US30 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101346

Myc-DDK-tagged ORF clone of viral ORF for membrane protein US30 [Human herpesvirus 5], codon optimized for human cell expression, YP_081614

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US30
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101346 represents NCBI reference of YP_081614 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCAACCCCCGGAGCCCCCTCGACCGGAGACTCAGATTCAGAAACGTCCCCTTTCTGCCCCCAAGAA
  TCGCCGCCAGCTCCACAACCACCGCCAGAAGCCTCAAGGCCAAGACCATGGAAATGCGGTTCACCATCGC
  CTGGATGTGGTTCCCCAGCGTGCTGCTGATCCTGGGCCTGCTCACCCCCCCCTCCAACGGGTGTACCGTG
  GACGTGGGACGGAACGTGTCCATTCGGGAGCAGTGCAGACTCCGGAATGGCGCCACCTTCAGCAAGGGCG
  ACATTGAGGGGAACTTCAGCGGGCCAGTGGTGGTGGAGCTGGACTACGAAGACATCGACATCACCGGCGA
  GCGGCAACGGCTGCGGTTCCACCTGAGCGGCCTGGGGTGCCCCACCAAAGAGAACATCCGGAAGGACAAC
  GAGAGCGACGTGAACGGCGGCATCCGGTGGGCCCTGTACATCCAGACTGGCGACGCCAAGTACGGCATCC
  GGAACCAGCACCTGTCCATCCGGCTGATGTACCCCGGCGAGAAGAACACCCAGCAGCTGCTGGGCTCCGA
  CTTCTCCTGTGAACGGCACCGGCGGCCTAGCACCCCCCTGGGCAAGAATGCCGAGGTGCCCCCCGCCACT
  CGGACAAGCTCCACCTACGGCGTGCTGAGCGCCTTCGTCGTGTGGATCGGGAGCGGCCTGAACATCATCT
  GGTGGACAGGCATCGTGCTGCTGGCCGCCGACGCCCTGGGCCTGGGCGAGAGATGGCTCCGGCTGGCCCT
  CAGCCACAGAGACAAGCACCACGCCAGCCGGACCGCCGCCCTCCAGTGCCAGCGGGACATGCTCCTGCGG
  CAGCGGCGGCGGGCTCGGCGGCTGCACGCCGTGAGCGAGGGCAAGCTGCAGGAGGAGAAGAAGCGGCAGA
  GCGCCCTGGTGTGGAACGTGGAGGCCCGGCCCTTCCCCAGCACCCACCAGCTCATCGTGCTGCCACCCCC
  CGTGGCCTCCGCCCCTCCCGCCGTCCCCAGCCAGCCCCCCGAATATAGCTCCGTCTTCCCCCCCGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101346 representing YP_081614
  Red=Cloning sites Green=Tags
  
  MGNPRSPLDRRLRFRNVPFLPPRIAASSTTTARSLKAKTMEMRFTIAWMWFPSVLLILGLLTPPSNGCTV
  DVGRNVSIREQCRLRNGATFSKGDIEGNFSGPVVVELDYEDIDITGERQRLRFHLSGLGCPTKENIRKDN
  ESDVNGGIRWALYIQTGDAKYGIRNQHLSIRLMYPGEKNTQQLLGSDFSCERHRRPSTPLGKNAEVPPAT
  RTSSTYGVLSAFVVWIGSGLNIIWWTGIVLLAADALGLGERWLRLALSHRDKHHASRTAALQCQRDMLLR
  QRRRARRLHAVSEGKLQEEKKRQSALVWNVEARPFPSTHQLIVLPPPVASAPPAVPSQPPEYSSVFPPV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 1047 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081614
• RefSeq ORF: 1047
• Locus ID: 3077526
• MW: 39.1 kDa