U29 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101377

Myc-DDK-tagged ORF clone of viral ORF for capsid triplex subunit 1 [Human herpesvirus 6], codon optimized for human cell expression, NP_042922

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U29
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101377 represents NCBI reference of NP_042922 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACTCCAAGTCTAGTGCTCGCGCCGCCATAGTAGATACCGTGGAGGCCGTGAAAAAGCGGAAATACA
  TCTCTATTGACGAGGGCACACTGAACAATGTCGTGGAGAAGGAGCGAAAGTTCCTGAAACAATTTTTGAG
  CGGCCGCCAGAACCTGCGAATCGCTGCAAGAGTATTTACCCCTTGTGAGCTGCTGGCACCAGAACTGGAA
  AACCTGGGAATGCTGATGTATAGATTTGAGACCGATGTTGACAATCCCAAAATCCTGTTTGTGGGCCTCT
  TCTTCCTCTGTAGTAATGCCTTTAACGTGAGTACTTGCGTTCGGACAGCCCTCACGGCTATGTATACCAA
  CTCCATGGTCGATAATGTCCTTTCTATGATTAATACCTGTCGCTATCTTGAAGATAAGGTGTCACTGTTC
  GGCGTCACAAGCCTCGTGTCATGTGGGAGCTCATGTCTCTTGTCCTGTGTCATGCAGGGGAATGTGTACG
  ATGTTAATAAGGAGAACATCTACGGTCTGACTGTTCTGAAGGAGATAATACTGGAACCCGACTGGGAGCC
  AAGGCAGCATTCCACGCAGTATGTGTACGTCGTCCATGTATACAAGGAGGTGCTGGCGAAACTGCAGTAC
  GGTATTTACGTGGTCCTTACATCATTCCAGAATGAGGACTTGATCGTCGATATCCTGAGACAGTACTTTG
  AAAAAGAGCGGTTTCTGTTTTTGAACTACCTGATCAATTCCAACACCACCCTGAGTTACTTCGGGTCAGT
  TCAGCGCATTGGACGATGTGCCACCGAAGACATCAAGAGCGGTTTCTTGCAGTATCGGGGGATAACCTTG
  TCAGTCATCAAGCTGGAAAATATCTTCGTCGACCTTTCAGAGAAGAAAGTTTTTGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101377 representing NP_042922
  Red=Cloning sites Green=Tags
  
  MNSKSSARAAIVDTVEAVKKRKYISIDEGTLNNVVEKERKFLKQFLSGRQNLRIAARVFTPCELLAPELE
  NLGMLMYRFETDVDNPKILFVGLFFLCSNAFNVSTCVRTALTAMYTNSMVDNVLSMINTCRYLEDKVSLF
  GVTSLVSCGSSCLLSCVMQGNVYDVNKENIYGLTVLKEIILEPDWEPRQHSTQYVYVVHVYKEVLAKLQY
  GIYVVLTSFQNEDLIVDILRQYFEKERFLFLNYLINSNTTLSYFGSVQRIGRCATEDIKSGFLQYRGITL
  SVIKLENIFVDLSEKKVFV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 897 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042922
• RefSeq ORF: 897
• Locus ID: 1487906
• MW: 34.2 kDa