US27 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101343

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein US27 [Human herpesvirus 5], codon optimized for human cell expression, YP_081611

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US27
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101343 represents NCBI reference of YP_081611 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACTAGCACCACTACCACCACCAACATCATGCTGCAGGTCTCCAACGTGACCAACCACACCCTCA
  ATAGCACCGAGATCTACCAGCTCTTCGAGTACACCCGGTTTGGCGTCTGGCTGATGTGCATCGTGGGCAC
  ATTTCTCAACATGCTGGTCATTACTACCATCCTCTACTACCGGCGGAAAAAGAAAAGCCCCAGCGACACC
  TACATTTGCAACCTCGCCGTGGCCGACCTGCTCATCGTCGTGGGACTCCCCTTTTTCCTCGAGTACGCCA
  AGCACCACCCCAAGCTGTCCCGGGAGGTCGTGTGCAGCGGGCTGAACGCCTGCTTCTACATCTGCCTCTT
  CGCCGGCGTGTGCTTTCTGATCAACCTGAGCATGGATCGGTACTGCGTGATCGTGTGGGGGGTGGAGCTG
  AACCGGGTGCGGAACAACAAGCGGGCCACATGCTGGGTGGTGATCTTTTGGATTCTGGCAGCCCTGATGG
  GCATGCCCCACTACCTCATGTACAGCCACACCAATAACGAGTGCGTCGGCGAGTTCGCAAACGAGACCAG
  CGGCTGGTTCCCCGTGTTCCTGAACACCAAGGTGAACATCTGCGGCTACCTGGCACCCATCGTGCTGATG
  GCCTACACTTACAACCGGATGGTCCGGTTCATCATCAACTATGTGGGCAAATGGCACATGCAGACTCTCC
  ACGTCCTGCTGGTCGTGGTGGTGTCCTTCGCATCCTTCTGGTTTCCCTTCAACCTCGCCCTCTTCCTGGA
  GAGCATCAGACTGCTGAGCGGCACCCAAAATGAGACCCTGCAAACCGTGATCACCTTTTGCCTCTACGTG
  GGGCAGTTCCTGGCCTACGTGCGGGCCTGCCTGAATCCCGGCATCTACATCCTGGTCGGGACCCAGATGC
  GGAAGGACATGTGGACCACACTGCGGGTGTTCGCCTGTTGCTGCGTGAAGCAGGAAATCCCCTATCAGGA
  CATCGACATTGAGCTGCAGAAGGATATTCAGCGGCGGGCTAAGCACACCAAGCGGACTCACTACGATCGG
  AAACACGCCCCCATGGAATCCGGGGAAGAGGAGTTCCTGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101343 representing YP_081611
  Red=Cloning sites Green=Tags
  
  MTTSTTTTTNIMLQVSNVTNHTLNSTEIYQLFEYTRFGVWLMCIVGTFLNMLVITTILYYRRKKKSPSDT
  YICNLAVADLLIVVGLPFFLEYAKHHPKLSREVVCSGLNACFYICLFAGVCFLINLSMDRYCVIVWGVEL
  NRVRNNKRATCWVVIFWILAALMGMPHYLMYSHTNNECVGEFANETSGWFPVFLNTKVNICGYLAPIVLM
  AYTYNRMVRFIINYVGKWHMQTLHVLLVVVVSFASFWFPFNLALFLESIRLLSGTQNETLQTVITFCLYV
  GQFLAYVRACLNPGIYILVGTQMRKDMWTTLRVFACCCVKQEIPYQDIDIELQKDIQRRAKHTKRTHYDR
  KHAPMESGEEEFLL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 1092 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081611
• RefSeq ORF: 1092
• Locus ID: 3077557
• MW: 42.2 kDa