RL5A (NC_006273) Virus Tagged ORF Clone

CAT#: VC101188

Myc-DDK-tagged ORF clone of viral ORF for protein RL5A [Human herpesvirus 5], codon optimized for human cell expression, YP_081456

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RL5A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101188 represents NCBI reference of YP_081456 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATATGAGGAAGCTCAATACAAGTCAGGGCCGAAATCTGACGACGGTGGACGACAGACGGCGCTTCA
  GCATCGGATGGGAAACCTGGGATGATGGAGGTGAATCCCTGTACGACGTTACGAATAATGGAACCACAGT
  GATAAATACCACCGCTTGTGTGTCCTCCTGTAGCCACACCTCCCTTGTTCTTTGCAACATGACCCAGCAG
  ACCGACAGCTTGTATGGAGTAGGGCACCGGCTCAACGACGAGGAGGACGGTGAGTTGTGGAGGGTGAGTG
  TGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101188 representing YP_081456
  Red=Cloning sites Green=Tags
  
  MDMRKLNTSQGRNLTTVDDRRRFSIGWETWDDGGESLYDVTNNGTTVINTTACVSSCSHTSLVLCNMTQQ
  TDSLYGVGHRLNDEEDGELWRVSVS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 285 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081456
• RefSeq ORF: 285
• Locus ID: 3077469
• MW: 10.6 kDa