UL73 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101252

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein N [Human herpesvirus 5], codon optimized for human cell expression, YP_081521

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL73
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101252 represents NCBI reference of YP_081521 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGTGGAACACTCTGGTTTTGGGTCTGCTTGTTCTCTCAGTGGTCGCATCCTCCAATAACACCAGCA
  CTGCTTCTACCCCAAGACCAAGCAGCTCAACTCACGCTAGCACTACAGTGAAGGCGACTACAGTCGCCAC
  AACGTCTACAACGACAGCTACGAGCACTTCTAGCACCACCTCTGCCAAGCCTGGATCTACCACGCACGAC
  CCTAATGTCATGCGGCCACATGCCCATAATGACTTTTACAATGCACACTGCACTAGTCATATGTACGAGC
  TGAGCTTGAGCAGCTTCGCTGCCTGGTGGACAATGCTGAACGCTCTGATCTTGATGGGGGCCTTTTGCAT
  CGTGCTGAGACACTGCTGTTTTCAGAATTTCACCGCTACCACAACGAAGGGGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101252 representing YP_081521
  Red=Cloning sites Green=Tags
  
  MEWNTLVLGLLVLSVVASSNNTSTASTPRPSSSTHASTTVKATTVATTSTTTATSTSSTTSAKPGSTTHD
  PNVMRPHAHNDFYNAHCTSHMYELSLSSFAAWWTMLNALILMGAFCIVLRHCCFQNFTATTTKGY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 405 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081521
• RefSeq ORF: 405
• Locus ID: 3077417
• MW: 14.5 kDa