UL99 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101278

Myc-DDK-tagged ORF clone of viral ORF for myristylated tegument protein [Human herpesvirus 5], codon optimized for human cell expression, YP_081546

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UL99
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101278 represents NCBI reference of YP_081546 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGCCGAGCTGTGCAAGCGGATCTGCTGCGAGTTCGGCACCACCAGCGGCGAGCCACTGAAGGACG
  CCCTGGGCAGACAGGTGAGCCTCCGGTCCTACGACAACATTCCCCCCACCAGCTCCTCCGACGAGGGCGA
  GGACGACGACGACGGCGAGGACGACGACAACGAGGAGCGGCAGCAGAAGCTCCGGCTGTGCGGATCCGGC
  TGCGGCGGCAACGACAGCAGCAGCGGGTCCCACCGGGAGGCCACTCACGACGGCCCCAAGAAAAACGCCG
  TGCGGAGCACCTTCCGGGAAGACAAGGCCCCCAAGCCCAGCAAGCAAAGCAAAAAGAAAAAGAAGCCCTC
  CAAACACCACCACCACCAGCAGAGCAGCATCATGCAGGAAACCGACGATCTGGACGAGGAGGACACAAGC
  ATCTACCTGAGCCCCCCCCCCGTGCCCCCCGTGCAGGTGGTGGCCAAGCGGCTGCCCCGGCCCGACACCC
  CACGGACCCCTCGGCAGAAGAAGATCAGCCAACGGCCACCCACCCCCGGAACCAAGAAACCTGCCGCCTC
  CCTGCCCTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101278 representing YP_081546
  Red=Cloning sites Green=Tags
  
  MGAELCKRICCEFGTTSGEPLKDALGRQVSLRSYDNIPPTSSSDEGEDDDDGEDDDNEERQQKLRLCGSG
  CGGNDSSSGSHREATHDGPKKNAVRSTFREDKAPKPSKQSKKKKKPSKHHHHQQSSIMQETDDLDEEDTS
  IYLSPPPVPPVQVVAKRLPRPDTPRTPRQKKISQRPPTPGTKKPAASLPF
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 570 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081546
• RefSeq ORF: 570
• Locus ID: 3077533
• MW: 20.9 kDa