U34 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101382

Myc-DDK-tagged ORF clone of viral ORF for nuclear egress membrane protein [Human herpesvirus 6], codon optimized for human cell expression, NP_042927

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101382 represents NCBI reference of NP_042927 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAATGTACTGAAAGAAAAAATGTATGACGAACTGCTCTCAGCCACATGCCGAATACTGAAACTGG
  GAAGCCATGATTATAGAATCACAGAACGGAACCTTCTCTCAAAGAATCCAAAGTTTCCTCTGTGCGATAT
  CATTTTGAAGCTTGATTACGCCTATAACCTGGAATATCTGCTGAGTCTGTGGGAGCACGTGACCAAGCAA
  GAGCCTCGGTTTGTCTTTAAAAACACTGGCGGCGCAGTAAGCATGAGCTGCTATCTCCATGCGCCCGTAA
  AGGTCGAGGGCCACCATGCAGTGCGGGAATGCAACATCCTGAGAGTTAACGAGTGTCTGACCGTCCGCAT
  GAGCGATATTGTGGCTATGAAGCCCTCTACATTTGCAGTGTTCACCAAGTGTATTATCAGACGGAATAGA
  GATGATACATACGTGGTAGAGTTTGTCGCCTTTGGACCCGAGAATGAGTCAGAATATATATCACTCCTGA
  AAGCCATCTTTTTGAAGAAGTGCTCCATGGGAAAGCAGCACCTTGAATCCAATAGGTTCTGCCAGGGACT
  GCGAAGAAGGAGCAGCCACGTACTCGAGAAGGGTCGGTTTGAATCATCCGGCAAAGTGGTCAATAAGGCT
  TCTGCCGTAGTTACCTCACAGGAGAGTATTAAGCAGTTCTATGAGAAAGAGAAATCACTGCTTAGCGGAG
  TGAAGTTTTGGAGGCTTTCCGAACGCCACTGTAGATTTGCGCTGGTTGGAATATGCTTTCTCTTGGCCTT
  GTACTTCTGTTACGTCCTCCTGAAGAAGACTCCTACTCCTGCATCCGGCTCCGTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101382 representing NP_042927
  Red=Cloning sites Green=Tags
  
  MANVLKEKMYDELLSATCRILKLGSHDYRITERNLLSKNPKFPLCDIILKLDYAYNLEYLLSLWEHVTKQ
  EPRFVFKNTGGAVSMSCYLHAPVKVEGHHAVRECNILRVNECLTVRMSDIVAMKPSTFAVFTKCIIRRNR
  DDTYVVEFVAFGPENESEYISLLKAIFLKKCSMGKQHLESNRFCQGLRRRSSHVLEKGRFESSGKVVNKA
  SAVVTSQESIKQFYEKEKSLLSGVKFWRLSERHCRFALVGICFLLALYFCYVLLKKTPTPASGSVV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 828 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042927
• RefSeq ORF: 828
• Locus ID: 1487911
• MW: 31.7 kDa