U94 (NC_001664) Virus Tagged ORF Clone

CAT#: VC101435

Myc-DDK-tagged ORF clone of viral ORF for protein U94/rep [Human herpesvirus 6], codon optimized for human cell expression, NP_042987

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: U94
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101435 represents NCBI reference of NP_042987 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTCTATAATTAACCCCAGCGATGACTTTTGGACAAAAGATAAGTACATTATGCTTACTATCAAAG
  GCCCCATGGAGTGGGAGGCAGAAATTCCTGGCATCTCTACTGACTTTTTTTGCAAGTTCTCAAATGTCAG
  TGTTCCCCACTTCCGCGACATGCATTCACCAGGCGCACCCGACATTAAGTGGATTACTGCATGTACGAAG
  ATGATCGACGTTATTTTGAATTACTGGAATAATAAGACCGCAGTGCCTACACCTGCCAAGTGGTATGCTC
  AGGCGGAAAACAAAGCGGGTAGACCTAGCCTGATTCTGCTGATTGCTCTCGATGGTATACCCTCAGCAAC
  GATCGGAAAACACACAACAGAGATTAGAGGCGTGCTTATTAAGGATTTCTTCGACGGAAATGCTCCGAAG
  ATAGACGACTGGTGCACCTATGCGAAGACAAAAAAAAATGGCGGAGGGACACAGGTCTTCAGCCTGAGCT
  ATATTCCTTTTGCTCTGCTGCAGATCATCCGGCCACAGTTCCAATGGGCTTGGACCAATATTAACGAACT
  GGGAGATGTGTGCGATGAAATTCATCGCAAGCACATAATTAGCCACTTCAATAAAAAACCTAATGTGAAA
  CTGATGCTGTTTCCAAAGGATGGCATTAATGGCATCAGCTTGAAGTCCAAGTTTCTGGGCACCATCGAGT
  GGCTCTCCGACCTGGGAATCGTGACTGAGGACGCCTGGATAAGAAGGGACATTAGAAGTTACATGCAGCT
  GCTCACTTTGACTCACGGGGACGTCCTTATCCATCGCGCTCTTAGCATTGCCAAAAAGCGCATCCGCGCC
  ACCCGCAAAGCCATTGACTTTATTGCCCACATTGATACCGACTTTCAAATTTACGAAAACCCTGTTTACC
  AGCTGTTCTGTTTGCAATCCTTCGATCCTATCCTGGCGGGTACCATCCTCTACCAGTGGCTCTCTCACCG
  CGGAGGAAAAAAGAACACTGTCAGTTTTATAGGCCCCCCTGGCTGCGGCAAGAGTATGCTGACAGGCGCT
  ATCCTCGAAAACATTCCTCTCCACGGAATCCTCCACGGCTCCCTGAATACCAAGAACCTGAGAGCTTACG
  GCCAGGTCTTGGTGCTCTGGTGGAAAGATATTTCCATAAATTTTGATAATTTCAACATAATTAAGTCACT
  CTTGGGCGGCCAAAAAATAATATTCCCCATCAACGAAAACGACCACGTCCAGATAGGCCCATGTCCCATT
  ATTGCCACGAGCTGCGTGGACATAAGGTCTATGGTCCATTCTAACTTGCATAAGATTAATCTGTCCCAGC
  GGGTGTACAATTTCACCTTCGATAAGGTAATCCCCCGCAATTTTCCCGTGATTCAGAAAGATGACATTAA
  TCAGTTTCTGTTTTGGGCACGAAATCGCTCTATAAACTGTTTCATAGATTACACAGTACCCAAGATACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101435 representing NP_042987
  Red=Cloning sites Green=Tags
  
  MFSIINPSDDFWTKDKYIMLTIKGPMEWEAEIPGISTDFFCKFSNVSVPHFRDMHSPGAPDIKWITACTK
  MIDVILNYWNNKTAVPTPAKWYAQAENKAGRPSLILLIALDGIPSATIGKHTTEIRGVLIKDFFDGNAPK
  IDDWCTYAKTKKNGGGTQVFSLSYIPFALLQIIRPQFQWAWTNINELGDVCDEIHRKHIISHFNKKPNVK
  LMLFPKDGINGISLKSKFLGTIEWLSDLGIVTEDAWIRRDIRSYMQLLTLTHGDVLIHRALSIAKKRIRA
  TRKAIDFIAHIDTDFQIYENPVYQLFCLQSFDPILAGTILYQWLSHRGGKKNTVSFIGPPGCGKSMLTGA
  ILENIPLHGILHGSLNTKNLRAYGQVLVLWWKDISINFDNFNIIKSLLGGQKIIFPINENDHVQIGPCPI
  IATSCVDIRSMVHSNLHKINLSQRVYNFTFDKVIPRNFPVIQKDDINQFLFWARNRSINCFIDYTVPKIL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001664
• ORF Size: 1470 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001664.2, NP_042987
• RefSeq ORF: 1470
• Locus ID: 1487970
• MW: 55.8 kDa