U30 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101378

Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL37 [Human herpesvirus 6], codon optimized for human cell expression, NP_042923

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101378 represents NCBI reference of NP_042923 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGCACGAGCCCTGGATCTGGAGTTCATCCTGAGTTCACTGAAAAATTCAACCTCACCTGAGTCCC
  TTCTGGCAGCCACTGCTAAAATCGAACTCTTGTCCTTGGCCGCCGATAGCGTAACTCACAATAGGGTTAT
  AGCCTTTATTAACCGACTGACACCTAAGAAATACCACTTTGATTTGATCAGACAATATGCTGTATTCTAT
  TTCCTGAATTCCACCACCCTTACCAGCGAGAATAAGCTGCTGAGTGCCGAACTCCTGTATGAGGAGCTGA
  GCCAAATTCCTTCCAGCAGCTCCTCAGGCACCGAGCTGGAGAACCTGAATAACGCTGAAGTGTTGTACGT
  TTTTGATAGAATACTCAATAGCATCAAGATGCTTAAAAACGAGCTCTCAAGCCCCATAGGCAAACTTAGA
  GCTCAGCCCGCTGCTAACGACCCCGGCACCGATAAGACTATTAAGTACTCCAAGATTCAGTCCATAATCC
  AGAAGATCAACAGTTTTACCCACGAGAATAGCCTCCTGGCTAACTGTAGAGCCGTGGTAGAGTTGATTGA
  TGACTTGTATAGAAAGCTTTATAGCTGGTTTCTGCATGTGCTGACATTTGAGGATATTCAGTTCCCAGGT
  GACACCTTCCTGGATAGGCTGCTCAAGATGGATTACTGTTTTACATATTACCCGTCCTCAAATAGACATC
  TGATCGACCTGTTTGAGAAGACGTTGGATAACCAGACCTTTACCAATTTGGATAAATTCTTTGATACCTC
  TGGAAACTCCCCAGAGTTGCTGTATCAGAAGACATTCAGTCTCAAGATATTTTCAAAAAACCTGACCGCC
  CAGGACAACGGATTGTACATCTATCCCCTGCTTAAAACTGACTTGTCAATCCTGGACTTTCTGGGCACAG
  AGAATATCCTTTTTCATAGGGGACTGATTTACCACATCTTGCACCAGAAGACTATCCCACAAGAGCGGGA
  GAACGACTTGAATAAGATTAACCAATTTTTTGCAACAGTCATCCAGCAGGTCATTGAAACCAAAAGTTCT
  TGTCTGCCCGCCAGCCTGTCCCAGCTCCTCGACACTATCTTTCACTTTAATCGCATCGGACTTAATATGG
  AGACCTGTAGGACTTACATCGAAATACTGTCCAACCACATGGCCACTCCTGATACCCAACCCATCATAAA
  CACATTTACTATCAATCTGACCCACATAGTGTTCACCGCACACGTCTTCTTTATTTGCATGGAGAATTTC
  AGCCCCACCTTCATCTTCTATAATAGAAAGAAATTGATCCTCGAACAGCAAAGAGCTATTCTCATCATCG
  AAAGGAACGATTACAGCACACTTTGGAAACAAATCTCTGATCATATCGAGTGCCTCTTTAACGTAAGCCT
  TTCAGAGAGCTTCTTCAAAGAGTACACCAAGGGTGGGAATGAGGATCAGAAACAGTTTCTCTATAAGAAT
  CTGTTCGAGAAATGGGGAAACGTCTTCTTCCCATTCACTTATAGCGTGTCCACTTCAAACAACTCTACTG
  CCCACCACATCACAACTCTTGAACTCCGCGATATCTGTAAAGAGGTGTATCAGTCTGATTCCCCTGATGC
  CTACGAGTCACTTCTTCCCTATAGTACCCACCCGAGCTTTAAGACACTGTTCGTCAAGATTTATGTGATC
  CCTATGGTTTCACACATCACCAACCTCACGTTTGACAAGTTGCAGTCCGACTGTCGGTTGCTGACTCTGA
  TACATGCCTGCAAGCTGCTGCTGCCTTCTCAGCACCTCTTGCTGCACTATATGGCTTGGCTTTACGCATT
  TTCAATAAATGTGGACCATATCGATCTCGGCACATTCACAGTGATCAAGTCCGTGATCTTCAAAATTGCA
  GATCACATCAATGTGATGACTCATACTATTTACTCTCCAGAGACAAATCTGCTCGTGAGCATCCTGCTGA
  ACGCTTATACGAACTACCTTCAGAAGTACGTGAACCCCTGGATCAAGCAGACCATCACAGCGAACTTTTC
  ACTCATTCAAACCTATATTACATTCACCAAACAGTGCGCTTCCATCCTGGCAACAAAGTGCAACATTAAT
  CTGGACAATCTGTTCATATACATCACAATTGGGACGGATAAGATTGTAACCACTTCATTCTGCAGTTTTA
  TCGCTACGTGCAGGAACCTGGTCCGACAGCACGAGGAGTTTGAGAAGAGTCTGCAGACGATTCAGATTTC
  AGAAATTACACTGACAGGCATGCTGAGAAACATCATCACAAGCGTCTCCTCCTCCAAGGAACTGTTGACC
  AACGAAGCATTGCAGAAATTCATCGACACGGTGCAGAGAATAAGCCAACACGTGAACGAGACGTATCATT
  CTATCTCAGTCAATCTGGAGAAATGTAAGACATCAAACGACATCCTCATTGAAAGTCTCAAAAAAATTAT
  CTACATTGTTGACGTCCTGAGCTCCAATGCCATTCTTAATACAAGCTTGGCTAGTCGGTGTCTGGAGGCC
  GCAAACCTGGCCGTATCTAATAACTCTTTTACAATTTTGGAAATTAAGAAAGACGCCGTGGCAGTCTTTA
  AACCGTTTATTACCCAGCTTTTCGAGAGCATGAAACCCACGACCAGTCTGCACAAAAAACTCATGTCTAC
  CCAGAAACTGACCACTGACCACATACCTTTTTTGGACACATTTGACGATAGATATAATCTGGTAAGGCAC
  GTCGAAAGGCAGCTTAATTGGTACGCTGCACACGCCGAGGCAGCCCAGCAGGATCTGATCACCCCTCTGA
  AGTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101378 representing NP_042923
  Red=Cloning sites Green=Tags
  
  MAARALDLEFILSSLKNSTSPESLLAATAKIELLSLAADSVTHNRVIAFINRLTPKKYHFDLIRQYAVFY
  FLNSTTLTSENKLLSAELLYEELSQIPSSSSSGTELENLNNAEVLYVFDRILNSIKMLKNELSSPIGKLR
  AQPAANDPGTDKTIKYSKIQSIIQKINSFTHENSLLANCRAVVELIDDLYRKLYSWFLHVLTFEDIQFPG
  DTFLDRLLKMDYCFTYYPSSNRHLIDLFEKTLDNQTFTNLDKFFDTSGNSPELLYQKTFSLKIFSKNLTA
  QDNGLYIYPLLKTDLSILDFLGTENILFHRGLIYHILHQKTIPQERENDLNKINQFFATVIQQVIETKSS
  CLPASLSQLLDTIFHFNRIGLNMETCRTYIEILSNHMATPDTQPIINTFTINLTHIVFTAHVFFICMENF
  SPTFIFYNRKKLILEQQRAILIIERNDYSTLWKQISDHIECLFNVSLSESFFKEYTKGGNEDQKQFLYKN
  LFEKWGNVFFPFTYSVSTSNNSTAHHITTLELRDICKEVYQSDSPDAYESLLPYSTHPSFKTLFVKIYVI
  PMVSHITNLTFDKLQSDCRLLTLIHACKLLLPSQHLLLHYMAWLYAFSINVDHIDLGTFTVIKSVIFKIA
  DHINVMTHTIYSPETNLLVSILLNAYTNYLQKYVNPWIKQTITANFSLIQTYITFTKQCASILATKCNIN
  LDNLFIYITIGTDKIVTTSFCSFIATCRNLVRQHEEFEKSLQTIQISEITLTGMLRNIITSVSSSKELLT
  NEALQKFIDTVQRISQHVNETYHSISVNLEKCKTSNDILIESLKKIIYIVDVLSSNAILNTSLASRCLEA
  ANLAVSNNSFTILEIKKDAVAVFKPFITQLFESMKPTTSLHKKLMSTQKLTTDHIPFLDTFDDRYNLVRH
  VERQLNWYAAHAEAAQQDLITPLKF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 2805 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042923
• RefSeq ORF: 2805
• Locus ID: 1487908
• MW: 107.5 kDa