U100 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101437

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein Q [Human herpesvirus 6], codon optimized for human cell expression, NP_042993

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U100
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101437 represents NCBI reference of NP_042993 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACAGCCCGCTTGAGTGCTATGAAACCACCTCGCTCTTGTGCCCTGATCTTCCTGTGCGCATTCA
  GTATGGCTACAGCTCCAACTAATGCCACCGCTCACAGACGGGCGGGCACAGTGAAATCCACGCCACCTCC
  TGAAGACAAACATAGCTACACGGCAAAGTATTATGATAAGGATATCTATTTTAACATATACGAGGGACGG
  AACAGCACTCCTCGCCGCAGGACACTTTCAGAAATTATTAGTAAGTTTAGTACTAGCGAAATGCTTAGTC
  TGAAAAGAGTGAAGGCTTTCGTTCCCGTGGATGAGAACCCCACAACAACGCTCGAAGACATCGCGGATAT
  TCTGAACTACGCCGTTTGCGATGACAACAGTTGTGGCTGCACCATTGAGACGCAGGCCAGAATAATGTTT
  GGCGACATCATTATCTGTGTGCCACTCTCAGCCGACAATAAGGGCGTACGAAACTTCAAGGACCGGATAA
  TGCCGAAGGGCCTGAGTCAGATCCTCTCCTCTTCCCTTGGCCTGCACCTGAGTCTGCTGTACGGCGCCTT
  CGGGAGCAACTATAATAGCCTGGCCTATATGCGGAGACTGAAGCCTCTGACAGCTATGACAGCTATCCGC
  TTTTGTCCCATGACCACGAAACTGGAATTGAGACAAAATTACAAAGTCAAGGAGACGTTGTGTGAGCTGA
  TTGTGTCTATTGAGATCCTGAAAATCCGGAACAATGGAGGACAGACCATGAAGACTTTGACATCCTTTGC
  CATTGTCAGGAAAGATAATGACGGGCAGGACTGGGAAACCTGTACTCGGTTTGCCCCCGTAAACATCGAG
  GACATTCTTAGATATAAGAGGGTCGCCAATGACACCTGCTGTCGGCATCGCGATGTGCAGCATGGACGAA
  GGACACTGGAGAGTAGCAACAGTTGGACACAGACCCAGTATTTCGAGCCGTGGCAGGATATTGTTGACGT
  GTACGTGCCAATCAACGATACACACTGCCCCAACGATTCTTACGTGGTATTTGAGACTCTGCAGGGATTC
  GAGTGGTGCTCTCGACTTAATAAGAACGAGACGAAGAATTATCTCAGCTCTGTCCTGGGTTTCCGAAACG
  CACTGTTCGAGACGGAGGAACTCATGGAGACTATTGCCATGCGGCTTGCATCTCAGATCCTCAGCATGGT
  CGGTCAGCAGGGCACCACCATTAGGGACATCGACCCTGCCATAGTGTCAGCGTTGTGGCACAGTCTCCCG
  GAAAACCTTACCACGACTAACATAAAGTACGACATCGCAAGCCCCACACATATGAGGCCGGCTTTGTGCA
  CAATATTTGTTCAAACCGGAACATCAAAACAGAGATTTCGCAATGCTGGGCTGCTCATGGTGAACAATAT
  TTTCACAGTGCAGGGCCGCTACACGACCCAGAATATGTTTGAACGCAAGGAGTACGTCTATAAACATCTG
  GGTCAGGCACTTTGCCAGGACGGGGAGATCTTGTTTCAGAATGAAGGACAGAAGTTCTGTAGACCCCTGA
  CCGACAACAGGACTATCGTTTATACCATGCAGGATCAGGTACAGAAGCCGCTGAGCGTGACTTGGATGGA
  CTTTAACCTTGTGATTTCAGACTACGGAAGGGATGTAATCAACAACCTGACTAAGTCCGCAATGTTGGCA
  CGCAAAAACGGACCTCGGTATCTCCAGATGGAAAATGGTCCAAGATATCTTCAGATGGAAACCTTCATCA
  GCGATCTTTTTCGGCACGAGTGTTATCAGGACAATTATTATGTGCTGGACAAGAAGCTGCAGATGTTCTA
  CCCCACCACACATAGCAATGAACTGCTGTTCTACCCTTCAGAGGCGACGTTGCCCTCCCCATGGCAGGAG
  CCACCGTTCAGTTCCCCCTGGCCGGAGCCAACCTTCCCAAGCCGGTGGTATTGGCTTCTGCTGAACTACA
  CAAACTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101437 representing NP_042993
  Red=Cloning sites Green=Tags
  
  MATARLSAMKPPRSCALIFLCAFSMATAPTNATAHRRAGTVKSTPPPEDKHSYTAKYYDKDIYFNIYEGR
  NSTPRRRTLSEIISKFSTSEMLSLKRVKAFVPVDENPTTTLEDIADILNYAVCDDNSCGCTIETQARIMF
  GDIIICVPLSADNKGVRNFKDRIMPKGLSQILSSSLGLHLSLLYGAFGSNYNSLAYMRRLKPLTAMTAIR
  FCPMTTKLELRQNYKVKETLCELIVSIEILKIRNNGGQTMKTLTSFAIVRKDNDGQDWETCTRFAPVNIE
  DILRYKRVANDTCCRHRDVQHGRRTLESSNSWTQTQYFEPWQDIVDVYVPINDTHCPNDSYVVFETLQGF
  EWCSRLNKNETKNYLSSVLGFRNALFETEELMETIAMRLASQILSMVGQQGTTIRDIDPAIVSALWHSLP
  ENLTTTNIKYDIASPTHMRPALCTIFVQTGTSKQRFRNAGLLMVNNIFTVQGRYTTQNMFERKEYVYKHL
  GQALCQDGEILFQNEGQKFCRPLTDNRTIVYTMQDQVQKPLSVTWMDFNLVISDYGRDVINNLTKSAMLA
  RKNGPRYLQMENGPRYLQMETFISDLFRHECYQDNYYVLDKKLQMFYPTTHSNELLFYPSEATLPSPWQE
  PPFSSPWPEPTFPSRWYWLLLNYTNY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1968 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042993
• RefSeq ORF: 1968
• Locus ID: 1487972
• MW: 75.6 kDa