UL75 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101255

Myc-DDK-tagged ORF clone of viral ORF for envelope glycoprotein H [Human herpesvirus 5], codon optimized for human cell expression, YP_081523

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL75
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101255 represents NCBI reference of YP_081523 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACCAGGGTTGCCAAGCTATCTGATCATTCTGGCAGTGTGTCTGTTTAGCCACCTCCTGTCTAGTC
  GGTACGGGGCCGAGGCAGTCTCAGAGCCATTGGATAAAGCGTTCCATCTCTTGTTGAACACGTACGGAAG
  ACCTATCAGATTCCTTAGAGAAAATACAACCCAATGCACTTACAATTCTTCTCTGCGCAATTCTACCGTG
  GTGAGAGAAAACGCCATTAGTTTCAATTTTTTCCAATCATACAACCAGTACTACGTCTTTCACATGCCCC
  GATGCCTGTTCGCCGGACCCCTCGCCGAGCAGTTTCTCAACCAGGTAGATCTCACCGAAACGCTGGAGAG
  GTATCAGCAGAGGCTGAATACATACGCGCTGGTTTCCAAAGATCTGGCCAGCTATCGGAGCTTCTCTCAA
  CAACTGAAGGCACAGGATAGTCTTGGCGAGCAACCAACCACCGTCCCACCACCCATAGACCTGAGCATTC
  CACATGTGTGGATGCCACCTCAGACCACACCGCACGGATGGACAGAGTCTCACACTACAAGCGGACTGCA
  CAGGCCTCACTTCAACCAGACTTGCATCTTGTTCGATGGACACGACCTGTTGTTTAGTACAGTGACTCCT
  TGTCTGCATCAGGGCTTTTATCTGATTGATGAATTGCGGTATGTGAAAATCACTCTGACCGAGGACTTTT
  TCGTGGTCACCGTCTCCATTGACGACGATACCCCAATGCTGTTGATCTTTGGCCACCTGCCCAGAGTCCT
  GTTTAAAGCCCCTTACCAGAGAGATAACTTTATTTTGCGCCAGACGGAAAAGCATGAACTCCTCGTGCTC
  GTGAAAAAAGATCAACTGAACCGGCATTCCTACCTGAAGGACCCGGATTTTCTGGACGCCGCGCTGGACT
  TCAACTATCTCGACCTCTCCGCCCTGCTCCGCAACAGTTTCCACCGCTACGCAGTAGACGTCCTTAAAAG
  CGGTCGGTGCCAGATGCTCGATAGGCGCACGGTCGAGATGGCCTTCGCCTATGCCCTGGCCCTGTTCGCT
  GCCGCCCGACAGGAGGAAGCAGGTGCTCAGGTATCCGTCCCACGCGCCCTGGACAGACAGGCTGCCCTTC
  TGCAGATTCAGGAATTCATGATCACCTGTCTGTCACAGACGCCCCCCAGGACCACACTCTTGCTGTACCC
  CACCGCGGTGGACTTGGCAAAAAGAGCTCTGTGGACACCCAATCAAATTACTGATATAACGAGTTTGGTG
  AGGCTCGTTTATATCTTGTCTAAGCAGAATCAGCAACATCTTATCCCACAATGGGCGTTGCGCCAAATAG
  CTGACTTCGCCCTGAAGCTCCATAAAACCCACTTGGCGTCCTTCCTCTCAGCGTTTGCCCGGCAGGAGCT
  CTATTTGATGGGATCTCTTGTTCACTCCATGCTTGTCCACACAACTGAAAGGCGCGAGATTTTCATCGTG
  GAGACGGGGCTGTGTTCACTCGCCGAACTGAGCCACTTTACGCAGTTGCTGGCCCACCCCCATCACGAGT
  ACCTTAGCGATCTGTACACTCCTTGCAGCTCTTCTGGAAGGAGAGACCATTCCCTCGAGAGGCTGACCCG
  GCTCTTTCCAGACGCCACAGTTCCTGCCACAGTACCTGCTGCCTTGAGTATTCTGTCTACTATGCAGCCT
  TCCACTCTGGAGACATTTCCCGACCTGTTCTGCCTGCCTCTGGGCGAGTCTTTCAGCGCTCTGACAGTGT
  CTGAGCATGTGAGCTACATAGTTACCAACCAGTACCTTATTAAGGGAATCAGCTACCCAGTTAGCACAAC
  AGTGGTGGGTCAGAGCCTGATTATTACTCAGACAGATAGTCAGACGAAGTGTGAACTCACTCGGAATATG
  CATACCACCCACTCTATCACGGTCGCACTGAACATATCACTGGAGAACTGCGCCTTCTGCCAGAGCGCGC
  TCCTGGAGTATGACGATACTCAGGGCGTCATTAACATTATGTACATGCACGATTCCGACGACGTCCTGTT
  TGCCCTCGACCCTTATAATGAAGTGGTCGTCTCATCCCCCCGAACCCACTATTTGATGCTCCTCAAGAAT
  GGCACCGTACTGGAGGTCACTGACGTGGTTGTGGACGCTACGGACAGTCGGCTGCTGATGATGAGCGTGT
  ACGCACTTTCCGCCATTATCGGAATCTATCTGCTTTATCGGATGCTCAAGACGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101255 representing YP_081523
  Red=Cloning sites Green=Tags
  
  MRPGLPSYLIILAVCLFSHLLSSRYGAEAVSEPLDKAFHLLLNTYGRPIRFLRENTTQCTYNSSLRNSTV
  VRENAISFNFFQSYNQYYVFHMPRCLFAGPLAEQFLNQVDLTETLERYQQRLNTYALVSKDLASYRSFSQ
  QLKAQDSLGEQPTTVPPPIDLSIPHVWMPPQTTPHGWTESHTTSGLHRPHFNQTCILFDGHDLLFSTVTP
  CLHQGFYLIDELRYVKITLTEDFFVVTVSIDDDTPMLLIFGHLPRVLFKAPYQRDNFILRQTEKHELLVL
  VKKDQLNRHSYLKDPDFLDAALDFNYLDLSALLRNSFHRYAVDVLKSGRCQMLDRRTVEMAFAYALALFA
  AARQEEAGAQVSVPRALDRQAALLQIQEFMITCLSQTPPRTTLLLYPTAVDLAKRALWTPNQITDITSLV
  RLVYILSKQNQQHLIPQWALRQIADFALKLHKTHLASFLSAFARQELYLMGSLVHSMLVHTTERREIFIV
  ETGLCSLAELSHFTQLLAHPHHEYLSDLYTPCSSSGRRDHSLERLTRLFPDATVPATVPAALSILSTMQP
  STLETFPDLFCLPLGESFSALTVSEHVSYIVTNQYLIKGISYPVSTTVVGQSLIITQTDSQTKCELTRNM
  HTTHSITVALNISLENCAFCQSALLEYDDTQGVINIMYMHDSDDVLFALDPYNEVVVSSPRTHYLMLLKN
  GTVLEVTDVVVDATDSRLLMMSVYALSAIIGIYLLYRMLKTC
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 2226 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081523
• RefSeq ORF: 2226
• Locus ID: 3077450
• MW: 84.3 kDa