UL142 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101306

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein UL142 [Human herpesvirus 5], codon optimized for human cell expression, YP_081574

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL142
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101306 represents NCBI reference of YP_081574 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGATCGAGTGGGCCTGCTGGCTGTTCGGCTACTTCGTGAGCAGCGTCGGCTCCGAGCGGAGCCTCA
  GCTACCGGTACCACCTCGAGAGCAACTCCAGCGCCAACGTGGTGTGCAACGGCAATATCAGCGTGTTCGT
  CAACGGCACCCTCGGCGTGCGGTACAACATCACCGTGGGCATTAGCAGCAGCCTGCTGATCGGCCACCTC
  ACTATCCAGACCCTGGAGTCCTGGTTCACCCCCTGGGTGCAGAACAAGAGCTACAGCAAGCAGCCCCTGA
  GCACCACCGAGACCCTGTACAACATCGACTCCGAGAACATTCACCGGGTGTCCCAGTACTTCCACACCCG
  GTGGATCAAGAGCCTCCAGGAGAACCACACCTGCGACCTCACCAACTCCACCCCCACCTACACCTACCAG
  GCCAACGTGAACAACACCAACTACCTGACCCTGACCAGCAGCGGGTGGCAGGACCGGCTGAACTACACCG
  CCATTAACAGCACTCACTTCAACCTGACCGAGTCCAACATCACCTCCATCCACAAGTACCTGAACACCAC
  CTGCATCGAACGGCTGCGGAACTACACCCTGGAACCCGTGTACACCACCGCCGTCCCCCAGAACGTGACC
  CCCGAGCATGCCATCACAACCCTCTACACCACCCCCCCCAACGCAATCACCATCAAGGACACAACCCAGA
  GCCACACCGTGCAAACACCCAGCTTCAATGACACCCACAACGTGACCGAACACACACTGAACATCAGCTA
  CGTGCTGAGCCAGAAGACCAACAATACCACCAGCCCATGGGTCTACGCCATTCCCATGGGGGCAACCGCT
  ACCATCGGCGCCGGACTCTACATCGGCAAGCATTTCACCCCAGTGAAGTTCGTCTACGAAGTCTGGCGGG
  GGCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101306 representing YP_081574
  Red=Cloning sites Green=Tags
  
  MRIEWACWLFGYFVSSVGSERSLSYRYHLESNSSANVVCNGNISVFVNGTLGVRYNITVGISSSLLIGHL
  TIQTLESWFTPWVQNKSYSKQPLSTTETLYNIDSENIHRVSQYFHTRWIKSLQENHTCDLTNSTPTYTYQ
  ANVNNTNYLTLTSSGWQDRLNYTAINSTHFNLTESNITSIHKYLNTTCIERLRNYTLEPVYTTAVPQNVT
  PEHAITTLYTTPPNAITIKDTTQSHTVQTPSFNDTHNVTEHTLNISYVLSQKTNNTTSPWVYAIPMGATA
  TIGAGLYIGKHFTPVKFVYEVWRGQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 915 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081574
• RefSeq ORF: 915
• Locus ID: 3077552
• MW: 34.6 kDa