US21 (NC_006273) Virus Tagged ORF Clone

CAT#: VC101338

Myc-DDK-tagged ORF clone of viral ORF for membrane protein US21 [Human herpesvirus 5], codon optimized for human cell expression, YP_081606

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: US21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101338 represents NCBI reference of YP_081606 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCCTGCGGGGGCAGGTGCAGATCGCACGGAGCGTGTTCCTCCTGCGGATCTACATTCTCATCTGGG
  TGCAGTGCCTGATTCTGATGAGCGTGTGCGCCTTTTGCTGGCTGGTGCTGCCCCACCGGCTGGAGCAGCT
  CTTCAGCAGCGTGCGGCTGACCCTGAGCTGTCTGATGATCAGCATCGTCTGCCTGGGCCTGCTGCGGTGG
  GCCGAGCCCAACTTCCCCAAGAACGTGTGGATCCTCCTGACCTACACCCTGCTGACCAGCGTGGCCGTGA
  CCGCCAGCGGCTTCCACTTCAGCCACCGGAGCGTGATCTACGCCATGGTGGCCACCGTCACTCTGTTCTG
  CTTCCTGACCCTGGCCACCTACCTGTTCGCCAGAGACGTGGAGCTCCAGCGGTCCCTGCTGACCGGCGCC
  AGCACTCTGATCCTCCTGCTGTTTGCCGTGTTCAGCCTCTTCCCCGAGGCCGTCAGCGAGATCCTGGTGA
  TGATCGCCGGCCTCGCTGTGATTGTGACAAGCGTCGTGTGCGACACTCAGGACATTCTGCACGACATCGA
  GTACGAGAGCTACATCCCCGGCGCACTGTGCCTGTACATGGACCTGATGTACCTGTTCGTGAGCGTGCTG
  TACTTCATGCCCTCCGAGCCTGGCAGCGCCCACACCGCACAGACAACCGTGGCTGCCACCGTCGCTGCCA
  CTGCCGCTGCAAGCCCCCAATTTGTGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101338 representing YP_081606
  Red=Cloning sites Green=Tags
  
  MSLRGQVQIARSVFLLRIYILIWVQCLILMSVCAFCWLVLPHRLEQLFSSVRLTLSCLMISIVCLGLLRW
  AEPNFPKNVWILLTYTLLTSVAVTASGFHFSHRSVIYAMVATVTLFCFLTLATYLFARDVELQRSLLTGA
  STLILLLFAVFSLFPEAVSEILVMIAGLAVIVTSVVCDTQDILHDIEYESYIPGALCLYMDLMYLFVSVL
  YFMPSEPGSAHTAQTTVAATVAATAAASPQFVS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_006273
• ORF Size: 729 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_006273.2, YP_081606
• RefSeq ORF: 729
• Locus ID: 3077437
• MW: 26.9 kDa