U12 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101360

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein UL33 [Human herpesvirus 6], codon optimized for human cell expression, NP_042903

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101360 represents NCBI reference of NP_042903 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACCGTGATCGAGCTCAGTAAGCTGTTGAGGAATGAAGAGTTTAAAGGAAACGCCAGCTGCACGA
  GTACCCCTACCCTGAAAACCGCTCGGATCATGGAATCCGCCGTAACCGGTATCACCCTTACCACCTCCAT
  TCCTATGATCATAATTGTGGTCACAACTATGATCCTGTACCACCGCGTGGCCAAACATAACGCTACTTCC
  TTCTACGTCATTACCCTGTTTGCTAGTGACTTCGTGTTGATGTGGTGTGTGTTCTTCATGACAGTGAATA
  GAAAACAGCTCTTTTCCTTTAACAGGTTTTTCTGCCAGCTCGTATACTTTATATACCACGCTGTGTGTAG
  TTACTCAATCAGCATGCTTGCCATCATTGCCACTATCCGCTACAAGACACTTCATCGGCGCAAAAAAACA
  GAGAGTAAAACAAGCTCTACAGGTAGGAATATCGGGATCTTGCTTTTGGCTTCTTCCATGTGCGCCATTC
  CAACTGCACTGTTCGTGAAGACAAATGGCATGAAGAAAACGGGAAAGTGCGTCGTTTACATTTCCTCCAA
  AAAGGCCTACGAACTGTTTCTGGCAGTCAAAATCGTGTTCTCATTTATATGGGGGGTACTGCCCACCATG
  GTATTCTCATTCTTTTACGTGATCTTCTGTAAAGCTCTGCATGACGTGACAGAGAAGAAGTATAAGAAAA
  CCCTGTTCTTTATCCGCATACTTCTGCTCAGCTTCCTGCTCATCCAAATCCCCTATATTGCAATTCTCAT
  TTGCGAAATCGCCTTTCTGTACATGCCACAGAACACCTGTTTCTGGTTGGCCAGAGTCGAAATCCTGCAG
  CTGATTATTAGGCTTATGCCCCAGGTGCATTGTTTTAGCAACCCACTTGTTTACGCCTTCACTGGCGGCG
  AACTGAGAAATCGGTTCACCGCTTGCTTTCAAAGTTTCTTTCCAAAGACACTGTGCTCTACCCAAAAGAG
  GAAGGATTCAGACGCTAGTGAACACGACCAGAACTCTAAGTCCAAGGCCTCCGTGGAGAAGAATCAGCCT
  CTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101360 representing NP_042903
  Red=Cloning sites Green=Tags
  
  MDTVIELSKLLRNEEFKGNASCTSTPTLKTARIMESAVTGITLTTSIPMIIIVVTTMILYHRVAKHNATS
  FYVITLFASDFVLMWCVFFMTVNRKQLFSFNRFFCQLVYFIYHAVCSYSISMLAIIATIRYKTLHRRKKT
  ESKTSSTGRNIGILLLASSMCAIPTALFVKTNGMKKTGKCVVYISSKKAYELFLAVKIVFSFIWGVLPTM
  VFSFFYVIFCKALHDVTEKKYKKTLFFIRILLLSFLLIQIPYIAILICEIAFLYMPQNTCFWLARVEILQ
  LIIRLMPQVHCFSNPLVYAFTGGELRNRFTACFQSFFPKTLCSTQKRKDSDASEHDQNSKSKASVEKNQP
  L
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1053 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042903
• RefSeq ORF: 1053
• Locus ID: 1487893
• MW: 40.3 kDa