UL123 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101294

Myc-DDK-tagged ORF clone of viral ORF for regulatory protein IE1 [Human herpesvirus 5], codon optimized for human cell expression, YP_081562

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL123
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101294 represents NCBI reference of YP_081562 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGCAGCGCCAAGCGGAAGATGGATCCAGACAACCCAGACGAGGGACCCAGCAGCAAGGTCCCCC
  GGCCCGAGACCCCTGTGACTAAGGCCACCACTTTCCTGCAAACCATGCTCCGGAAAGAGGTCAACTCCCA
  GCTGAGCCTGGGAGACCCCCTGTTCCCCGAGCTCGCCGAGGAGAGCCTGAAGACATTCGAGCAAGTGACC
  GAGGACTGCAATGAGAACCCCGAGAAGGATGTCCTGACCGAGCTCGTGAAGCAAATCAAAGTGCGGGTGG
  ACATGGTGCGGCACCGGATCAAGGAGCACATGCTGAAAAAGTACACCCAGACCGAGGAGAAATTCACCGG
  CGCCTTCAACATGATGGGCGGCTGCCTGCAGAACGCCCTGGATATCCTGGATAAGGTGCATGAGCCTTTC
  GAGGATATGAAGTGCATCGGCCTCACCATGCAGTCCATGTACGAGAACTACATTGTGCCCGAGGACAAAC
  GGGAAATGTGGATGGCATGCATTAAGGAGCTGCACGACGTCAGCAAGGGCGCCGCAAATAAGCTCGGAGG
  AGCCCTGCAGGCCAAGGCCCGGGCCAAGAAAGACGAACTGAGACGGAAGATGATGTACATGTGTTACCGG
  AATATCGAGTTTTTCACCAAGAACAGCGCCTTCCCCAAAACCACCAACGGCTGTAGCCAGGCAATGGCCG
  CCCTCCAGAACCTGCCCCAGTGCAGCCCCGACGAGATCATGAGCTACGCCCAGAAGATCTTCAAGATCCT
  CGACGAAGAGCGGGACAAGGTGCTCACCCACATCGACCACATCTTCATGGACATCCTGACCACCTGCGTG
  GAGACCATGTGCAACGAGTACAAGGTGACCAGCGACGCCTGCATGATGACCATGTACGGCGGGATCAGCC
  TGCTCTCCGAGTTCTGCCGGGTGCTGTGCTGCTACGTGCTCGAGGAAACCAGCGTGATGCTCGCCAAGCG
  GCCCCTGATCACCAAGCCCGAGGTCATCAGCGTGATGAAGCGGCGGATCGAGGAGATCTGCATGAAGGTG
  TTCGCCCAGTACATCCTCGGCGCCGACCCACTGCGGGTCTGCAGCCCCAGCGTGGACGACCTCAGAGCCA
  TCGCCGAGGAGTCCGACGAAGAAGAGGCCATCGTGGCATACACCCTGGCCACCGCTGGCGCTTCCAGCTC
  CGACAGCCTGGTGAGCCCCCCCGAAAGCCCCGTGCCCGCCACCATCCCCCTGAGCAGCGTGATCGTGGCT
  GAGAACAGCGACCAGGAGGAGAGCGAGCAAAGCGACGAGGAACAGGAGGAGGGCGCCCAGGAGGAGCGGG
  AAGACACCGTGTCCGTGAAGTCCGAGCCCGTCAGCGAGATCGAAGAGGTCGCCAGCGAGGAGGAGGAGGA
  CGGCGCCGAGGAGCCCACTGCCTCCGGCGGCAAAAGCACCCACCCAATGGTGACAAGATCCAAAGCTGAC
  CAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101294 representing YP_081562
  Red=Cloning sites Green=Tags
  
  MESSAKRKMDPDNPDEGPSSKVPRPETPVTKATTFLQTMLRKEVNSQLSLGDPLFPELAEESLKTFEQVT
  EDCNENPEKDVLTELVKQIKVRVDMVRHRIKEHMLKKYTQTEEKFTGAFNMMGGCLQNALDILDKVHEPF
  EDMKCIGLTMQSMYENYIVPEDKREMWMACIKELHDVSKGAANKLGGALQAKARAKKDELRRKMMYMCYR
  NIEFFTKNSAFPKTTNGCSQAMAALQNLPQCSPDEIMSYAQKIFKILDEERDKVLTHIDHIFMDILTTCV
  ETMCNEYKVTSDACMMTMYGGISLLSEFCRVLCCYVLEETSVMLAKRPLITKPEVISVMKRRIEEICMKV
  FAQYILGADPLRVCSPSVDDLRAIAEESDEEEAIVAYTLATAGASSSDSLVSPPESPVPATIPLSSVIVA
  ENSDQEESEQSDEEQEEGAQEEREDTVSVKSEPVSEIEEVASEEEEDGAEEPTASGGKSTHPMVTRSKAD
  Q
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 1473 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081562
• RefSeq ORF: 1473
• Locus ID: 3077513
• MW: 55.1 kDa