U4 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101356

Myc-DDK-tagged ORF clone of viral ORF for protein UL27 [Human herpesvirus 6], codon optimized for human cell expression, NP_042895

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101356 represents NCBI reference of NP_042895 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACTGCTTGACCATGATATTTATAAGGGACCCGTGAGGGAGAGAGTGACCTACACGATCCCCAACC
  ATCCCTATCTGAGCCTTACCGTTCACCATAGCCGGGAGCTGGACGTGGACCTTAAGGACATCACGGAAGA
  AATGATTATCGACTCTGGAACACTGACAGCCGAGGATCTCTTTATGACTAGGGGACTTAGATTCTGCGAC
  GACTCCGTCCTGTGGGCTGCTCTGGCTGAGAAAGTAAGTGTGGAGTTCCAGAGGCGCGGAAAGACTACGA
  TGGATCTGTCCGCATTTATGTCTTTGTTGGAGAGACTGACTTTCGAGGATGAGACTTCTGTCTTCTATAG
  GCTCCTTACCAAATGCACAGCCTTGGCACATTTTTCCATCCTGGAGTATATCGTCGACGGCGAGAAGCGC
  GATACAATTAGTGCCCACTTCAACCGGCTCCTGGAACTCCTTGATTCTCTGTTTCTCCAGTTCCTCATGC
  TCAGAAATCGCAGTGAGAGCAACACCCTGCTTACCCTGTTCGACATCTTGCCAAACCCTAAGGAGATCTA
  TGGGGATGCCGCCTCACCCACTAGTGTGCTGCTCAAGCACTTCCTCTCTGACCACATATTCGTGGCCTAC
  GCATCAAGTTACCGGTTCGTCTCTTCAGTTCTCTGCAGTTGTGACCAGTGTCGCGCGAGCCTGTTTCGGA
  TTTATCTTGGAAAGAAAGATTCTAGGGCAGTTCACATCAGCGATATTAGCGAATTCGATCCTGCGGATTT
  GATTCTGGGCCAGCTGCATCTTGACGCAAGGGAAGCCGTAGCACTGCGCGCCGAAATAGACCGCGACTTG
  GGATCTTCCCTGATCCTGAACAGTATGGAACGGAGGCACCTCCCCATCCTGCATGATAAACAGCTCGGCC
  GCGGACACTTTGAACGCAATATCCTCAAGGTGTACTGCAATATCGTTTTGTGTTTGTTTCTGCTGCGGCG
  GGTAAAGCAGAGAATCGTATCCGACCTCACTGCGATTGCAAGATTCTTTGTCAAGGCTATCAGCGAAATG
  GAACGGTGTATTGATACCGTATCCGGACTTAGAGGGGTAAGAATCAAACTCCTCGTGGTCAGCAGCCAGT
  TTGAGATCAAGGACCCCATGCGCGTGCCTCGATTGTGTTTCTTTTTCCTGGAGATCGTTGCCATTTTGGT
  GAGCGGGTACGATAAGGAAAGGCACCGAGTGAGAGCGCTGCTGGAACACCTGTGTCTGAAGAAAATCTGC
  CTGGGAGACCTGTGCGCATCCATGAGACTGGCGAGAGAAATCCGCCATGATGTGCTCGAGGGCTTCCTGA
  ATACCCTGGAGCTGAGTTATATGAGTAACCCTGTGAGGTTCTTCCGGTTTCACGACTGTAATCTGTACGG
  AACCCACATGTGGAGCGGGGTGTACCCAAATGTGGTTCCATATGCTGTTAGAGGAGGTGGCTCCTTTGAC
  ATCAGACTGCAAGAACATAGACGAAGACAAAAGCGCATTGTCGTTAGGCGGAGGCTGATCCGCCGCGTCC
  AACAACGCGGTCTGGATATTCGCGAAGCTCGGCGCGTCCCTAAGCCCGCCTGCGTCACTTTCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101356 representing NP_042895
  Red=Cloning sites Green=Tags
  
  MELLDHDIYKGPVRERVTYTIPNHPYLSLTVHHSRELDVDLKDITEEMIIDSGTLTAEDLFMTRGLRFCD
  DSVLWAALAEKVSVEFQRRGKTTMDLSAFMSLLERLTFEDETSVFYRLLTKCTALAHFSILEYIVDGEKR
  DTISAHFNRLLELLDSLFLQFLMLRNRSESNTLLTLFDILPNPKEIYGDAASPTSVLLKHFLSDHIFVAY
  ASSYRFVSSVLCSCDQCRASLFRIYLGKKDSRAVHISDISEFDPADLILGQLHLDAREAVALRAEIDRDL
  GSSLILNSMERRHLPILHDKQLGRGHFERNILKVYCNIVLCLFLLRRVKQRIVSDLTAIARFFVKAISEM
  ERCIDTVSGLRGVRIKLLVVSSQFEIKDPMRVPRLCFFFLEIVAILVSGYDKERHRVRALLEHLCLKKIC
  LGDLCASMRLAREIRHDVLEGFLNTLELSYMSNPVRFFRFHDCNLYGTHMWSGVYPNVVPYAVRGGGSFD
  IRLQEHRRRQKRIVVRRRLIRRVQQRGLDIREARRVPKPACVTFI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1605 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042895
• RefSeq ORF: 1605
• Locus ID: 1487885
• MW: 62.0 kDa