US8 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101325

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein US8 [Human herpesvirus 5], codon optimized for human cell expression, YP_081593

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101325 represents NCBI reference of YP_081593 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGCGGTGGCTGCGGCTGCTGGTGGGACTGGGCTGTTGCTGGGTGACCCTCGCCCACGCCGGAAACC
  CCTATGAGGACGACGACTACTACTACTATCGGGAGGACGAACCACGGCAACACGGCGAGCCCAACTACGT
  CGCCCCCCCCGCCCGGCAGTTCCGGTTCCCCCCCCTGAACAATGTGAGCAGCTACCAGGCCAGCTGCGTC
  GTGAAAGACGGCGTGCTGGATGCCGTGTGGCGGGTGCAGGGCACCTTCTACCCCGAGAAGGGCATCGTGG
  CCCGGGTGGGCTGGAGCGGGCGGCGGGGACGGAAATGGGGCCGGCTGCACGCCCCCGAGTGCCTGGTGGA
  GACCACCGAGGCCGTGTTCCGGCTGCGGCAGTGGGTGCCCACAGACCTGGACCACCTGACCCTGCACCTG
  GTGCCCTGCACCAAGTGCAAGCCCATGTGGTGCCAGCCCCGGTATCACATCCGGTACTTTAGCTACGGCA
  ACAGCGTGGACAACCTGCGGCGGCTGCACTACGAGTACAGACATCTGGAGCTGGGCGTGGTGATCGCCAT
  TCAGATGGCCATGGTGCTGCTGCTCGGCTACGTGCTGGCCCGGACCGTGTATCGGGTCTCCAGCGCCTAC
  TACCTGCGGTGGCACGCCTGTGTGCCCCAGAAATGTGAGAAAAGCCTGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101325 representing YP_081593
  Red=Cloning sites Green=Tags
  
  MRRWLRLLVGLGCCWVTLAHAGNPYEDDDYYYYREDEPRQHGEPNYVAPPARQFRFPPLNNVSSYQASCV
  VKDGVLDAVWRVQGTFYPEKGIVARVGWSGRRGRKWGRLHAPECLVETTEAVFRLRQWVPTDLDHLTLHL
  VPCTKCKPMWCQPRYHIRYFSYGNSVDNLRRLHYEYRHLELGVVIAIQMAMVLLLGYVLARTVYRVSSAY
  YLRWHACVPQKCEKSLC
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 681 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081593
• RefSeq ORF: 681
• Locus ID: 3077426
• MW: 26.7 kDa