DR6 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101439

Myc-DDK-tagged ORF clone of viral ORF for US22 family [Human herpesvirus 6], codon optimized for human cell expression, NP_043000

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DR6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101439 represents NCBI reference of NP_043000 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCACTCGACATACCCAGAGAACAGGGGGACGCATTTCCGTTCGGCGGAACGAAGCCCGGCTGGCGC
  ACGCCCGAGCCAGAGCCAGGTTCGATTGGTTGCTGTTGGCACGGGGCCGCCCCTCCAAGCTGTATGGCTA
  CGCTTCTCGCCATCGCGGTGAGCTGATCCACCTTCCCTGGCCTCCCTGCTGGTGTCTTGAGCTCCACCAC
  GATCCTTATAGAGATGCTAGATCTGCAACAGTGTGGGGCCACAGATGGGGCTGGCCTGCCACCCACGTGC
  GGCCCCGCTGTGTTCAGGATTGCGCCCTCGACAGTTGCCTCTACGTGTGTTGTGGGTACGGGGAAAAGTT
  GCAGCCTGTCGGTTTCGTGAAGTCATATGTGACGAACAGTCAGCTGGATACGCTTAGAGTGCTGCTCGTT
  GGGAAGGACGGTGCCGTCTACGTGCATCACATGCGGGCAGCCCGACTTTGCCGCTTGGCTAGCAGCACCA
  CAGAGTTCACACGACGGGGGCTCCAGCGCGATGCTGTCACCTATGAGGAGGATCTTGAACTTCCAGACCA
  GCGGATGTGTGGCACTAATGCACGGCATCTCTTTGACGTTATCGCGGCGGCGGCAGACGAACACAACCTG
  CTGACAGTCGGGGGCCTCTGCCAGACACATGCAGGAGTAAGCTGCAATCTGCTCGAAACCGTCGGGGATC
  CATGGACAGCCGTGCCTGCCGCAAGGATGACTTTGACCGTGCCTCAGGTGCAGTACCGATTGTGGCCCGA
  GGCCCGAAGAGATCTGCGGAGGCATCTTTATGCAGGGCACCCACTCGGTCCTTGGTTGGTCTGCGGCGTG
  CTGTCCCGGGAGAGAGAAACTCAAAAACCGAGCCCACCGATCAGGACTACTGTCGGGAACGTGCCCACTC
  CCGGTCCTCGGGAAGTTGAAATCGCTTGGGTGGTGCTGACTTTGGCCGGGCCACTTCTGGCATTTTGGCC
  TGATACAGGTAAGATATGCAGGCTCGCAAATTCATTCAGCACCCTGTGGAAAATGGGACCTCGGGCAATG
  AGAGGACATTGGACTTATAGTGCACCTGGCCGCCACCTGCCGGGTGATGCATGGCCCTTGTGTGAGCACG
  TGAGGCCTCAGTGGAAGGCATCTGAAGAAGAGTCTGTGCCTGGACTGGACGCTACGGAAGTGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101439 representing NP_043000
  Red=Cloning sites Green=Tags
  
  MSTRHTQRTGGRISVRRNEARLAHARARARFDWLLLARGRPSKLYGYASRHRGELIHLPWPPCWCLELHH
  DPYRDARSATVWGHRWGWPATHVRPRCVQDCALDSCLYVCCGYGEKLQPVGFVKSYVTNSQLDTLRVLLV
  GKDGAVYVHHMRAARLCRLASSTTEFTRRGLQRDAVTYEEDLELPDQRMCGTNARHLFDVIAAAADEHNL
  LTVGGLCQTHAGVSCNLLETVGDPWTAVPAARMTLTVPQVQYRLWPEARRDLRRHLYAGHPLGPWLVCGV
  LSRERETQKPSPPIRTTVGNVPTPGPREVEIAWVVLTLAGPLLAFWPDTGKICRLANSFSTLWKMGPRAM
  RGHWTYSAPGRHLPGDAWPLCEHVRPQWKASEEESVPGLDATEVK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1185 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_043000
• RefSeq ORF: 1185
• Locus ID: 1487974
• MW: 44.6 kDa