UL148A (NC_006273) Virus Tagged ORF Clone

CAT#: VC101314

Myc-DDK-tagged ORF clone of viral ORF for protein UL148A [Human herpesvirus 5], codon optimized for human cell expression, YP_081582

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL148A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101314 represents NCBI reference of YP_081582 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAAGCGACAGTAATTTTGACCCATGGATCCCAGTCTGTGTTGTGGTAGTCATGACATCCGTGGTTC
  TGTTCGCCGGTCTGCACGTATATCTGTGGTACGTCCGCCGACAGCTGGTAGCCTTCTGTCTCGAGAAAGT
  ATGCGTCAGATGCTGTGGAAAGGATGAAACCACACCTTTGGTAGAAGACGCGGAGCCTCCTGCGGAGCTC
  GAAATGGTAGAAGTAAGCGATGAGTGCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101314 representing YP_081582
  Red=Cloning sites Green=Tags
  
  MSSDSNFDPWIPVCVVVVMTSVVLFAGLHVYLWYVRRQLVAFCLEKVCVRCCGKDETTPLVEDAEPPAEL
  EMVEVSDECY
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 240 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081582
• RefSeq ORF: 240
• Locus ID: 3077461
• MW: 9.1 kDa