RL12 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101192

Myc-DDK-tagged ORF clone of viral ORF for membrane protein RL12 [Human herpesvirus 5], codon optimized for human cell expression, YP_081460

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RL12
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101192 represents NCBI reference of YP_081460 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCGTGGATAGACAGAAGAGAAATAATTTTACCTATAGGCAGACTGTTTATATCATTTTGACGTTTT
  ACATCGTGTACAGGGGAAAATGTAACTCAACTGACACAAATAACTCTACCTCAACCTCCGACAGTACAGT
  GTCAGACACAAGTGTGTACTCCACACCCAACCTGCCTTCAACTTTCTTCACCACCCTGGATACTTCTACT
  GGGAGTCAAATTAGTACTACATCCAACACAATTTCCAGCACTACTAACACACTCGCCGCTTCATCAATTA
  CAACACTGAACACGAGCACGAATACGACATCCTATACAGCCAGTACACTCACCGCGCTGAGCTCAACGCA
  TACCAATAGCCCCATCCTGTCAAACAATGCCACACTCTATACCACCAGTTTGGATAACACCACCACAGAC
  ATCACTTCCAGCGAAAGCTCTATCAATGCCTCCACTATCCATAATACCACATACAGCCCTGTGACTTCTA
  TAGCAGTGAACTGCACCGTGGCTGCCAACGAGACGAACAATAGTAGCTCTAAGAATTGCCAACAGGACAT
  AGGTACTATCAGGGTTAAGAGCACAACCCTGACGGCCGAAGAGGGCAAGAACATTACAATCCAGGGGAAC
  TCTACATGGAATTGCCCTAACGTCGTGTGGTACAGGCATTATAATTGGTCTACGCATGGGCATCACATAT
  ACCCGAATAGGCGCTACCAAACCCCGACGTATCAGCATAAGATCCTCACAAGTCACCCTATTTGTCACCC
  CGATGTATCAAGTCCAGCAGCCTATCACGATCTGTGCAGGTCCTGTAATAAAACCGAGCTCCGGATTTAC
  GACTTGAATACCACTAACTCCGGAAGATATAGTCGCAGGTGCTATAAAGAGTATAACCATGATGGCCCCC
  ACGAGGATGAAAACTTTGGATTGACTGTAAACCCTAGAAATGACACTGATAACCATACCACACCCCTCTG
  CCCCCGCTATGTCGGCACACAATCAGAGGAGGATGAGGACGATGATTACACCCTGAGCACCATAACCAAT
  AACAATATGCGGAAGACTAGTCATAGAGATATTTCTCACGGAACAAGAACCACCTGGGCTCTGACACTCA
  TCTGCATCGCCTGTATCCTCCTTTTCTTCGTCAGACGAGCGCTGAATAAGCGATACAGACCCCTTCGGGA
  CGATATAAGCGAGAGCAGCTTTGTCGTGCGATATCATCCAGAACACGAAGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101192 representing YP_081460
  Red=Cloning sites Green=Tags
  
  MRVDRQKRNNFTYRQTVYIILTFYIVYRGKCNSTDTNNSTSTSDSTVSDTSVYSTPNLPSTFFTTLDTST
  GSQISTTSNTISSTTNTLAASSITTLNTSTNTTSYTASTLTALSSTHTNSPILSNNATLYTTSLDNTTTD
  ITSSESSINASTIHNTTYSPVTSIAVNCTVAANETNNSSSKNCQQDIGTIRVKSTTLTAEEGKNITIQGN
  STWNCPNVVWYRHYNWSTHGHHIYPNRRYQTPTYQHKILTSHPICHPDVSSPAAYHDLCRSCNKTELRIY
  DLNTTNSGRYSRRCYKEYNHDGPHEDENFGLTVNPRNDTDNHTTPLCPRYVGTQSEEDEDDDYTLSTITN
  NNMRKTSHRDISHGTRTTWALTLICIACILLFFVRRALNKRYRPLRDDISESSFVVRYHPEHED
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 1242 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081460
• RefSeq ORF: 1242
• Locus ID: 3077488
• MW: 46.6 kDa