U90 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101433

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein IE1 [Human herpesvirus 6], codon optimized for human cell expression, NP_042983

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U90
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101433 represents NCBI reference of NP_042983 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGCCAAGCCAAGTGGAAATAACATGGGGAGTAATGACGAACGCATGCAGGATTACCGACCCG
  ATCCGATGATGGAAGAGTCCATCAAAGAGATACTGGAGGAAAGCTTGATGTGTGATACCAGTTTTGACGA
  CTTGATTATACCTGGGCTGGAGAGCTTCGGCCTCATCATTCCTGAGTCATCCAATAACATAGAAAGTAAC
  AATGTGGAGGAAGGGAGTGATGGAGAGTTGAAAACTCTGGCCGCCCAGTCTGCTGGCAATTGTATACAGT
  CCATTGGTGCCTCCGTCAAAGCGGCGATGAAACAAGAGCAGTCTGACATGGAGGACAAACTTATCAAGTG
  TGCCGGCTTGTTGACTCAGCAACAGAGTATGTTCATAGGTCTGGGGCTCGAACAACTGAGCCAGCTGATC
  AACATCAATCTGCTCAGCTCTGCCAGTACCAAATACGTTGAAAGCTATAGCAAGATGCTGCATGGAAAAG
  AGCTGGATTTCTTCAATTGGTGTGAGCCTCGCTTTATAGTCTTTGCTTGTGATAAATTCGACGGGTTGGT
  CAAGAAAGTGGCCTCCGAGTCTCGAAACCTCCTCCTCGACCTGAGGGCAAACATGAACAACGATATTATC
  AAGGCCGTGAAAGACATCTTCAGCAAAGCTACCGTCACCTTGGACTGTCAAAAACTTAACCAAGGAGCTA
  CCATGTTGATGATGATGGCACATAATAAGGAAATGAGTAATCCCGATATCAGTTCCAAAGACTTTTGCGA
  AAAAATTAATACGCTGAAGCAGACACTCCTGGAGGGAAAGAATGAGATTGTGGAGACCAACGCTAAGAAC
  ATGCAGATCCTCCAGACCTTCGCCATTAAACAAATGAATCAAATATTCATGGATGGATGTGATAAGGCCT
  TCTTGAAGTTGAACGTCAACTGTAAGAATTTGATCACCGCGGCCAAAAATTTGGCTAACACTATCCTCCA
  GTCAATTGTGATCTGCAGTAACGAGTTTTCTTGGCAACATCTCAAACTCCTGCGCCGGGGTTTTAAGGTT
  ACAATGCTGAACATGATTACCCAGGCCTGTGAATGTCTGGAATCCGATTACGACGATACCGGTCTGATAA
  AACCTTTGACTCCTCTGCAGATCATGGACGGATACATCAACATGAACAAAAACAGGCAAAGTTCCATCTG
  TGACGGAAATACTGACCCTAGCGATTCCATGATCCTTGACCTGGCTGACTTCGACGATCACGGACGCTAC
  AGTGAAGAGAGCAGCATTGAGTCAATCCATGAAGACGATGACAATAAAATGTACCCCTGCACCCCAAGCC
  CCGAGGTGCCCGGCAAATCTAAGTACGTAGGCACATTCACTGAGAACAGCCGGCAATCCGGAGATGAACA
  GACCAATCCAAACTGCGTCGGAACGGCCTCCGTGACAGACCTTGGAGGGCCTGACAATCTGAACAGTATA
  TCCGGCCTGCAGTCCTGTAAAAACATGCTCCTGGAGCGGCTGTTGGATACACAGTGTGATTCTGTTGTGG
  AAGGGACAGAACAGGATGGATCTTATGGTAACACTCTGATTAGCGAGATGATGATGTTCGGGTATGAGAC
  TGATCATAGTGCTCCCTACGAATCCGAGTCTGACAATAATGATGAAATCGACTACATCGCCAACTCAGAT
  TCTGCCGCAAGAACCAATAATATCCATATGAACTCTACCGACGAAAACACTCCATTCTCAAACTCAATCT
  GCTCCCCGCCCGAAGTAACGCCATCCAAGAAAAACGTCAAACCAAAGTCTATGACTCCCGGTTCCAAGCC
  CAAGAAGAGGGTGGCTAAAAGAAAACATGTCTCTTCAAAAAGCCCTAAAAATAAAAAAATAAAAACAGAT
  CAATTGCCAAAGGCGGCAGACGTGATTGTGATCAGCAGTGAGTCCGAAGATGAAGAGGACGGCGATAACA
  TTATCGGAAACTCTATCCTGATCAAAGCTATTAAGTCCGAGAGCGACAGTGAGAGTTCTTCTGAGAGTAA
  TGACTGTACAAGCGAGCACAAGCAGCTGCACTTGAGTGATTACGATGAGGTAACAAATAATGGCCACTGT
  CCCAGCTACGGGTTTCCTACTCCGGTCTTCACCATACCAATCCGAAGCATGCAGGGGACAGGGGGGATTA
  AATCTAAATTCATTCCCAAAAAGAATTGGATCTGGTATATGAAGAAGACACATCAAGTGGATAATTGTCC
  GATCCATAATTCTGAGAACGTTGATGCTAAAGATGACAGCGATGGCACTGAAGCAAAACACTGTTTTATG
  AACCACTTCGTCCCTATTAAAACTGACGATGAAGACTACGATAAGAATAATGTAAGTTACATCTACAACA
  AAATACAGAACAGCAAAATTGATTCAGGGGACATTATCCCTACCAAAAAGCTCATCATTGACATGGTTAT
  GGACAATTTTATGGATCTGAACGACATTATTAAACAAGGGATCACAAAACACTGTCAGGACCTTTGTAAT
  AAATATAACGTTGTCACACCAACCACATGCGAGGATGACCTGAACATGACTAACTCACAAACATTCGCCA
  CAACAGCAACCCAGGTTTTCGATCCTCCCGTGACAGGAAATAACTCTAGTATTCTGAATATTATTAATGA
  CACAACCTCTCAAAACGACGAGAACAGGTGCACAGAAGGAACCTCAAATTCCAACGAGAAATGTACCAAC
  ATCTCAGATTGTAATAGCGATGGCACCGAGGCCTTCAAACTTGACGGATACCCCAGCGACTATGATCCTT
  TTGTCGAGAATGCACAGATCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101433 representing NP_042983
  Red=Cloning sites Green=Tags
  
  MEPAKPSGNNMGSNDERMQDYRPDPMMEESIKEILEESLMCDTSFDDLIIPGLESFGLIIPESSNNIESN
  NVEEGSDGELKTLAAQSAGNCIQSIGASVKAAMKQEQSDMEDKLIKCAGLLTQQQSMFIGLGLEQLSQLI
  NINLLSSASTKYVESYSKMLHGKELDFFNWCEPRFIVFACDKFDGLVKKVASESRNLLLDLRANMNNDII
  KAVKDIFSKATVTLDCQKLNQGATMLMMMAHNKEMSNPDISSKDFCEKINTLKQTLLEGKNEIVETNAKN
  MQILQTFAIKQMNQIFMDGCDKAFLKLNVNCKNLITAAKNLANTILQSIVICSNEFSWQHLKLLRRGFKV
  TMLNMITQACECLESDYDDTGLIKPLTPLQIMDGYINMNKNRQSSICDGNTDPSDSMILDLADFDDHGRY
  SEESSIESIHEDDDNKMYPCTPSPEVPGKSKYVGTFTENSRQSGDEQTNPNCVGTASVTDLGGPDNLNSI
  SGLQSCKNMLLERLLDTQCDSVVEGTEQDGSYGNTLISEMMMFGYETDHSAPYESESDNNDEIDYIANSD
  SAARTNNIHMNSTDENTPFSNSICSPPEVTPSKKNVKPKSMTPGSKPKKRVAKRKHVSSKSPKNKKIKTD
  QLPKAADVIVISSESEDEEDGDNIIGNSILIKAIKSESDSESSSESNDCTSEHKQLHLSDYDEVTNNGHC
  PSYGFPTPVFTIPIRSMQGTGGIKSKFIPKKNWIWYMKKTHQVDNCPIHNSENVDAKDDSDGTEAKHCFM
  NHFVPIKTDDEDYDKNNVSYIYNKIQNSKIDSGDIIPTKKLIIDMVMDNFMDLNDIIKQGITKHCQDLCN
  KYNVVTPTTCEDDLNMTNSQTFATTATQVFDPPVTGNNSSILNIINDTTSQNDENRCTEGTSNSNEKCTN
  ISDCNSDGTEAFKLDGYPSDYDPFVENAQIY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 2823 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042983
• RefSeq ORF: 2823
• Locus ID: 1487968
• MW: 104.6 kDa