US3 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101322

Myc-DDK-tagged ORF clone of viral ORF for membrane glycoprotein US3 [Human herpesvirus 5], codon optimized for human cell expression, YP_081590

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101322 represents NCBI reference of YP_081590 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCCCGTGCTGGTGCTGGCCATTCTGGCCGTGCTGTTCCTGAGACTGGCAGACAGCGTGCCCCGGC
  CCCTGAACGTGGTGGTGAGCGAGATCAAGAGCGCCCACTTCCGGGTCGAGGAGAACCAGTGCTGGTTCCA
  CATGGGCATGCTGTACTTCAAAGGCCGGATGAGCGGCAACTTCACCAAAAAGCACTTCGTCAACGTGGGC
  ATCGTCTCCCAGAGCTACATGGACCGGCTGCAGGTCAGCGGCGAGCAATACCACCACGACGAGCGGGGCG
  CCTATTTCGAATGGAACATCGGCGGCTACCCCGTCAGCCACACCGTGGACATGGTGGACATCACCCTGAG
  CACCCGGTGGGGCGACCCCAAGAAGTACGCCGCCTGCGTGCCCCAGGTGCGGATGGACTACAGCAGCCAG
  ACCATCAATTGGTACCTGCAGCGGTCCATGCGGGACGACAACTGGGGCCTCCTGTTCCGGACCCTGCTGG
  TGTACCTCTTCAGCCTGGTGGTGCTCGTCCTGCTGACCGTCGGCGTGAGCGCCAGACTGCGGTTCATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101322 representing YP_081590
  Red=Cloning sites Green=Tags
  
  MKPVLVLAILAVLFLRLADSVPRPLNVVVSEIKSAHFRVEENQCWFHMGMLYFKGRMSGNFTKKHFVNVG
  IVSQSYMDRLQVSGEQYHHDERGAYFEWNIGGYPVSHTVDMVDITLSTRWGDPKKYAACVPQVRMDYSSQ
  TINWYLQRSMRDDNWGLLFRTLLVYLFSLVVLVLLTVGVSARLRFI
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 558 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081590
• RefSeq ORF: 558
• Locus ID: 3077532
• MW: 21.6 kDa