UL72 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101251

Myc-DDK-tagged ORF clone of viral ORF for deoxyuridine triphosphatase [Human herpesvirus 5], codon optimized for human cell expression, YP_081520

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL72
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101251 represents NCBI reference of YP_081520 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGACCATGCTGACCGATCGCATTGACTCCCAGCTGGTGCTCTCCAGGTTGCCACGAAGTAGGTTTC
  AGCGATTTTGGGAAACGCCTACCCTGATCATGAAGGAAGAGTCAGCCCCCAGCTCTGGGTCTATTATACT
  CGCAGAAAAATCCGTGAACATGAGATATTGCGTACGATTTGCCTCAGACTCCGACTTCCAAACTACGTTC
  ACTTTGCCTCAATCTACAGAAGAGAAGTACGATAAGGAGCAACACCCAGGTGAAGACGAGGCGTCCTCTC
  CGCTCCCAAGCCCTCTGAAGGTCCCCTATAAGTGGATGCCTAGCTCTTTCATAGTGAAACAGTGCCATAC
  GCAGCTGGCGTTCTATAATAAACACATTATTTGGCTCAGTCGGGAGCGCAAAGTGCCTACCTCCCTGGGC
  GTTTCTCTCTACATCCCAGAGGGCTTTTTTGGCATTACGTTTTATAAGTGCCTGGACGCCCAATTTGTGT
  GTATGCCCGAGTTGCTGGAGAGCGGTTTGCAAGTGCCTCAGCTGGACGTGGTGAACCTGAATGATACTTT
  TCAAAGCATATTCCCCGGCACCATCGAAGGAGACATCGGCGTGTTCCCATGCTTCGTTCCAGAACCATGG
  CAGCTGATGAACCTCCCGCCACCTAACGAGCATAGGTTCTTCTCCCTGCGCACAAGGCAAACTCTGGTGA
  TTGGCCCAGGCCACACCCAGACGGTGTACTTCGATGCAGCTTATGTGCACGCTCCCGGTATATGTGCACT
  TATTGTGGGCGTCAGACAGTTCTCCCAGTCCGATCTGATTATCCGGCCAACTATCTGGCTTCCCGGAACA
  GCCGCAGGGGTCACGGTTGTCAATACCTCCCATACTACTGTCTGTATAAGCCCACATACCACCGTGGCCA
  AAGCAGTTTTTACCACACATCGGTTCACATACCTGCCCGTCGGTTCCCATCCACTCGGGCAGATGATCGT
  GCCCCCCACCCCCGATATAGGATTTACACATACACCCGAACATGCTCTGTTGCAACGGACACCATCCCCC
  GTGGACGATGACGTTGATGAGACCGAAGAGGACGAAAAATCAAGTGACGCAGAATCACCGGTTAACACCT
  CCGATGTCATCTTTGACGTCGGACCTAAGCCACCAAGGCACCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101251 representing YP_081520
  Red=Cloning sites Green=Tags
  
  MLTMLTDRIDSQLVLSRLPRSRFQRFWETPTLIMKEESAPSSGSIILAEKSVNMRYCVRFASDSDFQTTF
  TLPQSTEEKYDKEQHPGEDEASSPLPSPLKVPYKWMPSSFIVKQCHTQLAFYNKHIIWLSRERKVPTSLG
  VSLYIPEGFFGITFYKCLDAQFVCMPELLESGLQVPQLDVVNLNDTFQSIFPGTIEGDIGVFPCFVPEPW
  QLMNLPPPNEHRFFSLRTRQTLVIGPGHTQTVYFDAAYVHAPGICALIVGVRQFSQSDLIIRPTIWLPGT
  AAGVTVVNTSHTTVCISPHTTVAKAVFTTHRFTYLPVGSHPLGQMIVPPTPDIGFTHTPEHALLQRTPSP
  VDDDVDETEEDEKSSDAESPVNTSDVIFDVGPKPPRHP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 1164 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081520
• RefSeq ORF: 1164
• Locus ID: 3077454
• MW: 43.5 kDa