US7 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101324

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein US7 [Human herpesvirus 5], codon optimized for human cell expression, YP_081592

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101324 represents NCBI reference of YP_081592 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGATCCAGCTGCTGCTGGTGAGCACACTGGTGGCCAGCATCGTGGCCACCCGGGTGGAGGACATGG
  CCACCTTCCGGACCGAGAAGCAGTGGCAGCAGGATCTGCAGTACCGGCGGGAGTTCGTGAAGAGACAGCT
  GGCCCCCAAACCCAAGAGCAACATCGTGGTGAGCCATACCGTCTCCTGCGTGATCGACGGCGGCAACATG
  ACCAGCGTGTGGAGATTCGAGGGCCAGTTCAACCCCCACATCGCCAGCGAGGTGATCCTGCACGACACCA
  GCGGCCTGTACAACGTGCCCCACGAGGTCCAGAACGACGGCCAGGTGCTCACCGTGACCGTCAAGCGGAG
  CGCCCCCGCCGACATCGCCAAGGTGCTGATCAGCCTGAAGCCTGTGCAGCTGAGCAGCGGCCAGTACGAG
  TGCCGGCCCCAGCTGCAACTGCCCTGGGTGCCCCGGCCCAGCAGCTTCATGTACGACTCCTACCGGCTGT
  GGTATGAGAAGCGGTGGCTGACCATCATCCTGTACGTGTTCATGTGGACCTACCTGGTGACCCTGCTGCA
  GTACTGCATCGTGAGATTCATCGGGACCCGGCTGTTCTACTTCCTCCAGAGAAACATTACCATCCGGTTC
  ACCGGCAAGCCCACCTACAACCTGCTGACATATCCCGTGAAGGGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101324 representing YP_081592
  Red=Cloning sites Green=Tags
  
  MRIQLLLVSTLVASIVATRVEDMATFRTEKQWQQDLQYRREFVKRQLAPKPKSNIVVSHTVSCVIDGGNM
  TSVWRFEGQFNPHIASEVILHDTSGLYNVPHEVQNDGQVLTVTVKRSAPADIAKVLISLKPVQLSSGQYE
  CRPQLQLPWVPRPSSFMYDSYRLWYEKRWLTIILYVFMWTYLVTLLQYCIVRFIGTRLFYFLQRNITIRF
  TGKPTYNLLTYPVKG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 675 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081592
• RefSeq ORF: 675
• Locus ID: 3077535
• MW: 26.3 kDa