UL32 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101222

Myc-DDK-tagged ORF clone of viral ORF for tegument protein pp150 [Human herpesvirus 5], codon optimized for human cell expression, YP_081491

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101222 represents NCBI reference of YP_081491 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCTCCAGTTTATCGGCTTGCAGAGAAGAGATGTGGTAGCTCTGGTGAATTTTCTCAGGCACCTTA
  CCCAAAAGCCCGACGTGGATCTCGAGGCGCATCCAAAGATCTTGAAAAAGTGTGGGGAAAAGCGCCTTCA
  TCGCCGGACAGTTTTGTTTAATGAGCTTATGTTGTGGCTTGGCTACTATAGGGAGCTGAGGTTTCATAAT
  CCAGACCTTTCCTCCGTGCTCGAGGAATTCGAAGTGAGGTGTGCTGCTGTAGCCAGGCGGGGATACACTT
  ACCCTTTTGGAGACAGGGGTAAAGCACGCGACCACCTTGCAGTGCTGGATCGCACCGAATTCGACACGGA
  TGTGCGCCACGATGCCGAGATTGTCGAGCGGGCTCTCGTCTCTGCCGTGATACTGGCCAAGATGTCTGTG
  CGGGAAACTCTCGTTACAGCTATCGGTCAGACCGAGCCCATCGCGTTTGTACACCTGAAAGACACTGAAG
  TTCAGAGAATTGAGGAGAACCTGGAGGGGGTTCGGAGAAACATGTTTTGCGCTAAACCCCTCGATCTTAA
  CCTCGACCGGCACGCTAACACTGCCCTGGTGAATGCAGTGAACAAACTGGTTTACACAGGACGACTGATA
  ATGAACGTCCGGAGGAGCTGGGAAGAGCTCGAGAGAAAGTGCCTGGCACGCATACAGGAACGCTGTAAGC
  TTCTGGTGAAGGAGTTGCGCATGTGCCTTAGTTTCGATTCCAACTACTGCCGCAACATTTTGAAACACGC
  CGTGGAGAACGGAGACTCTGCAGACACCCTGCTGGAACTGCTGATCGAAGACTTTGACATCTACGTCGAC
  TCCTTCCCTCAGTCTGCCCATACATTTCTGGGTGCACGGAGCCCAAGCCTTGAATTCGACGACGACGCTA
  ACCTCCTCTCCCTCGGGGGCGGCAGTGCCTTCAGTTCTGTGCCAAAAAAACACGTTCCCACACAGCCTCT
  GGATGGCTGGTCCTGGATTGCATCCCCTTGGAAGGGCCATAAACCTTTTAGATTCGAAGCACACGGCAGC
  CTGGCCCCTGCAGCCGAGGCCCACGCCGCGAGATCAGCCGCGGTAGGTTATTACGATGAAGAAGAAAAAA
  GGAGGGAACGACAGAAAAGGGTGGACGATGAGGTGGTGCAGCGCGAGAAGCAACAACTCAAGGCCTGGGA
  GGAGCGACAGCAGAATCTGCAGCAGCGACAGCAGCAGCCACCTCCTCCGACACGGAAACCCGGAGCTAGT
  AGACGCTTGTTTGGCTCTAGTGCCGATGAAGACGATGATGATGATGACGACGATGAAAAAAATATTTTCA
  CCCCCATTAAGAAGCCAGGAACCTCTGGCAAGGGCGCAGCTAGCGGCGGAGGGGTGAGTTCAATATTTTC
  CGGGCTGCTGTCCTCAGGCTCTCAAAAACCAACAAGCGGCCCCCTGAATATTCCCCAGCAACAGCAGAGA
  CACGCCGCCTTCAGTCTGGTGTCCCCTCAAGTAACGAAAGCCAGCCCAGGTAGGGTGCGCCGCGACTCAG
  CGTGGGACGTGAGACCTCTGACTGAGACCCGGGGTGACCTGTTCAGCGGCGATGAAGATAGTGATTCCAG
  CGACGGATATCCCCCCAATAGACAGGACCCCCGATTCACTGATACCCTCGTGGACATTACAGATACAGAG
  ACTTCTGCTAAGCCTCCCGTGACCACTGCCTATAAATTCGAACAGCCAACCCTGACCTTCGGGGCAGGAG
  TGAACGTCCCTGCAGGCGCAGGGGCGGCCATTCTGACCCCTACGCCCGTCAATCCTTCTACCGCTCCAGC
  ACCGGCGCCTACCCCAACCTTTGCAGGCACTCAGACCCCCGTGAATGGCAATAGCCCCTGGGCACCTACA
  GCTCCCCTGCCGGGCGACATGAACCCCGCCAATTGGCCCCGCGAGAGGGCCTGGGCTCTCAAAAATCCAC
  ACTTGGCATATAACCCATTCCGGATGCCCACCACCAGTACCGCATCTCAGAACACAGTTTCCACCACCCC
  ACGCAGGCCAAGCACCCCCAGAGCTGCTGTCACCCAGACAGCAAGCAGGGACGCAGCAGACGAAGTGTGG
  GCTCTTAGGGATCAGACCGCCGAGTCTCCAGTGGAGGATAGTGAAGAGGAGGATGATGACTCTAGCGACA
  CCGGCTCTGTGGTATCCTTGGGTCACACCACTCCAAGCTCTGATTACAATAATGACGTTATTTCCCCTCC
  GTCCCAGACGCCAGAACAGTCAACCCCCTCCCGGATCCGAAAAGCCAAGCTCTCTAGCCCAATGACGACC
  ACAAGCACGAGCCAAAAGCCAGTCCTCGGAAAGCGCGTCGCAACACCGCATGCTTCAGCCCGCGCGCAAA
  CAGTCACTTCAACACCAGTCCAGGGGCGACTCGAAAAACAGGTGAGCGGCACTCCTTCTACCGTACCGGC
  GACGTTGCTGCAGCCACAACCAGCCAGCTCAAAGACTACTTCATCCCGGAATGTGACCTCAGGTGCCGGT
  ACTAGTAGCGCATCCTCCGCCCGGCAGCCAAGCGCATCAGCGAGCGTGCTCAGTCCCACCGAAGATGATG
  TTGTGAGTCCCGCTACGTCACCACTCAGCATGCTCTCCTCCGCCAGCCCCAGCCCTGCCAAATCTGCCCC
  GCCATCACCCGTGAAGGGGCGCGGCTCAAGGGTAGGGGTACCTTCTCTGAAGCCAACCCTGGGAGGCAAA
  GCAGTCGTCGGCCGACCTCCTTCTGTGCCCGTCAGTGGTAGCGCGCCTGGAAGACTGAGTGGATCTTCAC
  GCGCCGCAAGCACTACACCCACCTACCCAGCAGTTACCACTGTTTACCCTCCTAGTTCTACAGCTAAAAG
  TTCCGTGTCCAACGCTCCACCAGTGGCTTCACCATCTATCCTGAAGCCTGGAGCTTCCGCAGCGCTGCAG
  AGTCGGCGATCTACCGGCATAGCCGCCGTGGGGTCCCCAGTAAAGTCCACGACGGGGATGAAGACGGTCG
  CCTTCGACTTGTCAAGCCCCCAGAAGTCCGGGACCGGCCCCCAGCCCGGTAGTGCCGGTATGGGCGGGGC
  CAAAACCCCCTCCGATGCGGTGCAAAATATTCTTCAGAAAATCGAAAAGATCAAGAACACAGAGGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101222 representing YP_081491
  Red=Cloning sites Green=Tags
  
  MSLQFIGLQRRDVVALVNFLRHLTQKPDVDLEAHPKILKKCGEKRLHRRTVLFNELMLWLGYYRELRFHN
  PDLSSVLEEFEVRCAAVARRGYTYPFGDRGKARDHLAVLDRTEFDTDVRHDAEIVERALVSAVILAKMSV
  RETLVTAIGQTEPIAFVHLKDTEVQRIEENLEGVRRNMFCAKPLDLNLDRHANTALVNAVNKLVYTGRLI
  MNVRRSWEELERKCLARIQERCKLLVKELRMCLSFDSNYCRNILKHAVENGDSADTLLELLIEDFDIYVD
  SFPQSAHTFLGARSPSLEFDDDANLLSLGGGSAFSSVPKKHVPTQPLDGWSWIASPWKGHKPFRFEAHGS
  LAPAAEAHAARSAAVGYYDEEEKRRERQKRVDDEVVQREKQQLKAWEERQQNLQQRQQQPPPPTRKPGAS
  RRLFGSSADEDDDDDDDDEKNIFTPIKKPGTSGKGAASGGGVSSIFSGLLSSGSQKPTSGPLNIPQQQQR
  HAAFSLVSPQVTKASPGRVRRDSAWDVRPLTETRGDLFSGDEDSDSSDGYPPNRQDPRFTDTLVDITDTE
  TSAKPPVTTAYKFEQPTLTFGAGVNVPAGAGAAILTPTPVNPSTAPAPAPTPTFAGTQTPVNGNSPWAPT
  APLPGDMNPANWPRERAWALKNPHLAYNPFRMPTTSTASQNTVSTTPRRPSTPRAAVTQTASRDAADEVW
  ALRDQTAESPVEDSEEEDDDSSDTGSVVSLGHTTPSSDYNNDVISPPSQTPEQSTPSRIRKAKLSSPMTT
  TSTSQKPVLGKRVATPHASARAQTVTSTPVQGRLEKQVSGTPSTVPATLLQPQPASSKTTSSRNVTSGAG
  TSSASSARQPSASASVLSPTEDDVVSPATSPLSMLSSASPSPAKSAPPSPVKGRGSRVGVPSLKPTLGGK
  AVVGRPPSVPVSGSAPGRLSGSSRAASTTPTYPAVTTVYPPSSTAKSSVSNAPPVASPSILKPGASAALQ
  SRRSTGIAAVGSPVKSTTGMKTVAFDLSSPQKSGTGPQPGSAGMGGAKTPSDAVQNILQKIEKIKNTEE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 3147 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081491
• RefSeq ORF: 3147
• Locus ID: 3077422
• MW: 112.8 kDa