCTCCCT
  TTCTGTTTGACGTGCAGTCCGAGGAACGGAGCTACTCTGTCGTGTATGTACCCCACAACAAGGAGCTGTG
  CGGCCAGTTCTGCCAGCCCGAAAAAACAATGGCTAGGGTGCTGGGCGTGGGCGCATACGGGAAAGTGTTC
  GATCTTGACAAAGTGGCAATTAAGACTGCTAATGAAGACGAAAGCGTTATAAGCGCCTTCATCGCGGGCG
  TCATTCGAGCCAAATCCGGGGCGGATCTGCTCTCCCACGAGTGCGTTATCAATAACCTCCTGATATCCAA
  CAGCGTCTGTATGTCCCATAAGGTGTCCCTTTCCCGCACCTATGACATCGATCTCCACAAATTTGAGGAC
  TGGGACGTGCGCAACGTTATGAACTACTACTCCGTATTCTGCAAACTGGCCGACGCTGTGCGATTCCTGA
  ACCTGAAGTGCAGGATAAACCATTTTGATATCAGCCCAATGAATATCTTCCTTAACCATAAGAAGGAGAT
  TATCTTCGATGCTGTGTTGGCCGACTATAGCCTCTCTGAAATGCATCCAAACTACAATGGCACCTGTGCC
  ATCGCAAAGGAATACGATAAGAACCTGCAGCTCGTACCAATTTCAAGGAACAAATTCTGCGATATGTTCA
  ACCCAGGTTTTAGGCCGCTGGTCGCAAATGCGATGATCCTTGTGAATGTTTGTGGGGCTTTCGACGGTGA
  GAATAACCCCCTCCGACATTGTAACTTGGACTTGTGTGCATTCGCTCAGGTGGTGCTGTCATGCGTTCTC
  CGGATGACCGACAAACGCGGATGCCGGGAGGCACAACTTTACTACGAGAAGCGGCTGTTCGCCCTCGCGA
  ACGAAGCTTGCAGACTTAATCCCCTGAAGTACCCTTTCGCTTATAGAGATGCTTGCTGTAAAGTGCTTGC
  CGAACATGTGGTGCTGCTGGGTCTGCTTTTCTACCGGGACGTGGTGGAAATTTACGAGAAGCTGTACGAC
  TTCCTCGATGAGCGGGGGGAGTTTGGCTCCCGAGACCTGTTCGAGGCAACATTTCTTAACAACTCAAAAC
  TGACCAGACGACAACCTATCCGCGAAGGGCTCGCCAGCCTTCAAAGCAGTGAGTACGGCGAAAAACTGTT
  GCATGACTTGAGGGAACTCTTCCTCATTAACAGCACAGCAGACCTGGATAAGGACACTAGTTCTCTCTTC
  CATATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101417 representing NP_042962
  Red=Cloning sites Green=Tags
  
  MDNGVETPQGQKTQPINLPPVRKKLRKHEGLGKGVKRKLFAEDSSPLKKQISACSDMETLSSPVKSECES
  RSASLDESFGKCKHEIACDCSAIEELLCHESLLDSPMKLSNAHTIFSSNKWKLELEKIIASKQIFLDMSE
  NAELAAYGETLCNLRIFEKISSPFLFDVQSEERSYSVVYVPHNKELCGQFCQPEKTMARVLGVGAYGKVF
  DLDKVAIKTANEDESVISAFIAGVIRAKSGADLLSHECVINNLLISNSVCMSHKVSLSRTYDIDLHKFED
  WDVRNVMNYYSVFCKLADAVRFLNLKCRINHFDISPMNIFLNHKKEIIFDAVLADYSLSEMHPNYNGTCA
  IAKEYDKNLQLVPISRNKFCDMFNPGFRPLVANAMILVNVCGAFDGENNPLRHCNLDLCAFAQVVLSCVL
  RMTDKRGCREAQLYYEKRLFALANEACRLNPLKYPFAYRDACCKVLAEHVVLLGLLFYRDVVEIYEKLYD
  FLDERGEFGSRDLFEATFLNNSKLTRRQPIREGLASLQSSEYGEKLLHDLRELFLINSTADLDKDTSSLF
  HM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1686 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042962
• RefSeq ORF: 1686
• Locus ID: 1487950
• MW: 63.7 kDa