UL29 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101219

Myc-DDK-tagged ORF clone of viral ORF for protein UL29 [Human herpesvirus 5], codon optimized for human cell expression, YP_081487

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL29
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101219 represents NCBI reference of YP_081487 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGGCAGAAGAAAAGGATGTAGTGCTGCAACCGCATCTTCATCCTCCTCAAGTCCACCATCTCGGC
  TTCCTCTGCCAGGGCACGCCCGGCGACCCAGACGGAAGAGATGCTTGGTCCCCGAGGTCTTCTGTACCCG
  CGACCTTGCCGATCTGTGTGTGCGCCGGGACTATGAAGGTCTGAGGAGATACCTTAGACGGTTTGAGGGC
  AGCTGCGTAAGCCTGGGCTGGCCCTCCCAGTGCATCTACGTTGTGGGCGGCGAACATAGTCCGCACAGCC
  TGACCGAAATCGACCTGGAGCACTGTCAGAACGATTTCTTTGGTGAGTTCAGAGCGCTGCATCTGATCGG
  CACAGTCTCCCATGCAACATGCAGGTACCAAGTGTTTGTGGATGCTTACGGCGCCGTGTTTGCATACGAT
  GCTCAGGAGGATTGCCTCTACGAGCTGGCCTCCGATCTTGCAGGCTTTTTCGCAAAGGGAATGATCCGGT
  GCGATCCCGTCCATGAGTCAATTTGCGCCAGGCTCCAGCCCAACGTGCCCCTGGTGCACCCTGATCACCG
  AGCGGAGTTGTGCCGACGAAGTAGGGCCTCCGCACGCGGACGCTACCTGAGAAGCCTGCTCGCTTTCCGA
  GAGTTGCTGGCCTGCGAAGACACAGCCGCCCGATGTGCCTATGTGGAAGCACATAGAGAAGCCCAGCTCA
  CTCTCATTTGGCCTGAAAAACACAGCCTCGTGCTGAGGACTGCCAGGGACCTGGGCCTCTCAGCATCAAT
  GCTCCGCAGATTTCAGCGGTCACTGTATACTAGAGAGCCAGTCATGCCCCTCGGGGAGATTGAGGGCGCG
  GAAGATAAGACGTTCTTTCACAGGGTCCGCATCTTGTGCGGCGATACTGGAACCGTTTACGCTGCTCTCG
  TCGGACAGGACAAACTCGTCAGATTGTCCAGGGATCTCCGCGGCTTCGTGAGAGTAGGTCTCGCTCTGCT
  GATCGACGATTTTAGGTACGAATCTATTGGCCCAGTTGACAGGTCTTCATTGTATGAGGCAAATCCCGAG
  CTCAGGCTGCCCTTCAAGAAGAGGCGCCTGGTGGTGGGCTACTTTGACTCCCTTAGCTCACTGTACCTCC
  GGGGACAACCCAAATTCTCATCCATATGGCGAGGATTGAGGGACGCATGGACTCACAAACGACCAAAGCC
  CCGCGAAAGAGCTAGTGGGGTGCATCTCCAGAGGTATGTCAGAGCTACTGCCGGAAGATGGCTTCCCCTG
  TGTTGGCCCCCCCTGCACGGCATCATGCTGGGAGACACGCAATATTTCGGGGTAGTGCGCGACCACAAAA
  CATACCGACGCTTCTCATGCCTGCGCCAGGCGGGCAGACTGTACTTTATCGGGCTTGTCAGCGTTTATGA
  ATGTGTTCCCGATGCTAACACCGCTCCTGAAATCTGGGTTTCTGGCCACGGTCACGCCTTTGCATACCTG
  CCCGGCGAAGATAAGGTGTACGTTTTGGGGCTTTCTTTTGGAGAGTTTTTCGAAAATGGGCTGTTTGCCG
  TGTACTCCTTCTTCGAGCGCGATTACGTGGATGAAATCGTTGAGGGCGCATGGTTTAAGCACACTTTTGC
  AGGTATGTACGAACTCAGTCAAATACTTCATGATCGGGCCAACCTTCTCCGAGTATGTCAGTTGCACGCC
  GGCAGCAAGATACGGCTGGGAGGTAGCCCAGCCTGCACCTTCACCTTCGGGTCATGGAATGTGGCCGAGG
  CAGATGAGGCGAATAACTTTGTCATCGGAGTGCTGGAACAGGCTCACTTCGTCGTTATCGGATGGATGGA
  ACCCGTGAATAAGGCAGTGTTCATGGATGCCCATGGTGGCATCCACGTGCTGCTGTACGGAACAATGCTG
  GTGAAACTTGCCGAAACCCTGAGGGGCTTCATTCGGCAGGGAAGCTTTTGGTTTCGGTGCCCGCGACGGT
  TCTGCTTCTCCCCACTCGATTCTAGTGCCACAGTTGCAGCTAAACCCGTTAGTAGTCACACATCACCCGC
  CTACGATGTCTCTGAGTACGTGTTTTCTGGGCGGAGTGTACTGGACTCCGTGTCTGGAACCGGGGCTAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101219 representing YP_081487
  Red=Cloning sites Green=Tags
  
  MSGRRKGCSAATASSSSSSPPSRLPLPGHARRPRRKRCLVPEVFCTRDLADLCVRRDYEGLRRYLRRFEG
  SCVSLGWPSQCIYVVGGEHSPHSLTEIDLEHCQNDFFGEFRALHLIGTVSHATCRYQVFVDAYGAVFAYD
  AQEDCLYELASDLAGFFAKGMIRCDPVHESICARLQPNVPLVHPDHRAELCRRSRASARGRYLRSLLAFR
  ELLACEDTAARCAYVEAHREAQLTLIWPEKHSLVLRTARDLGLSASMLRRFQRSLYTREPVMPLGEIEGA
  EDKTFFHRVRILCGDTGTVYAALVGQDKLVRLSRDLRGFVRVGLALLIDDFRYESIGPVDRSSLYEANPE
  LRLPFKKRRLVVGYFDSLSSLYLRGQPKFSSIWRGLRDAWTHKRPKPRERASGVHLQRYVRATAGRWLPL
  CWPPLHGIMLGDTQYFGVVRDHKTYRRFSCLRQAGRLYFIGLVSVYECVPDANTAPEIWVSGHGHAFAYL
  PGEDKVYVLGLSFGEFFENGLFAVYSFFERDYVDEIVEGAWFKHTFAGMYELSQILHDRANLLRVCQLHA
  GSKIRLGGSPACTFTFGSWNVAEADEANNFVIGVLEQAHFVVIGWMEPVNKAVFMDAHGGIHVLLYGTML
  VKLAETLRGFIRQGSFWFRCPRRFCFSPLDSSATVAAKPVSSHTSPAYDVSEYVFSGRSVLDSVSGTGAS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 2100 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081487
• RefSeq ORF: 2100
• Locus ID: 3077519
• MW: 79.0 kDa