UL20 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101211

Myc-DDK-tagged ORF clone of viral ORF for membrane protein UL20 [Human herpesvirus 5], codon optimized for human cell expression, YP_081479

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101211 represents NCBI reference of YP_081479 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCGGTATAAGAGCGATGCTTGTAATGCTCGATTATTATTGGATACAGCTTATCACGAATAACGGCA
  CGCGCTCCAATAATACAGACACAATTTTCGTCAGCCTGTTGACCGGCCCAAATGGTGTGACACGGACAGC
  CATTGGCGGTCTGTACTCTAACTACACAAATTTGACCGGAGCCTTTGGATTTACATCTACAAACATGAGC
  GCAACCAACTCTTCCGCTGAAGATAACTGGTCTGTGACAAACCTGACAGAGTCTTGTATTAATAGAGGCG
  AAAGCTACGTTACCACTATCTGGTTGCTCGATTGTACAAAAAACGATACCTATTGGTACTACGGCAATGC
  TTATAACCATACCTGCGAAGGCACGATCAGCGGCTATCTGCTTGGCATGTGTAAGTTGTGGAAGAGTTGG
  GTGAATAACATCACCTCATACAACACTGTAAGGGTCGAGTCCCTGGGCAACGAAACAAGGTGCATGCTGC
  TTCCACGACAGTACACACTGAATGCTACCGTCGAATGGTATAACAAGTCCGAAGGCGATGTCCCAGAAGA
  GTTTATGGACTACGTCATCCTGACTCCCTTGGCAGTACTCACCTGTGGTCTCCAAGAGGCCTATATCCTG
  GACAAGGGACGACGCTACATGTACTTGTTCAGCGTGTCTTGCGTGGGGATCACCGGGACTGTGTCTATTA
  TTCTGGTTAGCCTCTCCCTGCTGATTCTGATTTGCTACTATAGATGCGGACGATTGTTGATTTGCCCGAG
  GGGGTTTGAACTCCTCCCCGAGTTCACAGAAGAGGAGGAGGAGAAAGAAAAACTTCTCACCCACAACGAT
  ATTGAAGTGCAGGTGCCCATACGCACTAGGAGACTGCTGGTGCCTTGGATTAGAGAGAGCAAGATGTGGG
  TCCTGCCCCCACCACTCCCCCCGCGGCCCCCTCACCTCATCGAGTTTCCACCCAGTCCACCACCCAGCCC
  TGGACCTATGCACATGGTGGTGTGCATGCCAGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101211 representing YP_081479
  Red=Cloning sites Green=Tags
  
  MLGIRAMLVMLDYYWIQLITNNGTRSNNTDTIFVSLLTGPNGVTRTAIGGLYSNYTNLTGAFGFTSTNMS
  ATNSSAEDNWSVTNLTESCINRGESYVTTIWLLDCTKNDTYWYYGNAYNHTCEGTISGYLLGMCKLWKSW
  VNNITSYNTVRVESLGNETRCMLLPRQYTLNATVEWYNKSEGDVPEEFMDYVILTPLAVLTCGLQEAYIL
  DKGRRYMYLFSVSCVGITGTVSIILVSLSLLILICYYRCGRLLICPRGFELLPEFTEEEEEKEKLLTHND
  IEVQVPIRTRRLLVPWIRESKMWVLPPPLPPRPPHLIEFPPSPPPSPGPMHMVVCMPA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 1014 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081479
• RefSeq ORF: 1014
• Locus ID: 3077434
• MW: 38.3 kDa