UL47 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101236

Myc-DDK-tagged ORF clone of viral ORF for tegument protein UL37 [Human herpesvirus 5], codon optimized for human cell expression, YP_081505

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL47
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101236 represents NCBI reference of YP_081505 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCTCGCAGGACGGTCGATTTTAAGAAGCTGATCGAGCAATTGCGCGCGCGCGCTACCGACAAGG
  CCGAGGCTCTGAATACCGTTAGCCAACTCGAGATCGGGGCAGTGGATGCCCAGGACGTGACGGCTAGTGC
  TGTCCGGGCTTTCGTTGGTGCGCTTCCAAGCAGCGGTTACCACTTCGGGTTCGTCAGACAGAACGTGGTT
  TTTTACCTGCTTTCACACGCCACGGTGCAGACCGCAAGGGATCCCTTGTATGCCGCAGAACAGCTGCACG
  AACAATTGGATAGGTTTTTGCGGCACCAGCATGACGGCGGGGGAGATGAAGATAGGCTGCCCTTTTATCA
  TAACGGGGCGACACTTACTGCTTTCCAAAAACTTCTGCAGACCTTGAGGGAAATTCAGACCGTGATTGCC
  GAGCAGTCAGGAGGAACAGCTGCGGCCGCCGACCTGATTGCTTCAAACAATGCTTCTACAGAACGCCGGG
  GGAAGAAGGGTGGCTCTTCCAGCGGCGGTCAGCAGCCCCTGGTCCGGAGGGTGATTACTCAACTGGAGAC
  TGCCGCTACCGAGGCTAGGCCATACGTTAATTGTCGCGCGGTAGCTGAGCTTCTCGACCTCACTTACCAG
  CGGTTGATATATTGGGCGTGCACACTTATGCCATACGTGCTCTTTAGGCGGGATACTGACACCGAACTGG
  ACACTGTGCTGCTGATGCACTTTTTCTACACTCACTATCGCTCAGTCAACGGTGACCTGGCAGTGGAGTT
  TCAGAACTACGTAAAAAATTCCGTGAGACACATGAGCTCATTTGTTTCTAGCGACATCGACGGGGATCAG
  AAGCCGGGAGCAGAGCACATGCGGGACGTCTCCTATAAACTCTTTGTGGGCAATCTGCAGGCTCGCGACG
  CCAGCGGTCTGATGTTCCCGATCATCAGCACGAGGATCAGCACGGTTAACCTGTATCTCTCCCCTGAACG
  GATGTTCTTCCACCCCGGCCTGATTTCTAGGCTCCTGTCCGAAGAAGTGAGCCCTCGCGCGAACTTGGAC
  GCTTACGCCAGGGTCTGCGACCGCGTGCTCGAGGATCATCTGCATACCCCACGGCGGGTTCAGAGGCTTT
  TGGACTTGACACAGATGGTGATGCGACTGGTCGAGCTCGGTTTCAATCACGACACTTGCGCTGCATATGC
  TCAGATGGCACTGATTCAACCTGCTTCTCAAAAAAGTAGTCTGTTTGTGAGTGAAATCCGAGAAAAGCTT
  ATTCAGATCATTTATAATTTTTACACCTTCTTTATGTGTCTTTATGTGTATTCCCCTACCTTTCTGTTCG
  ATCACCGGAGACGACTCATTCTCGAACAGCATCGCTCTACCCTGATCGGCAGCAAAGAGGAGCTTCAGCA
  CGTCTGGAGTAATGTTACTTTGAACGTGAACACCCACTTCGCGGTGCAGTATACAGAAGAAGACTTTGAA
  GCTCACACCAAAGGAGCTACCGAGGCAGAAAGGGAGTACCTTTACCGAGACCTCCACTCAAAATGGGGCG
  TGCATCTTTTCACTCTTCGACCTTCTCGCGGGGCCGCTGGGGCAGCAAGTCCACTGCCTCCACTTGACGG
  CGTCACGAGGTCAGACATTCTGCGGGAATGTGCTCTGGTCAACCTGAACGAGGGCCGAGTCAACTATGCC
  TCCTTGCTGGCATTCTCACACCACCCAGAATTTCCTTCCATTTTCGCTCAGCTCGTGGTGGTCACCGAGT
  TCAGTGAAATTTTTGGAATACCGCAGGGACTTTTCCAGGCAGTCGGTAGCCCGAGACTGTTTGCTCTCAT
  TCAGCTTTGCCGGGTCCTCTTGCCAGAGCAGGTGACTCTCTACCAGAATCTGGTGAGTATTTATAACTTG
  ACCACGTTCGTGAAACATATCGACGCTGCCGTTTTCAAGACTGTCAGGGACTGTGTGTTCGACATCGCAA
  CTACCCTCGAACACCTCTCAGGCGTCCCCGTTACCCCGAACGTCGACCTGTTGGCCGAGCTGATGGCCCG
  GTCTGTGGCGCACAACCTTTACACCACAGTCAATCCCCTTATCGAAGATGTGATGCGATCTTCCGCGGGG
  TCTCTGAGGAACTACCTGCGCCACACTCGCCTCTGCTTTGGTCTGGCTCGCGGGAGGGCCAGGCTTAGCG
  AGGACGGCGTCACCGTGTACGTGGAGGTGCAGGGCCAGTACGGGCTGCGCGTACCCACCACGCGCTTTGT
  TGAGCAGCTGAGAGAGCTGGTGCGGCGGGACAGGTTGCTGGCTGAGAACCTTAGGGGACTCAACGAACGG
  CTGCTCTCCGTAAGGGTCAGGGTGCGCCAAATCTCAAGTGATACTGAGGAGGTTTCAAGGCACGCAAAGG
  GTCACCGGACTGTCGCCCAGATGAGCAAAGCGCTTAAAAAGACCGCTTCTAAAATCAAAATGTTGGAGAC
  TCGGGTTACTCTGGCCCTCGAACAAGCCCAGCGGTCAAACGGAGCGGTCGTGACCGCGGTACAGCGCGCA
  CTGGCAGTGTTCGACGTGCTCAGCAGAGAAAACCTGGAGAGGCGCGGCGCACAGCTTTGTCTTACAGAGG
  CAACATCTCTCCTCCACAGGCATCGCGCACTCGCCCCTATGACCTGGCCAGCTGGTACAGGCGTAGCCGC
  AGCTGCCGAGGCAGACGGAGCCTTGCGAGAGTTCCTGGAGGCACCTTGGGAAAGCGCGCCACAGCCCCCC
  CGCCTGAGAATGACGCCAGACACCGACCATGAGGAATCCACCGCCGGGGCAACCTCCGTCCCCGAAGTAT
  TGGGCGCCCGCTATGAACCAGCCCATTTGGCAGCTTCCGACCTTCTCAATTGGTATATTGTACCCGTCTC
  ACAGGCCCAACAAGATATTTTGTCTAGCATAGATCCTCCCGCTGGTAGCACATCCGTTTCCCTCCCACCT
  GCCTCACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101236 representing YP_081505
  Red=Cloning sites Green=Tags
  
  MMARRTVDFKKLIEQLRARATDKAEALNTVSQLEIGAVDAQDVTASAVRAFVGALPSSGYHFGFVRQNVV
  FYLLSHATVQTARDPLYAAEQLHEQLDRFLRHQHDGGGDEDRLPFYHNGATLTAFQKLLQTLREIQTVIA
  EQSGGTAAAADLIASNNASTERRGKKGGSSSGGQQPLVRRVITQLETAATEARPYVNCRAVAELLDLTYQ
  RLIYWACTLMPYVLFRRDTDTELDTVLLMHFFYTHYRSVNGDLAVEFQNYVKNSVRHMSSFVSSDIDGDQ
  KPGAEHMRDVSYKLFVGNLQARDASGLMFPIISTRISTVNLYLSPERMFFHPGLISRLLSEEVSPRANLD
  AYARVCDRVLEDHLHTPRRVQRLLDLTQMVMRLVELGFNHDTCAAYAQMALIQPASQKSSLFVSEIREKL
  IQIIYNFYTFFMCLYVYSPTFLFDHRRRLILEQHRSTLIGSKEELQHVWSNVTLNVNTHFAVQYTEEDFE
  AHTKGATEAEREYLYRDLHSKWGVHLFTLRPSRGAAGAASPLPPLDGVTRSDILRECALVNLNEGRVNYA
  SLLAFSHHPEFPSIFAQLVVVTEFSEIFGIPQGLFQAVGSPRLFALIQLCRVLLPEQVTLYQNLVSIYNL
  TTFVKHIDAAVFKTVRDCVFDIATTLEHLSGVPVTPNVDLLAELMARSVAHNLYTTVNPLIEDVMRSSAG
  SLRNYLRHTRLCFGLARGRARLSEDGVTVYVEVQGQYGLRVPTTRFVEQLRELVRRDRLLAENLRGLNER
  LLSVRVRVRQISSDTEEVSRHAKGHRTVAQMSKALKKTASKIKMLETRVTLALEQAQRSNGAVVTAVQRA
  LAVFDVLSRENLERRGAQLCLTEATSLLHRHRALAPMTWPAGTGVAAAAEADGALREFLEAPWESAPQPP
  RLRMTPDTDHEESTAGATSVPEVLGARYEPAHLAASDLLNWYIVPVSQAQQDILSSIDPPAGSTSVSLPP
  ASP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 2949 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081505
• RefSeq ORF: 2949
• Locus ID: 3077521
• MW: 110.0 kDa