UL148B (NC_006273) Virus Tagged ORF Clone

CAT#: VC101315

Myc-DDK-tagged ORF clone of viral ORF for protein UL148B [Human herpesvirus 5], codon optimized for human cell expression, YP_081583

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL148B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101315 represents NCBI reference of YP_081583 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTTTGGATACATTGGCCGGGCTTGCTATTTGCGTAGGACTCGTGATGGGCGTCACTGTAATCGCTA
  GCTGTGCTCTGCTTGTGTTCTACTATTGCGACGAGCGGGAAGACGGGCGACCGTCCAAGCTGCTCCAAAG
  ATCCATCCGCCGGTGGCGGCACGGCCTTTCTACTGAGAGCCTGACGGCTATCCTGCCAGACGGGTCCAGC
  ACTGAGCAGGAAATCTACCACACCAGACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101315 representing YP_081583
  Red=Cloning sites Green=Tags
  
  MALDTLAGLAICVGLVMGVTVIASCALLVFYYCDEREDGRPSKLLQRSIRRWRHGLSTESLTAILPDGSS
  TEQEIYHTRL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 240 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081583
• RefSeq ORF: 240
• Locus ID: 3077453
• MW: 8.9 kDa