U38 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101386

Myc-DDK-tagged ORF clone of viral ORF for DNA polymerase catalytic subunit [Human herpesvirus 6], codon optimized for human cell expression, NP_042931

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U38
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101386 represents NCBI reference of NP_042931 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCCGTAAGCTTCTTTAACCCATACCTGGAGGCAAACCGCTTGAAGAAGAAAAGCCGGTCCAGTT
  ACATCAGGATACTCCCAAGGGGCATCATGCACGATGGCGCCGCCGGACTTATCAAAGACGTGTGCGACTC
  AGAGCCTCGGATGTTCTACCGGGATAGACAGTATCTGCTGAGCAAGGAAATGACTTGGCCCAGCCTCGAT
  ATAGCTAGAAGTAAGGACTACGATCATATGAGGATGAAGTTTCATATTTATGACGCGGTGGAGACTCTCA
  TGTTTACAGACTCTATAGAGAATCTTCCTTTCCAGTACAGACATTTCGTTATCCCAAGCGGCACTGTGAT
  ACGAATGTTCGGCCGCACAGAGGACGGGGAGAAAATTTGCGTGAATGTGTTTGGGCAGGAGCAGTACTTT
  TACTGCGAATGCGTGGACGGGCGGTCTCTGAAAGCAACTATCAACAATTTGATGCTTACCGGAGAAGTTA
  AAATGTCTTGTTCCTTCGTTATTGAGCCCGCTGATAAACTGTCTCTCTACGGGTACAATGCCAATACCGT
  CGTGAATCTCTTCAAGGTTAGCTTCGGAAATTTCTATGTGTCTCAGAGGATTGGTAAGATCCTGCAAAAT
  GAGGGCTTCGTGGTATACGAGATCGACGTCGACGTGCTGACACGCTTCTTCGTCGATAACGGCTTTCTGA
  GCTTTGGCTGGTACAATGTCAAGAAGTATATCCCTCAGGACATGGGCAAGGGGTCTAATCTCGAGGTCGA
  GATCAATTGCCACGTGAGCGATCTGGTGAGCCTCGAAGACGTCAATTGGCCACTGTATGGTTGTTGGTCT
  TTCGATATCGAATGCCTGGGCCAGAATGGCAATTTCCCAGATGCAGAGAATCTGGGCGACATTGTTATCC
  AGATCTCCGTGATCTCTTTCGACACCGAGGGGGATCGCGACGAGAGACACCTCTTCACTCTTGGAACCTG
  TGAGAAGATAGACGGCGTGCACATCTACGAGTTTGCCTCCGAGTTCGAACTGCTCCTGGGTTTTTTTATT
  TTTCTCCGAATCGAATCTCCTGAGTTCATTACCGGATACAACATTAACAATTTTGATCTGAAGTACCTTT
  GTATCCGCATGGATAAAATTTATCACTATGACATTGGATGCTTCTCTAAGCTCAAGAATGGCAAGATCGG
  GATCTCCGTACCTCATGAACAGTACCGAAAAGGGTTCCTTCAAGCGCAAACCAAGGTGTTTACTTCCGGT
  GTGCTTTATCTGGATATGTACCCAGTCTATAGCTCAAAAATCACCGCCCAAAACTATAAGCTGGACACCA
  TCGCCAAGATTTGTCTCCAGCAGGAAAAAGAGCAACTCAGTTACAAAGAGATTCCTAAGAAATTTATAAG
  CGGTCCCAGCGGGCGCGCGGTGGTTGGCAAATACTGTCTGCAAGATAGCGTACTCGTGGTCCGACTGTTC
  AAGCAGATTAACTATCATTTTGAAGTGGCAGAGGTCGCTAGACTGGCCCATGTGACAGCCAGATGCGTGG
  TGTTCGAGGGGCAGCAAAAAAAAATTTTCCCGTGTATCCTGACCGAAGCCAAGCGCAGGAATATGATACT
  GCCGTCCATGGTTAGCAGCCACAATAGACAGGGAATAGGGTACAAGGGTGCCACTGTGCTGGAGCCCAAG
  ACAGGTTACTACGCAGTGCCCACGGTAGTGTTCGACTTCCAGTCCCTGTACCCCAGCATAATGATGGCCC
  ACAATCTTTGTTATTCCACCCTGGTCCTCGACGAGCGCCAGATCGCCGGACTGAGCGAAAGCGATATCCT
  GACCGTCAAGCTGGGCGATGAGACCCACCGGTTTGTCAAACCCTGTATTCGCGAAAGTGTGTTGGGATCC
  CTGCTCAAGGATTGGCTGGCCAAGCGCAGAGAGGTCAAGGCGGAGATGCAGAACTGTTCCGACCCCATGA
  TGAAACTCTTGCTGGATAAAAAACAACTGGCCCTGAAGACAACTTGTAACTCCGTGTACGGAGTCACCGG
  AGCAGCTCACGGATTGCTCCCATGCGTGGCAATCGCAGCGTCCGTCACCTGCCTGGGGAGGGAAATGCTG
  TGCTCTACTGTGGACTATGTAAATTCCAAAATGCAGAGCGAGCAGTTCTTCTGCGAGGAATTCGGCTTGA
  CCAGTTCAGACTTTACCGGGGATCTGGAAGTTGAGGTAATTTATGGAGATACCGACTCAATATTTATGTC
  CGTGAGGAATATGGTGAACCAGTCTCTCCGACGAATCGCCCCAATGATTGCAAAACACATCACCGATCGC
  CTTTTCAAGAGTCCGATCAAACTGGAATTCGAAAAAATTCTGTGTCCTCTGATTCTGATCTGCAAAAAGC
  GGTACATCGGAAGACAGGATGACTCTCTGCTCATCTTCAAGGGCGTGGACCTGGTCAGGAAAACCAGTTG
  TGACTTCGTGAAAGGAGTGGTAAAGGATATAGTCGACCTCCTGTTTTTTGATGAGGAGGTCCAAACAGCT
  GCTGTTGAGTTCAGCCATATGACACAGACCCAGCTCAGAGAGCAGGGGGTGCCGGTGGGAATCCATAAAA
  TCTTGCGCAGGCTCTGTGAAGCCAGGGAGGAGCTGTTTCAAAATAGGGCTGACGTCCGACACCTGATGCT
  CAGCTCTGTGTTGTCAAAAGAAATGGCCGCATACAAGCAGCCAAACTTGGCTCATCTTTCAGTGATCCGG
  CGCTTGGCGCAGCGAAAAGAAGAGATCCCAAACGTGGGCGATCGGATCATGTACGTACTTATTGCGCCAA
  GCATCGGCAATAAACAGACTCATAACTACGAACTGGCAGAAGATCCCAACTACGTGATAGAGCACAAGAT
  CCCAATCCACGCCGAGAAATACTTCGATCAGATAATCAAAGCTGTGACTAACGCCATTAGCCCGATCTTC
  CCGAAGACGGACATTAAAAAAGAAAAGCTCCTCCTGTACCTCTTGCCAATGAAAGTATACCTGGACGAGA
  CCTTCTCCGCTATTGCGGAAGTGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101386 representing NP_042931
  Red=Cloning sites Green=Tags
  
  MDSVSFFNPYLEANRLKKKSRSSYIRILPRGIMHDGAAGLIKDVCDSEPRMFYRDRQYLLSKEMTWPSLD
  IARSKDYDHMRMKFHIYDAVETLMFTDSIENLPFQYRHFVIPSGTVIRMFGRTEDGEKICVNVFGQEQYF
  YCECVDGRSLKATINNLMLTGEVKMSCSFVIEPADKLSLYGYNANTVVNLFKVSFGNFYVSQRIGKILQN
  EGFVVYEIDVDVLTRFFVDNGFLSFGWYNVKKYIPQDMGKGSNLEVEINCHVSDLVSLEDVNWPLYGCWS
  FDIECLGQNGNFPDAENLGDIVIQISVISFDTEGDRDERHLFTLGTCEKIDGVHIYEFASEFELLLGFFI
  FLRIESPEFITGYNINNFDLKYLCIRMDKIYHYDIGCFSKLKNGKIGISVPHEQYRKGFLQAQTKVFTSG
  VLYLDMYPVYSSKITAQNYKLDTIAKICLQQEKEQLSYKEIPKKFISGPSGRAVVGKYCLQDSVLVVRLF
  KQINYHFEVAEVARLAHVTARCVVFEGQQKKIFPCILTEAKRRNMILPSMVSSHNRQGIGYKGATVLEPK
  TGYYAVPTVVFDFQSLYPSIMMAHNLCYSTLVLDERQIAGLSESDILTVKLGDETHRFVKPCIRESVLGS
  LLKDWLAKRREVKAEMQNCSDPMMKLLLDKKQLALKTTCNSVYGVTGAAHGLLPCVAIAASVTCLGREML
  CSTVDYVNSKMQSEQFFCEEFGLTSSDFTGDLEVEVIYGDTDSIFMSVRNMVNQSLRRIAPMIAKHITDR
  LFKSPIKLEFEKILCPLILICKKRYIGRQDDSLLIFKGVDLVRKTSCDFVKGVVKDIVDLLFFDEEVQTA
  AVEFSHMTQTQLREQGVPVGIHKILRRLCEAREELFQNRADVRHLMLSSVLSKEMAAYKQPNLAHLSVIR
  RLAQRKEEIPNVGDRIMYVLIAPSIGNKQTHNYELAEDPNYVIEHKIPIHAEKYFDQIIKAVTNAISPIF
  PKTDIKKEKLLLYLLPMKVYLDETFSAIAEVM
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 3036 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042931
• RefSeq ORF: 3036
• Locus ID: 1487916
• MW: 115.8 kDa