U50 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101399

Myc-DDK-tagged ORF clone of viral ORF for DNA packaging tegument protein UL25 [Human herpesvirus 6], codon optimized for human cell expression, NP_042943

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U50
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101399 represents NCBI reference of NP_042943 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCAGTGCAACCTGTTTTATCAGTACCCAATTACCCCTATCCTGGAGGGACACGTACGCAATATTC
  TCATCTGCACAGAGAAGGATGTCGAAAAGCTCCAATCACAGTCTTCACTCAGACTCCGCGAGAAAATTGA
  TCAAGGTCACCGCGATAAGTTGCTGCGCATGCGGCTCAAAACAGAGCTCGATGCGTTGCAAAAAAAGATG
  CAAAAGGACTCAGACGTGCTGAACTCCCACCTTAAGGCCATTGAGGATGCACTCTTGTTCACAAACGACG
  GTGAAGTCAATGTCGAGACTAAGGCGGATGCACAGCTGATTCCTAAATCCCCTGAAAAACTGGAGAAGTT
  TAACCAGGTCGCGATTACACCCCTCGACCCCTTTATAAGGTTTACGGACGATTTCAGGGGAGAAATGATA
  AATACTTTCTTCAACAATGCTCAGATGTGGAATTTCACATTTGGTTCTTGGTTCTACAAACTGAAGCGAG
  TGTTTTATAACGAGCCTGGCCTGCGCCGGGCACTGAAGCTCACCAACGTAGATAGCCTTACCATCAGCAA
  GGAACTGTTGGCAGTGACTGTCAACGCACTCGAACAAGCAACAGTATACCCTATCTTCGGATCCGAAATG
  TCTGACCTTGAAGCTGCTCTCTGCATCCTCGCTGCATTCTATTCTACGTATGAAAACAGTCAAATTGATG
  AAAGGACTACACTGGTTGACGTCATCACACTCCTTCCTGTTATATTTCGCCTGCTTGGATCTGAAATTAC
  CGCTCTGAAAAATGTCTCCCCATCTGGAACCTACTTTGGATTTAACGATCCATCCTGTATGAAGTTCTTC
  GTCCCTATGAGGAAAGGAAAGCACTATGCAGAGAACACCTTCGGAAACCATGTGTTGATCAAAATGCTGC
  TCGGACGAGGTGTGATGCAAAAAATCCCTGGCGAGAAGATATCTCAGAATTTTGATGTGGAAGCGAGGCT
  GCACGGGGCCATCAAGAATGATGTCTTGGTCTATTGGACCTACCAGCTCATGAGACCCAAGTTGGGAAAT
  AATGTGCCTATATTTATCCATGACCAACACTACCTGCGGTCCGGGCTTGTGGCAATCGAGTCTCTTTTCT
  TGCTCTGGAGAATCCTTAACAGCGAGTCCCTGTTTAATAAGAGGGTTGGGAAGTTTCTGCTGACCTCAAT
  CTTTCCGCAGCTGGAGAACGTGGACTTTGCTGAGAATAACTTTGAGGCAGGCAATATCCAGAATTTCGAA
  TACCTGATGCACCATTATGTGGTCCCCATGTACAACCTGCAGAATGACATCAGCATCTCTACACTGTTTC
  CTGGTCTGGTCGCCGTCTGTGTGAACGAGAGTGTTCGACTCGGCTGGGAGCACAAATGTGCAGGGGCACC
  TAGCGACGCCGTCCAAGTGCAAAGTAAGGAAAATCCCTTTGTCGAGTATATACGCGCACAAATGGAGCAG
  CAAGCGGACGTTGCAATCCTGGAGAAGCACGACTGTATCCTGTTTCACCTGGAGAATGGCCTGAACATCA
  CTCTGAGCTTCACCCTGCCCCGACAAAGACTTTTTGCCATGGCGAGCTCACTGTTTAACGTTAATGACAC
  ATACGATTTCATATACTTCTTGGTTCTGGGCTTTTTGCCCATTCCGGCCGTGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101399 representing NP_042943
  Red=Cloning sites Green=Tags
  
  MAQCNLFYQYPITPILEGHVRNILICTEKDVEKLQSQSSLRLREKIDQGHRDKLLRMRLKTELDALQKKM
  QKDSDVLNSHLKAIEDALLFTNDGEVNVETKADAQLIPKSPEKLEKFNQVAITPLDPFIRFTDDFRGEMI
  NTFFNNAQMWNFTFGSWFYKLKRVFYNEPGLRRALKLTNVDSLTISKELLAVTVNALEQATVYPIFGSEM
  SDLEAALCILAAFYSTYENSQIDERTTLVDVITLLPVIFRLLGSEITALKNVSPSGTYFGFNDPSCMKFF
  VPMRKGKHYAENTFGNHVLIKMLLGRGVMQKIPGEKISQNFDVEARLHGAIKNDVLVYWTYQLMRPKLGN
  NVPIFIHDQHYLRSGLVAIESLFLLWRILNSESLFNKRVGKFLLTSIFPQLENVDFAENNFEAGNIQNFE
  YLMHHYVVPMYNLQNDISISTLFPGLVAVCVNESVRLGWEHKCAGAPSDAVQVQSKENPFVEYIRAQMEQ
  QADVAILEKHDCILFHLENGLNITLSFTLPRQRLFAMASSLFNVNDTYDFIYFLVLGFLPIPAVI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042943
• RefSeq ORF: 1665
• Locus ID: 1487930
• MW: 63.6 kDa