U65 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101414

Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL16 [Human herpesvirus 6], codon optimized for human cell expression, NP_042958

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U65
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101414 represents NCBI reference of NP_042958 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATATCAACTTTCAGCATAGGGGATCTCGGCTATCTGCGGAACTTCCTGCAGAATGAATGCAATT
  GGTTTCGGATATGCAAGAAAACTTTTTACCGGGAATATCGGTCTGTTGCCACATCATCACCCACTTTCTC
  CCTCAATAACAAACCCAAAAAGTTCTGCATGCACTGTGAGATCGTGATTTTTAAACGGTCCGAAGAGTTC
  ATGTTCTCCCTGGCCGTGAACGGAATTCACTTCGGGCAGTTTTTGACTGGGAAAATGAAATTCAATAAGA
  AAGCCGTGCCCGAGGGACTCTACTACTACATTCTCGAGCTGGGTTCCATTACGCCTATTGATCTGGGATT
  CATTCCCCGGTACAACTCAGACTGCGTTACCAACATGCGGTGTGTGACACCCGAAGTGATCTACGAGAAT
  TGCAGCATCGTTTGTCCGGAAGAGGCGAACAGGTTGACTGTCAAGGGGAGTGGGGATAACAAACTGACAC
  CTCTGGGTGGATGTGGTGCCTGGTGTCTCAAGAACGGTGGTGACTTGTATATCTATACATTCGCTCTCGC
  GTATGATCTCTTTCTTACCTGCTATGACAAATCTACTTTTCCCTCTTTGGCCAAGATAATCTTTGACATG
  ATCGCCTGTGAGAGCGAAGACTGCGTCTTCTGCAAAGACCACAACAAGCATGTTAGCCAGGCAGGCCAGA
  TTGTAGGTTGTGTGAGCAACCAGGAAACCTGCTTCTGCTATACTAGCTGCAAGAAAAAAATGGCTAATAT
  CAATAACCCAGAGCTGATTAGCTTGCTGTGCGATCAGGAAATCAACAAAATCGACATTATGTATCCAAAG
  ATTAAGGCAAGCCTGTCCCTTGATATTAACAGCTACGCTCATGGTTATTTCGGAGATGATCCATACGCTT
  TGAAATGCGTCAATTGGATCCCAGTGAGAATATCCGCCGCCTTGTCCCGATTGATTGTCCTCAGTTGTCC
  AGTGTGTAAGCGCGTAGTCATGGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101414 representing NP_042958
  Red=Cloning sites Green=Tags
  
  MAISTFSIGDLGYLRNFLQNECNWFRICKKTFYREYRSVATSSPTFSLNNKPKKFCMHCEIVIFKRSEEF
  MFSLAVNGIHFGQFLTGKMKFNKKAVPEGLYYYILELGSITPIDLGFIPRYNSDCVTNMRCVTPEVIYEN
  CSIVCPEEANRLTVKGSGDNKLTPLGGCGAWCLKNGGDLYIYTFALAYDLFLTCYDKSTFPSLAKIIFDM
  IACESEDCVFCKDHNKHVSQAGQIVGCVSNQETCFCYTSCKKKMANINNPELISLLCDQEINKIDIMYPK
  IKASLSLDINSYAHGYFGDDPYALKCVNWIPVRISAALSRLIVLSCPVCKRVVMD
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1005 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042958
• RefSeq ORF: 1005
• Locus ID: 1487947
• MW: 37.9 kDa