RL10 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101190

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein RL10 [Human herpesvirus 5], codon optimized for human cell expression, YP_081458

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RL10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101190 represents NCBI reference of YP_081458 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTATCCTAGAGTCATGCACGCTGTGTGTTTCCTGGCTCTGGGTCTTATCTCTTATGTGGCTGTATGTG
  CGGAAAATACAGTGACAACAAATTGCCTCGTCAAAGCTGAGAACACTCATCTCACTTGCAAGTGCAACCC
  TAACACCACCTCCAACACCAATAACGGCTCCAAATGTCATGCTATGTGCAAATGCAGGGTAACAGAACCA
  ATCACTATGCTGGGAGCATATTCTGCCTGGGGCGCCGGCTCTTTTGTGGCTACTCTGATCGTGCTGCTTG
  TGGTATTCTTTGTTATCTACGCCCGCGAAGAGGAAAAAAACAATACTGGTACCGAAGTTGATCAGTGCCT
  CGCCTATAGAAGCTTGACACGCAAGAAGCTGGAGCAGCATGCAGCTAAAAAGCAAAATATCTACGAGCGC
  ATACCTTATCGGCCCAGCCGACAGAATGACAATAGTCCCCTCATTGAACCGACTGGCACCGACGACGAGG
  AGGACGAAGATGACGACGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101190 representing YP_081458
  Red=Cloning sites Green=Tags
  
  MYPRVMHAVCFLALGLISYVAVCAENTVTTNCLVKAENTHLTCKCNPNTTSNTNNGSKCHAMCKCRVTEP
  ITMLGAYSAWGAGSFVATLIVLLVVFFVIYAREEEKNNTGTEVDQCLAYRSLTRKKLEQHAAKKQNIYER
  IPYRPSRQNDNSPLIEPTGTDDEEDEDDDV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 510 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081458
• RefSeq ORF: 510
• Locus ID: 3077518
• MW: 19.0 kDa