U54 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101404

Myc-DDK-tagged ORF clone of viral ORF for tegument protein pp65 [Human herpesvirus 6], codon optimized for human cell expression, NP_042947

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U54
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101404 represents NCBI reference of NP_042947 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCCGCTACACTCCAATGGTCCTCCTATGTGCTTCAGTTGAGAGTGACCACCACCGCGATACTTA
  AACCCGGCGAACTCCGCTTGTTTAAATGCGGATTGGGCATCACGCCACCTTCCAGTGCCGTTGTTTGCAT
  TTGTCGAGATGAATCTTCCTTCACTTCCAGTCCGTTCACGTATATTGACCCGAAGGACTATGGCAATATC
  CCATTCGCAGTGCAGAATATCAGCGACCTCGATCTCGACTTGTCTCGGCTTCCCATTGTTCTCAACATCT
  TCGCCTTGCCCCATGCCAATGTTAATATCTCCAATTTGCCAGTGCAGCGAATTGAGGCCCATGACAGACA
  TATCATCCCACATGGGCAGTGTGACGCCAAATTCGTGATCTATGGACCTCTGACTCGCATTAAAATACAG
  GTGGCAGATATCCGATGGATCGAGCAGACACCCGAAGAGCCAACGAGGTACAGTTTTAAAGCAGAGATCT
  GGGTGAACCTCCAAAACACGCCTCTTGACCAGATCTTTAAGACCGGGAACATAGAGTTCATCAGTCATAA
  GAATGTATACATATCAAGAATCCTCCTTTGCGGCAACCTCCTTATCCTGAAGGCCAATTACGAAAACGAC
  TATCTCCTGGACGATAACTTTTACCCAGAACAGCTGGCCATCCAAATTAGCCTGACCCCACAGACCGCCG
  ACATAACGTTGAGTCAGAATCAGGAAACATTGCTTAAATGCAACACCCATAGCATCACCGTGTGTGCTAC
  TAAGAACATCATACCGAATACTGTTACACCTGTTCACTGCTCCTTCAACACAATCTTCAATTCCAACAGC
  AACTTCACAGGCCTGTTTATACCAAAGCTCCTGCTGGGCATCAGCACGACGACAGGAATTTGGGATGAGA
  CACGGCCGCTCTATATAACCATGAAAGCTTTCAAGAAAAATAGGCGCATCAATTATTCACAGCCGATCGG
  TGTTGTTTACTTTTTTCCTAAACAGATCTTGCCACCAGGAAACAACGTTGAGTTCAATTGGACAGAAGCC
  TCCAAAATTTACGTTAATACAGAGTCACCCAACGGGCCTGTCCGCAACACAGTGACATTCACAAACCAGG
  CCGTGCTTCGCACCCCCAGCCTGAGCACGGTTGCCAACCTGACTCCTGACATAAACATGGGAATATTCCT
  CTCAAGCCTCAGGGTGGCCTTTGACACTGCGCACATGGTTCCTCTGCACTTTAGCCTGAAGCCAGGAGAG
  TCTACTAGGATGGAATTCATGCCCCCTGGGACCCCTCAGAATCTGACCATTCTCGAGGGCGATGTTGGTA
  TCCACTTCATACCCTGTCACAACCACTCACATCGGTCCTCTCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101404 representing NP_042947
  Red=Cloning sites Green=Tags
  
  MQPATLQWSSYVLQLRVTTTAILKPGELRLFKCGLGITPPSSAVVCICRDESSFTSSPFTYIDPKDYGNI
  PFAVQNISDLDLDLSRLPIVLNIFALPHANVNISNLPVQRIEAHDRHIIPHGQCDAKFVIYGPLTRIKIQ
  VADIRWIEQTPEEPTRYSFKAEIWVNLQNTPLDQIFKTGNIEFISHKNVYISRILLCGNLLILKANYEND
  YLLDDNFYPEQLAIQISLTPQTADITLSQNQETLLKCNTHSITVCATKNIIPNTVTPVHCSFNTIFNSNS
  NFTGLFIPKLLLGISTTTGIWDETRPLYITMKAFKKNRRINYSQPIGVVYFFPKQILPPGNNVEFNWTEA
  SKIYVNTESPNGPVRNTVTFTNQAVLRTPSLSTVANLTPDINMGIFLSSLRVAFDTAHMVPLHFSLKPGE
  STRMEFMPPGTPQNLTILEGDVGIHFIPCHNHSHRSSP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042947
• RefSeq ORF: 1374
• Locus ID: 1487936
• MW: 51.5 kDa