RL1 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101187

Myc-DDK-tagged ORF clone of viral ORF for protein RL1 [Human herpesvirus 5], codon optimized for human cell expression, YP_081455

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101187 represents NCBI reference of YP_081455 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGCCACTGACACTAACTCCACCCACACAACCCCCCTGCACCCTGAAGACCAGCATACTTTGCCAC
  TCCACCATAGCACCACCCAGCCACATGTACAAACCTCAGATAAACACGCTGATAAGCAGCATCGGACTCA
  GATGGAGCTCGACGCCGCTGACTACGCGGCGTGTGCTCAGGCGAGGCAACATCTCTATGGCCAAACCCAG
  CCTCAGTTGCATGCATACCCAAACGCAAATCCACAAGAGAGCGCCCACTTTCGCACCGAAAACCAACACC
  AGCTGACAAACTTGCTTCATAACATCGGGGAAGGGGCTGCCCTGGGTTATCCCGTACCCCGAGCCGAGAT
  CCGGAGGGGTGGCGGTGATTGGGCCGATTCCGCTAGCGACTTTGACGCCGACTGCTGGTGCATGTGGGGC
  CGGTTTGGAACGATGGGACGACAACCCGTTGTTACCCTCCTGCTGGCCAGGCAGAGAGACGGCCTGGCTG
  ACTGGAACGTTGTTCGATGCCGGGGAACAGGATTCCGCGCCCACGACAGCGAGGATGGAGTATCAGTGTG
  GCGCCAGCACTTGGTGTTTCTGCTTGGAGGGCATGGTAGGAGAGTACAGCTCGAGCGCCCGTCTGCCGGC
  GAGGCTCAAGCCCGGGGTCTGCTTCCCCGCATAAGAATCACGCCAATCAGCACGTCCCCAAGGCCCAAAC
  CACCACAGCCCACTACAAGCACAGCCTCACACCCCCATGCCACAGCCAGGCCTGATCATACACTCTTCCC
  TGTGCCGAGCACACCTTCAGCTACCGTGCACAACCCCAGGAATTACGCCGTGCAGTTGCATGCTGAAACA
  ACAAGGACATGGCGCTGGGCCAGACGGGGTGAGCGAGGCGCCTGGATGCCCGCTGAGACCTTTACCTGCC
  CGAAGGATAAACGGCCCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101187 representing YP_081455
  Red=Cloning sites Green=Tags
  
  MPATDTNSTHTTPLHPEDQHTLPLHHSTTQPHVQTSDKHADKQHRTQMELDAADYAACAQARQHLYGQTQ
  PQLHAYPNANPQESAHFRTENQHQLTNLLHNIGEGAALGYPVPRAEIRRGGGDWADSASDFDADCWCMWG
  RFGTMGRQPVVTLLLARQRDGLADWNVVRCRGTGFRAHDSEDGVSVWRQHLVFLLGGHGRRVQLERPSAG
  EAQARGLLPRIRITPISTSPRPKPPQPTTSTASHPHATARPDHTLFPVPSTPSATVHNPRNYAVQLHAET
  TRTWRWARRGERGAWMPAETFTCPKDKRPW
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 930 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081455
• RefSeq ORF: 930
• Locus ID: 3077430
• MW: 34.7 kDa