US13 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101330

Myc-DDK-tagged ORF clone of viral ORF for membrane protein US13 [Human herpesvirus 5], codon optimized for human cell expression, YP_081598

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101330 represents NCBI reference of YP_081598 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCCCCCCCTGCCCAGCCTGCACAGCCCCCAGTGGGCCAGCCTGCTGCAACTCCACCACGGCCTGA
  TGTGGCTGCGGCGGTTCGCAGTGCTGGTGCGGGTGTACGCCCTCGTCGTCTTTCACATCGCCATCAGCAC
  CGCTTTCTGCGGCATGATCTGGCTGGGCATTCCCGACAGCCACAACATCTGCCAACACGAGAGCAGCCCC
  CTGCTGCTCGTCTTCGCCCCCTCCCTCCTGTGGTGCCTGGTGCTGATTCAGGGCGAGCGGCACCCAGACG
  ATGTGGTGCTGACCATGGGGTACGTGGGCCTGCTCTCCGTGACCACCGTGTTTTACACTTGGTGCAGCGA
  CCTGCCCGCAATCCTGATCGACTACACCCTCGTGCTGACACTGTGGATTGCCTGCACTGGCGCCGTCATG
  GTCGGCGACAGCTTCCGGGCCAAGCGGTGGGAGCTCATCTGTAGCCGGGTGCTCACCAGCGTGTTCTTCA
  TCACCCTGTGGGTCATTGGCGATCAGACCGTGTTCCATCACCAGCGGATTCTGCTGTACGGCTACGGCGC
  CATCGTGTTCCTGATGATGACCGTCACTTTCTACGGCACCCGGTACATTCGGGACGAGCTGCCTGCCGCC
  CAAACCCTCCGGGGCTCCCTGCTGATTTACGTGGGCCTCGTCACAATGTTCAAAATCACCCTGATCGTGC
  TCAGCCCCAACCTCTGGCGGCTGCCATGGACAACTGTGTTCGCAGCCTTCCGGTCCAGCTACTGCGAGGG
  GGGCGGCGGGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101330 representing YP_081598
  Red=Cloning sites Green=Tags
  
  MDPPLPSLHSPQWASLLQLHHGLMWLRRFAVLVRVYALVVFHIAISTAFCGMIWLGIPDSHNICQHESSP
  LLLVFAPSLLWCLVLIQGERHPDDVVLTMGYVGLLSVTTVFYTWCSDLPAILIDYTLVLTLWIACTGAVM
  VGDSFRAKRWELICSRVLTSVFFITLWVIGDQTVFHHQRILLYGYGAIVFLMMTVTFYGTRYIRDELPAA
  QTLRGSLLIYVGLVTMFKITLIVLSPNLWRLPWTTVFAAFRSSYCEGGGGS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 783 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081598
• RefSeq ORF: 783
• Locus ID: 3077576
• MW: 29.5 kDa