UL53 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101243

Myc-DDK-tagged ORF clone of viral ORF for nuclear egress lamina protein [Human herpesvirus 5], codon optimized for human cell expression, YP_081512

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL53
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101243 represents NCBI reference of YP_081512 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAGTGTTAGCGGAGTGCGCACACCCCGGGAGAGGCGATCTGCATTGAGGTCACTCCTTCGGAAGC
  GCAGACAGAGAGAACTCGCCAGCAAAGTAGCCTCTACTGTAAACGGTGCCACTTCCGCGAATAACCACGG
  TGAACCTCCAAGCCCGGCTGATGCCAGACCTCGGCTGACTCTGCACGATCTTCATGATATCTTCAGGGAA
  CATCCCGAGCTTGAATTGAAGTACTTGAACATGATGAAAATGGCTATCACTGGCAAGGAGTCCATTTGCC
  TGCCATTTAACTTTCATAGCCACCGCCAGCACACTTGCCTGGACATCAGCCCGTACGGCAACGAACAAGT
  CTCTCGAATCGCCTGCACCAGTTGCGAAGATAACCGAATCCTCCCAACTGCTTCTGACGCCATGGTTGCC
  TTCATCAACCAAACTAGTAACATAATGAAAAATCGGAACTTCTACTACGGCTTTTGCAAGTCCTCCGAAC
  TCCTGAAGTTGTCTACTAACCAGCCACCCATATTCCAGATCTATTATTTGCTCCATGCTGCAAACCATGA
  TATCGTGCCCTTCATGCATGCTGAAAATGGGCGACTCCATATGCACGTCATCTTCGAAAACTCCGATGTT
  CACATTCCATGTGATTGTATTACTCAGATGTTGACCGCTGCACGCGAAGACTACTCCGTTACACTCAATA
  TAGTGAGGGACCATGTGGTGATCTCCGTGTTGTGCCACGCCGTTAGCGCTTCTTCAGTGAAAATCGACGT
  GACCATACTTCAGAGGAAAATCGATGAAATGGATATACCAAATGACGTCTCAGAGAGCTTCGAGAGGTAT
  AAAGAATTGATTCAGGAACTTTGCCAGTCTAGCGGCAACAATCTTTACGAGGAGGCCACCTCATCCTACG
  CTATCCGCTCTCCACTGACCGCTAGCCCTCTGCATGTTGTGAGTACAAATGGGTGCGGTCCCAGTTCTTC
  TTCTCAGTCCACACCTCCTCACCTCCACCCACCAAGTCAGGCAACCCAGCCTCATCATTATAGCCACCAT
  CAATCCCAGTCACAGCAGCACCATCATAGGCCCCAGTCTCCCCCTCCTCCCCTGTTCCTGAACTCCATTC
  GAGCTCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101243 representing YP_081512
  Red=Cloning sites Green=Tags
  
  MSSVSGVRTPRERRSALRSLLRKRRQRELASKVASTVNGATSANNHGEPPSPADARPRLTLHDLHDIFRE
  HPELELKYLNMMKMAITGKESICLPFNFHSHRQHTCLDISPYGNEQVSRIACTSCEDNRILPTASDAMVA
  FINQTSNIMKNRNFYYGFCKSSELLKLSTNQPPIFQIYYLLHAANHDIVPFMHAENGRLHMHVIFENSDV
  HIPCDCITQMLTAAREDYSVTLNIVRDHVVISVLCHAVSASSVKIDVTILQRKIDEMDIPNDVSESFERY
  KELIQELCQSSGNNLYEEATSSYAIRSPLTASPLHVVSTNGCGPSSSSQSTPPHLHPPSQATQPHHYSHH
  QSQSQQHHHRPQSPPPPLFLNSIRAP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 1128 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081512
• RefSeq ORF: 1128
• Locus ID: 3077575
• MW: 42.3 kDa