U28 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101376

Myc-DDK-tagged ORF clone of viral ORF for ribonucleotide reductase subunit 1 [Human herpesvirus 6], codon optimized for human cell expression, NP_042921

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U28
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101376 represents NCBI reference of NP_042921 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAGGAAGGAGCGCCGGATCAACAAGGACTACGGGTATAATCGGAAATGTGTGTGTCACTATGAAG
  CGAGCCAGAAGCGGTTCTGCTACTCCCAATACTCATGCGCCAGCGTGCTGTACGAACGAGTCCGCGATAT
  CGCCAAGATCATAGATCGGCTGGATTCTGGACTGGATGCCTGGTGCCTGAGAGACGCTATTATTAGCGTG
  CTGAGAGCGACACATTGCGTGCCTAGAGTGGACAGAATGCTGGGTCGATGGTACCTCAAGACAAGCGTGT
  TTTATGACTTTTGCCCTGACGACCTCATTCTGTCATGTCCTAACGTCATCATGCCGAACGTACTTAATTT
  TGTCAAGAAGTATCGGGATTTTATCCGGAGCGTATTGTACAAGGTAAGCGTGAGCTGGAAGAACCAGTAT
  ATGCCCGGAGTGCTGGCAGCGTCCAGGTTTCTGGAGGAAATTAGTAATTCTCTCAACGGTGTGGAAGAGT
  CCATTCCCTGCATCTACCTGAGGATGTGTGCTACACTGACAGAAATTGTTCTGCGAATCGGGTACCTCAG
  GGAGATTTACCAGGAGAATCCGTACGTAATGTTCGAGGAACTCGCTTTTTCCCTGTTTACCCAGAAGTGG
  GTCTTGCCGTTCTCCTGTATGACAAATTTGGGACTGGTAGAAAAAGCAAACTCCACCGTGTTTGACGTGG
  CCATCTATAACACCTGCCTTTACAGCCTCGTCGATTTCACCATTGTAAATGGCGAGCATCTGTTTCCCGC
  TCTCCTCAATGGCTCTAACATCTCAATGAACCTGACTCGATACCAACAGGAGGCCAAGAACATATTCGAG
  ATCCTGTTGAGCCAAATTAGGGTGGTCGAACGCGATACAGACAAGACCGTTCAACTGACCGTGTACGTCG
  AGGTCTGGCATGTAAGTGCACTGATGTGGCTTGATCTTTACGAGGCCCTGCCGCAGACAAGTCGGGTGAC
  GTTCTGTTTGATCATTCCTGGAATTTTCATGGACAGATACGAACTGAAGAGAGCCCAGTGGAGCCTCTTC
  CACAAGAACATTGCCTTCGAACTGGGAAAATGTGATGAGATTACATTCTCTACCAAGTATCTTGAATTCG
  AGCGAACCACCGATCATGCAAAGATCACAATGAGCTCATTCATCGAGAAGATTTGCCTTTGTCTCAAGGG
  AGGGCGGATGGGGCTCATTTTCCGCAAAAATGTGTATCAATACAGTATGATACCCCACGTGCCCCTGTAC
  TGCGGCGGAGATTTTCTTGATGTTCTCCCTGTGAGGGATGGGATCAACACCTGCATGCGCATGCTGCTGA
  ACGTCGTGCATTTCCTCGGTGACGAAGTCAGTGACGAGCTGACAGAAGAGATCGATTTCGTTAGGCTGCA
  GTGCAAGTTTTTTATGTTTAACGAACTGCGCAGAGTGGTGCGGAAGATGGTCTTGGTCGCAAACGCTGTA
  ATTGATTATGCGGTCGAAAACAAGGACTTCCTGTGCGAGGGCATTGAAGACGGCAGGAGTCTGGGAATCT
  GTGTCACCGGGCTGCATAGCGTGTTTATGACCGTGGGCCTTAGCTATGCGCATCCCGATGCACGGCGGCT
  CTACCGGATGATATGTGAACACATTTATTATACCTGCGTGAGAACGTCTGTTGATTGCTGCATGAAAGGA
  GCTGAACCTTGCAACCTCTTTGACAGATCCAAGTATGCGTTGGGAATGCTTTATTTCGATCACTTTGACA
  ACGTCGAATGCACCCTTCCTGAAGAACTTTGGACGACCCTCCGCAAAGATGTCCTCATGCATGGCGTACG
  CAACATCCACTTCACGGCCGGCACTGCAATGCAAAAGGAGTTCGACATTATTAATAGCAGCGAGAGCTTC
  TGGCCAATGGAGGACAACAAGATCCTCCGGAGGTCAAATATAAAGGTAGTTATCGGGAAGGATGGCCTCA
  ACGACGTGACTTCTGTATACTCCAGTGAGCTGAAGAGCCTGTACATCCCTGTGTACAATAACTTGCTTTT
  GAACCGGTTCAACAAGCACCAGCAGTATCTCAAAACCGTGGGCTATAGGGTGCTGAATGTGGATACCAAC
  TTGTTCACTGATAAGGAACTTGATGATCTCGCTGTGTTCAAAGACGGCTTCTCCTACCACCTCAATGATC
  TCATCGAGATGTACAAGAGCGGTCTGCCATTTCTGGACCAGGGCCAAGCAAATGTATTCTATTTTAACGA
  CACTGTGAGCCTGCGACTCCTCCTGCCCCTTCTCTATAAAGCCGGCTTTAAAGTCGCAATGTACAAGGTC
  TTGTGTAACAGTGAAATGTACAAGCACCTGGACCTCTCCAACCCCTTGCCCCTTATCGGTAAGTGTAGCG
  ACGGGGTGGTCATGCACGTAAAGAACATACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101376 representing NP_042921
  Red=Cloning sites Green=Tags
  
  MKRKERRINKDYGYNRKCVCHYEASQKRFCYSQYSCASVLYERVRDIAKIIDRLDSGLDAWCLRDAIISV
  LRATHCVPRVDRMLGRWYLKTSVFYDFCPDDLILSCPNVIMPNVLNFVKKYRDFIRSVLYKVSVSWKNQY
  MPGVLAASRFLEEISNSLNGVEESIPCIYLRMCATLTEIVLRIGYLREIYQENPYVMFEELAFSLFTQKW
  VLPFSCMTNLGLVEKANSTVFDVAIYNTCLYSLVDFTIVNGEHLFPALLNGSNISMNLTRYQQEAKNIFE
  ILLSQIRVVERDTDKTVQLTVYVEVWHVSALMWLDLYEALPQTSRVTFCLIIPGIFMDRYELKRAQWSLF
  HKNIAFELGKCDEITFSTKYLEFERTTDHAKITMSSFIEKICLCLKGGRMGLIFRKNVYQYSMIPHVPLY
  CGGDFLDVLPVRDGINTCMRMLLNVVHFLGDEVSDELTEEIDFVRLQCKFFMFNELRRVVRKMVLVANAV
  IDYAVENKDFLCEGIEDGRSLGICVTGLHSVFMTVGLSYAHPDARRLYRMICEHIYYTCVRTSVDCCMKG
  AEPCNLFDRSKYALGMLYFDHFDNVECTLPEELWTTLRKDVLMHGVRNIHFTAGTAMQKEFDIINSSESF
  WPMEDNKILRRSNIKVVIGKDGLNDVTSVYSSELKSLYIPVYNNLLLNRFNKHQQYLKTVGYRVLNVDTN
  LFTDKELDDLAVFKDGFSYHLNDLIEMYKSGLPFLDQGQANVFYFNDTVSLRLLLPLLYKAGFKVAMYKV
  LCNSEMYKHLDLSNPLPLIGKCSDGVVMHVKNIL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 2412 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042921
• RefSeq ORF: 2412
• Locus ID: 1487905
• MW: 93.4 kDa