U43 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101391

Myc-DDK-tagged ORF clone of viral ORF for helicase-primase primase subunit [Human herpesvirus 6], codon optimized for human cell expression, NP_042936

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U43
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101391 represents NCBI reference of NP_042936 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACAATCGTTGTTTTTGCCACCGAGTACGATGCTGCTAATGTCATCTTTAGCATACTCTGCCGGTCCC
  CGTCCGAGCATCTGATCTTCCCAATTATAGTGAAATACAAACCAAGTAATAATGTCAGCTTCTGCCTGCA
  GACTCAGAAGTGTAAGAACAGCAAAAGGATTGATACCGTGTTCGTCTGCCACGCCGAAAAGCTGAATCTC
  TCACACTACATACAGACAGCATCACCAATCAAAGCCGAAGATGTCGCCAACAGTTTGAACGATAAAGAAA
  CCGAGTCACTGTACGTGGACATGATCCTCTCCCAAACCGGGAAAGAACGCGAGGATGTAGAGTTTAAATA
  TATGGCATACTTTCACAAAAGTCTGATCATCAAATACCTGACCGGAAAGTTTCTGCTGCCTACATCTCCA
  TTCTGGTTCCTCTCTACTTACGGGCAGACAGAGGGCATGCTGCTGTTGACCATGTTCTACTACCTGTTCG
  AGGAGCAGAAATCAACCATTACGACGACTAAGAATTATGTTCAATGTTTCACCGAGAACACAGGCAACAT
  GGTCTTCACATACTCTAGCATGTCCGAGTTTATCAATATAACTCTGAAAAGCAAGTTTAGGAAACTCTTT
  GCAGACTTTAGTACCTACGCCAGACAGAAAAACCTCCGGGACAAGGAGGAATTTAAGCATCTGGATACAC
  AGATTAACCTCTTCCGCAAGAGCTCCCATCTGACAAATACCTTTCGAGTGCACTATATTTATATAGCCTA
  CAATACCGCTCTGGAGACCACCAAATTTGTGAATTACTGCAACCTCACCTCTTACGATTCTAATCTCCCT
  ATTGGTCAGCAATGCCAGCGGAACCTGCATATCCTCGGTAATTCCCTCCACGAGAATCTGCTGTGTATCA
  TGAAACAGTATTTCAACGCAGATTGTTACTTCAAGACTTACATCGACATCAAGAGGCTGAAGAATCCTAA
  CCTCAATGTCACCGAATACGAATATGCCCTGGTGTCCAAAAAGAAAACTATCCAAGCCCTCACCAGCGAG
  CAAATCACTCGAGCAATAGCTAAATGTAACAAGAATGGGGAGGGTCTGTTTTCTCCAGTAAAGCCTGGGC
  TCCAGGGGCTGCTGGAGATATCTGCATCCGACAGATACGTTCAGATCCAGGACAAGAGAATCTACCGCCG
  CCAACACCTGCACAAGGACTATCACCGCCCATTCCCCGTGTTTAGAGTGCAGTTGCTGCACAAGAACATC
  TTTTGCTTTGGCAACTCAGAGGATTGGTATGAAAACATGGGCTTCAATAGAATATTGCAATATCTCCCGG
  ATGAATATATATCCGACGAGGCCCTGACGAGAGCCATCTGGCTGCAGGACACCCACTTCCTGTGTGATGA
  TGTGGAAAAGCAATTCTACACCACGCGGCACGAAATCTTCAACGAGAGGATCCCCGTGACTAACTACATC
  GGCGACCTCGACCTGCCCCTTCAGGACACCGCAACCATAACCGAAGAGACATTTTTTAGCATGTGTCGCC
  TGATTCGCCTCACCCTCATCAATGCCTGGAAGAAGATCTTCCCTTCAATCGATACAGACACTCATCCTAT
  CTTTTTCTTTAAGACACAGTGCGACACGACCAACGACACTCTCGACTATACCGAAGACCCCACAGAAATC
  AAGCAGTTTTGCGTATGTAGAAAGAAAATCGGGCTTCGAATCAGCATCCCATTGCCTAATGGAACAGCTA
  TTGCCGGCGGGGAACCTTTGAAACAGCTTTCCAAGATTCTTAACCATGTAATGTGCCTGGACCAGGAACT
  CAGCCAGATACTCAATTCTATCACCTTCCCCGGCGAATGCTTCGACATTGGCATCTATCACACAGGACAC
  TGCATACGGATTGGATATATGTACAAAACTGACATGGATAAGGGGAAGATGCTCCATGGACGGCTTACAC
  CAATCTTTATTGTCCCCGAAGGCTATAGGAACAGTTGCAAGACCTTTATACAAATGCAGATGGATCTCAA
  CAATCTCCTGCATCATGGCACAAAAAAGGCCCCCATTGAGGAGCTGATCTACAATATTACAGATAAGGGG
  TGCCCAAAGGAGAATCTTAGTTTTATGGACCTGAAGAGTAGGCAGTTGTGGAACAAGGTGAACATAGCCA
  CCGAAACACTCATAACGAAATACCTGAACACTCATGGCTTCAATAATAACGCCACAAGCGCTGACGACAG
  CTTGCTGTCCTTCATCCGCCTCATAGGCTGGCCTATAATAAAAACACAACTCATCACACACTACGAAACA
  AGAATCGCCCAGCAGTTCTCTCAGGTTACTTTTATAAAAATTGATTCCAAGAATCTGCAAATTAAAAAGA
  CCCAGTTTGGGCGCGTAAGCGACTTTTCCTGCCTCAATCGCCAACACAGAGGAAATCGAGATAATGTTCT
  GGTCTATATTCAGCTGAAGGCGGACGGAAACCGGCTGATTCTGATTCTCTGGAGCACCTGCTTTGCAACT
  AAATGCCAGTCTAACTCCAAGCAAGTTCATTGCAGCATAGCGCTGGAGCAGCTGAAGAAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101391 representing NP_042936
  Red=Cloning sites Green=Tags
  
  MTIVVFATEYDAANVIFSILCRSPSEHLIFPIIVKYKPSNNVSFCLQTQKCKNSKRIDTVFVCHAEKLNL
  SHYIQTASPIKAEDVANSLNDKETESLYVDMILSQTGKEREDVEFKYMAYFHKSLIIKYLTGKFLLPTSP
  FWFLSTYGQTEGMLLLTMFYYLFEEQKSTITTTKNYVQCFTENTGNMVFTYSSMSEFINITLKSKFRKLF
  ADFSTYARQKNLRDKEEFKHLDTQINLFRKSSHLTNTFRVHYIYIAYNTALETTKFVNYCNLTSYDSNLP
  IGQQCQRNLHILGNSLHENLLCIMKQYFNADCYFKTYIDIKRLKNPNLNVTEYEYALVSKKKTIQALTSE
  QITRAIAKCNKNGEGLFSPVKPGLQGLLEISASDRYVQIQDKRIYRRQHLHKDYHRPFPVFRVQLLHKNI
  FCFGNSEDWYENMGFNRILQYLPDEYISDEALTRAIWLQDTHFLCDDVEKQFYTTRHEIFNERIPVTNYI
  GDLDLPLQDTATITEETFFSMCRLIRLTLINAWKKIFPSIDTDTHPIFFFKTQCDTTNDTLDYTEDPTEI
  KQFCVCRKKIGLRISIPLPNGTAIAGGEPLKQLSKILNHVMCLDQELSQILNSITFPGECFDIGIYHTGH
  CIRIGYMYKTDMDKGKMLHGRLTPIFIVPEGYRNSCKTFIQMQMDLNNLLHHGTKKAPIEELIYNITDKG
  CPKENLSFMDLKSRQLWNKVNIATETLITKYLNTHGFNNNATSADDSLLSFIRLIGWPIIKTQLITHYET
  RIAQQFSQVTFIKIDSKNLQIKKTQFGRVSDFSCLNRQHRGNRDNVLVYIQLKADGNRLILILWSTCFAT
  KCQSNSKQVHCSIALEQLKN
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 2580 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042936
• RefSeq ORF: 2580
• Locus ID: 1487921
• MW: 100.1 kDa