U57 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101407

Myc-DDK-tagged ORF clone of viral ORF for major capsid protein [Human herpesvirus 6], codon optimized for human cell expression, NP_042950

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U57
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101407 represents NCBI reference of NP_042950 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAACTGGCAGGCGACAGAGATCCTTCCCAAAATCGAGGCCCCTCTCAATATCTTCAACGACATAA
  AAACATATACAGCCGAGCAGCTCTTCGATAATCTTCGCATATATTTCGGCGATGACCCTAGCCGGTATAA
  CATATCTTTCGAGGCCCTCCTGGGGATCTATTGTAATAAGATCGAGTGGATTAATTTCTTTACGACTCCC
  ATTGCCGTGGCAGCGAACGTGATCCGATTCAATGACGTCTCCAGGATGACCTTGGGAAAGGTGCTGTTCT
  TTATCCAACTGCCCAGGGTGGCCACAGGCAATGATGTGACAGCTAGCAAGGAAACCACCATCATGGTGGC
  CAAGCACTCAGAAAAACACCCAATTAATATATCCTTTGACCTTAGTGCCGCATGTCTGGAGCACCTGGAG
  AACACTTTCAAGAACACTGTCATCGATCAGATCCTGAACATTAATGCGCTGCACACTGTCTTGCGCTCCC
  TCAAAAACTCCGCAGACTCCCTGGAGCGGGGCCTGATCCATGCATTCATGCAAACACTCCTGAGGAAAAG
  TCCCCCCCAGTTCATCGTTCTTACAATGAATGAAAACAAGGTTCATAACAAACAGGCCCTGAGTAGAGTT
  CAACGCTCTAATATGTTTCAAAGCTTGAAAAATAGGCTGCTGACAAGTCTGTTCTTTCTTAACCGCAATA
  ATAACATCTCATATATTTACCGGATTTTGAACGATATGATGGAGTCAGTCACCGAGTCAATCCTTAACGA
  TACCAACAACTATACCTCAAAGGAAAATGTGCCTCTCGATGGGGTGCTGCTCGGACCCATCGGATCTATT
  CAGAAGCTGACAAGTATTCTTTCCCAATACATTTCAACACAGGTGGTGTCCGCGCCAATCAGTTATGGCC
  ACTTCATAATGGGCAAGGAGAATGCAGTGACGGCAATTGCTTATCGGGCTATAATGGCTGACTTTACACA
  GTTCACTGTGAATGCAGGAACAGAACAGCAAGACACCAATAACAAGTCCGAAATCTTCGATAAAAGCCGG
  GCCTACGCGGACCTGAAGCTGAACACCCTGAAGTTGGGCGATAAACTGGTCGCTTTTGACCATTTGCATA
  AGGTGTATAAAAACACGGATGTAAACGATCCACTCGAACAGTCCTTGCAGCTGACTTTCTTTTTCCCTCT
  CGGCATATACATACCTAGTGAGACAGGGTTCTCCACTATGGAGACGCGCGTCAAGCTGAACGATACAATG
  GAGAATAACCTTCCGACGAGCGTGTTCTTTCATAACAAGGATCAGGTGGTACAAAGGATCGACTTCGCAG
  ATATACTGCCTAGCGTGTGTCACCCAATTGTGCACGATTCAACCATTGTAGAGAGGCTGATGAAGAGTGA
  GCCCCTCCCAACAGGCCACAGGTTCAGCCAGCTGTGCCAGCTGAAGATCACTAGGGAAAATCCTGCCCGC
  ATTCTCCAAACTCTGTACAATCTCTATGAGTCACGGCAGGAGGTTCCTAAAAACACAAACGTCCTGAAGA
  ACGAGCTCAATATAGAGGACTTCTATAAGCCTGACAACCCCACCCTGCCCACTGAGCGACATCCATTCTT
  CGATCTTACTTACATCCAAAAGAATCGGGCTACCGAGGTGCTGTGTACTCCACGAATCATGATTGGAAAC
  ATCCCACTCCCTCTGGCCCCAGTGAGCTTCCATGAAGCCCGCACCAACCAAATCTTGGAGCACGCAAAGA
  CAAATTGCCAGAAGTATGACTTCACACTCAAGATCGTGACCGAGAGCCTGACTAGCGGCTCCTATCCCGA
  GCTGGCATACGTGATCGAGACGCTGGTCCATGGCAACAAGCACGCCTTCATGATACTGAAGCAGGTGATC
  AGCCAATGCATAAGCTACTGGTTTAATATGAAACACATCCTCCTGTTCTGCAACTCCTTCGAAATGATCA
  TGCTGATCTCTAATCACATGGGGGATGAGCTTATCCCGGGCGCGGCTTTCGCCCACTATAGAAACCTCGT
  GAGCCTCATTAGACTGGTGAAGCGGACTATCAGCATAAGCAATTTGAATGAGCAGCTCTGCGGCGAACCC
  TTGGTGAACTTTGCGAACGCTCTCTTTGATGGCCGATTGTTTTGCCCTTTCGTCCATACTATGCCGCGCA
  ATGACACTAATGCTAAAATCACTGCAGATGATACCCCTCTGACCCAGAATACCGTGCGGGTTCGCAACTA
  CGAGATTTCTGACGTTCAAAGAATGAACCTTATCGATTCCTCTGTGGTCTTTACTGATAACGACCGACCT
  TCCAACGAGACCACGATTCTGTCTAAAATTTTCTACTTCTGCGTGCTTCCAGCTCTCTCCAACAACAAGG
  CATGTGGGGCTGGTGTCAACGTCAAGGAGCTGGTACTCGACCTTTTCTACACCGAGCCTTTCATTAGTCC
  CGACGACTACTTTCAGGAAAATCCAATCACAAGTGACGTGCTGATGTCACTGATTAGAGAGGGGATGGGT
  CCAGGTTATACCGTTGCAAATACCAGCTGTATTGCCAAACAGCTGTTCAAGTCTCTGATCTACATTAACG
  AGAATACAAAGATTCTGGAGGTAGAAGTGTCCCTGGATCCAGCCCAGCGCCATGGAAACAGCGTGCACTT
  TCAAAGTTTGCAGCACATACTGTACAATGGCCTCTGCCTTATCAGCCCTATAACCACCTTGCGAAGGTAT
  TACCAGCCCATACCCTTCCATAGATTTTTTTCTGACCCTGGGATCTGCGGGACCATGAATGCGGACATTC
  AGGTTTTTCTCAATACTTTTCCACATTGTCAGCGCAATGACGGAGGTTTCCCGCTGCCACCTCCCCTGGC
  ACTCGAGTTTTATAATTGGCAGAGAACGCCGTTTAGCGTGTACAGCGCTTTTTGCCCGAACTCACTTCTG
  TCTATCATGACACTGGCAGCTATGCATAGTAAGCTGAGTCCGGTGGCAATCGCCATCCAATCCAAAAATA
  AGATCCATCCTGGATTTGCCGCTACCCTGGTAAGGACCGATAATTTCGACGTAGAGTGTCTGCTCTACAG
  TTCACGCGCCGCCACTTCTATAATCCTGGATGACCCTACCGTGACCGCCGAAGCTAAGGACATCGCAACC
  ACATATAATTTTACCCAACACCTGTCATTTGTTGATATGGGGCTCGGGTTCAGCTCCACCACTGCAACCG
  CCAATCTGAAAAGGATCAAATCAGACATGGGGTCAAAAATCCAGAACCTTTTTAGCGCGTTCCCGATTCA
  CGCTTTCACCAATGCAGATATAAACACCTGGATTCGGCACCACGTCGGGATCGAGAAGCCTAACCCATCA
  GAAAGCGAAGCCCTGAACATTATCACCTTCGGTGGAATCAATAAGAACCCTCCTTCTATCCTCTTGCACG
  GACAACAGGCTATTTGTGAGGTTATTCTGACACCAGTGACTACCAACATCAATTTCTTCAAGTCCCCACA
  CAACCCCCGAGGGAGGGAGTCATGCATGATGGGAACTGACCCACACAACGAAGAGGCTGCCAGAAAAGCT
  CTCTATGATCATACTCAGACCGACTCTGACACCTTCGCGGCAACAACTAATCCCTGGGCAAGTCTCCCCG
  GGTCACTGGGCGACATACTGTACAACACTGCTCACAGGGAGCAGCTCTGCTATAATCCAAAAACCTACTC
  ACCAAACGCTCAATTCTTTACCGAGTCAGACATCCTTAAGACCAACAAAATGATGTACAAGGTGATTTCC
  GAATATTGCATGAAATCCAATTCATGTCTGAACAGCGATTCTGAGATTCAATATTCCTGCTCCGAAGGCA
  CTGATTCCTTTGTAAGCAGGCCATGTCAATTTCTGCAGAATGCATTGCCCCTTCACTGTTCCAGTAACCA
  AGCCCTCTTGGAGTCCCGCTCTAAAACAGGTAACACACAAATTAGCGAAACCCACTACTGTAACTATGCC
  ATCGGTGAGACGATCCCTTTTCAGCTCATCATCGAGTCCTCCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101407 representing NP_042950
  Red=Cloning sites Green=Tags
  
  MENWQATEILPKIEAPLNIFNDIKTYTAEQLFDNLRIYFGDDPSRYNISFEALLGIYCNKIEWINFFTTP
  IAVAANVIRFNDVSRMTLGKVLFFIQLPRVATGNDVTASKETTIMVAKHSEKHPINISFDLSAACLEHLE
  NTFKNTVIDQILNINALHTVLRSLKNSADSLERGLIHAFMQTLLRKSPPQFIVLTMNENKVHNKQALSRV
  QRSNMFQSLKNRLLTSLFFLNRNNNISYIYRILNDMMESVTESILNDTNNYTSKENVPLDGVLLGPIGSI
  QKLTSILSQYISTQVVSAPISYGHFIMGKENAVTAIAYRAIMADFTQFTVNAGTEQQDTNNKSEIFDKSR
  AYADLKLNTLKLGDKLVAFDHLHKVYKNTDVNDPLEQSLQLTFFFPLGIYIPSETGFSTMETRVKLNDTM
  ENNLPTSVFFHNKDQVVQRIDFADILPSVCHPIVHDSTIVERLMKSEPLPTGHRFSQLCQLKITRENPAR
  ILQTLYNLYESRQEVPKNTNVLKNELNIEDFYKPDNPTLPTERHPFFDLTYIQKNRATEVLCTPRIMIGN
  IPLPLAPVSFHEARTNQILEHAKTNCQKYDFTLKIVTESLTSGSYPELAYVIETLVHGNKHAFMILKQVI
  SQCISYWFNMKHILLFCNSFEMIMLISNHMGDELIPGAAFAHYRNLVSLIRLVKRTISISNLNEQLCGEP
  LVNFANALFDGRLFCPFVHTMPRNDTNAKITADDTPLTQNTVRVRNYEISDVQRMNLIDSSVVFTDNDRP
  SNETTILSKIFYFCVLPALSNNKACGAGVNVKELVLDLFYTEPFISPDDYFQENPITSDVLMSLIREGMG
  PGYTVANTSCIAKQLFKSLIYINENTKILEVEVSLDPAQRHGNSVHFQSLQHILYNGLCLISPITTLRRY
  YQPIPFHRFFSDPGICGTMNADIQVFLNTFPHCQRNDGGFPLPPPLALEFYNWQRTPFSVYSAFCPNSLL
  SIMTLAAMHSKLSPVAIAIQSKNKIHPGFAATLVRTDNFDVECLLYSSRAATSIILDDPTVTAEAKDIAT
  TYNFTQHLSFVDMGLGFSSTTATANLKRIKSDMGSKIQNLFSAFPIHAFTNADINTWIRHHVGIEKPNPS
  ESEALNIITFGGINKNPPSILLHGQQAICEVILTPVTTNINFFKSPHNPRGRESCMMGTDPHNEEAARKA
  LYDHTQTDSDTFAATTNPWASLPGSLGDILYNTAHREQLCYNPKTYSPNAQFFTESDILKTNKMMYKVIS
  EYCMKSNSCLNSDSEIQYSCSEGTDSFVSRPCQFLQNALPLHCSSNQALLESRSKTGNTQISETHYCNYA
  IGETIPFQLIIESSI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 4035 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042950
• RefSeq ORF: 4035
• Locus ID: 1487939
• MW: 152.0 kDa